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p蛋白
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  p protein
    3.MC148P protein was obtained from the supernatant of 293 cells transfected with recombinant Ad-MC148 and served as antagonist against MCP-1 and RANTES.
    在第三部分中,Ad-MC 148腺病毒重组体转染293细胞大量扩增,上清中提纯MC 48P蛋白并测定其桔抗日趋化因子MCP-1和RANTES的生物学活性。
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  “p73蛋白”译为未确定词的双语例句
    At the same time, there was positive expression of S100 protein, phosphotyrosine (Ptye) and Src homolog region 2 protein(SH2P) in the heat injured fibroblasts. With the addition of FGF2, the expression of S100 protein and SH2P was weaker( P <0.05) , and expression of Ptyr was slightly weaker.
    同时,热损伤组成纤维细胞S100、 P-tyr蛋白、SH2-P蛋白表达阳性,FGF2组细胞S100蛋白和SH2-P的表达明显较弱(P<0.05)、P-tyr 的表达略有减弱。
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    Methods: S-P immunohistochemical method was used to detect expression of ki-67 protein in 30 cases of osteosarcoma and 10 cases of osteochondroma.
    方法:采用免疫组化S-P蛋白的表达情况。
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  p protein
The possible mechanisms of the formation of the modified ADH-P protein are discussed in connection with the differential activity of genes in the microgametophytes of angiosperms.
      
The immunoblot analysis with RSV-infected and non-infected HEp-2 cells as antigen revealed the expected age-dependent low prevalence of G protein antibodies and clear seroconversion of N and P protein antibodies.
      
Are anti-ribosomal P protein antibodies relevant in systemic lupus erythematosus
      
Cellular expression pattern of the glycine decarboxylase P protein in leaves of an intergeneric hybrid between the C3-C4 interme
      
The densities of NF150D and NF150P protein spots on the Coomassie blue-stained two-dimensional gels of the normal and injured cords also displayed alterations similar to the immunocytochemical data.
      
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objective: To evaluate the expression of ki-67 protein and its relation to carcinogenesis, development and pathological types of osteosarcoma . Methods: S-P immunohistochemical method was used to detect expression of ki-67 protein in 30 cases of osteosarcoma and 10 cases of osteochondroma. Results: The positive rate oj ki-67 protein was 66.67% in osteosarcoma and 10% in osteochondroma , comparing thf staining rate and intensity between them , respectively, there were all significant differences (P<0.05). The...

objective: To evaluate the expression of ki-67 protein and its relation to carcinogenesis, development and pathological types of osteosarcoma . Methods: S-P immunohistochemical method was used to detect expression of ki-67 protein in 30 cases of osteosarcoma and 10 cases of osteochondroma. Results: The positive rate oj ki-67 protein was 66.67% in osteosarcoma and 10% in osteochondroma , comparing thf staining rate and intensity between them , respectively, there were all significant differences (P<0.05). The positive immunostaining rate was 28.57% in higher differentiation group and 78.26% in lower differentiation group, comparing the staining rate and intensity oj higher and lower differentiation , respectively, there were all significant differences ( P<0.05) . Comparing the staining results of the pathological types, there all were not significant differences (P>0.05). Conclusion: The expression of ki-67 protein in deeply involved in the carcinogenesis and development of osteosarcoma . It can be a marker of malignant grade oj osteosarcoma .

目的:探讨ki-67蛋白在人骨肉瘤中的表达及其与骨肉瘤发生、发展和病理分型的关系。方法:采用免疫组化S-P蛋白的表达情况。结果:ki-67蛋白在骨肉瘤中阳性表达率为66.67%,在骨软骨瘤中阳性表达率为10%,比较骨肉瘤和骨软骨瘤染色阳性率和染色强度,差异均有显著性(P<0.05)。ki-67蛋白在骨肉瘤高分化组阳性表达率为28.57%,在骨肉瘤低分化组的阳性表达率为78.26%,两组染色阳性率及染色强度的差异均有显著性(P<0.05)。而ki-67蛋白在骨肉瘤各病理亚型的染色结果,差异无显著性(P>0.05)。结论:ki-67蛋白表达与骨肉瘤发生、发展密切相关,其阳性表达率和表达程度是判断骨肉瘤恶性程度的一个指标。

Objective To investigate the effects of fibroblast growth factor-2( FGF2) on S100 protein expression and the involvement of S100 protein in FGF-induced cell proliferation and anti-apoptosis effects in cultured fibroblasts after heat injury in vitro. Methods Human fibroblasts were cultured in Dulbecco's modified Eagles medium supplemented with 5% calf serum ( DMEM) at 37℃ in a humidified atmosphere of 5% CO2 and 95% air for 24 hours, then the cells were injured by heating to 45℃ for 10 min. They were divided...

Objective To investigate the effects of fibroblast growth factor-2( FGF2) on S100 protein expression and the involvement of S100 protein in FGF-induced cell proliferation and anti-apoptosis effects in cultured fibroblasts after heat injury in vitro. Methods Human fibroblasts were cultured in Dulbecco's modified Eagles medium supplemented with 5% calf serum ( DMEM) at 37℃ in a humidified atmosphere of 5% CO2 and 95% air for 24 hours, then the cells were injured by heating to 45℃ for 10 min. They were divided into two groups. In group one FGF2 (10 μg/L) was added, and group two served as negative control without the addition of FGF2. The cells were incubated at 37℃/5% CO2 for 30 min. Immunofluorescence double labeling of fibroblasts was carried out and the cells were observed under laser scanning confocal microscope. The intensity of fluorescence label in fibroblasts was measured using NIH Imagine software. Results Heat injured fibroblasts were found to be able to express both caspase-3 and proliferating cell nuclear protein( PCNA) , with respective gray scale of 140 ±31 and 52 ± 8. In group one, after the addition of FGF2, the heat injured fibroblasts were found to express these two proteins too, and with respective gray scale of 75±28 and 131±17. There was a statistically significant difference in values of PCNA between two groups( P < 0. 05). At the same time, there was positive expression of S100 protein, phosphotyrosine (Ptye) and Src homolog region 2 protein(SH2P) in the heat injured fibroblasts. With the addition of FGF2, the expression of S100 protein and SH2P was weaker( P <0.05) , and expression of Ptyr was slightly weaker. Conclusion Heat injury can induce apoptotic signals of fibroblasts in vitro. The role of FGF2 in the regulation of proliferation and anti-apoptosis in heat injured fibroblasts may be, at least in part, attributable to S100 proteins expression.

目的观察热损伤后成纤维细胞凋亡模型中,外用成纤维细胞生长因子2(FGF2)对凋亡细胞S100蛋白表达的影响。方法用含体积分数5%小牛血清的DMEM溶液培养已大致融合的成纤维细胞24 h,置入45℃恒温水浴锅中孵育10 min,建立热损伤细胞模型(热损伤组,3瓶),在上述基础上立即加入FGF2(FGF2组,10μg/L,3瓶)。两组细胞继续常规培养30 min后作免疫荧光双标记,利用激光扫描共聚焦显微镜观察细胞半胱氨酸天冬氨酸蛋白酶3(caspase-3)和增殖细胞核抗原(PCNA)以及S100蛋白、磷酸酪氨酸蛋白(P-tyr)、src基因产物蛋白同源的酪氨酸激酶催化功能区-2(SH2-P)表达的变化。结果热损伤组细胞可以同时表达caspase-3蛋白和PCNA两种抗体, 其灰度值分别为140±31、52±8;FGF2组细胞亦见上述两种抗体表达,灰度值分别为75±28、131± 17,PCNA表达量与热损伤组比较,差异有统计学意义(P<0.05)。同时,热损伤组成纤维细胞S100、 P-tyr蛋白、SH2-P蛋白表达阳性,FGF2组细胞S100蛋白和SH2-P的表达明显较弱(P<0.05)、P-ty...

目的观察热损伤后成纤维细胞凋亡模型中,外用成纤维细胞生长因子2(FGF2)对凋亡细胞S100蛋白表达的影响。方法用含体积分数5%小牛血清的DMEM溶液培养已大致融合的成纤维细胞24 h,置入45℃恒温水浴锅中孵育10 min,建立热损伤细胞模型(热损伤组,3瓶),在上述基础上立即加入FGF2(FGF2组,10μg/L,3瓶)。两组细胞继续常规培养30 min后作免疫荧光双标记,利用激光扫描共聚焦显微镜观察细胞半胱氨酸天冬氨酸蛋白酶3(caspase-3)和增殖细胞核抗原(PCNA)以及S100蛋白、磷酸酪氨酸蛋白(P-tyr)、src基因产物蛋白同源的酪氨酸激酶催化功能区-2(SH2-P)表达的变化。结果热损伤组细胞可以同时表达caspase-3蛋白和PCNA两种抗体, 其灰度值分别为140±31、52±8;FGF2组细胞亦见上述两种抗体表达,灰度值分别为75±28、131± 17,PCNA表达量与热损伤组比较,差异有统计学意义(P<0.05)。同时,热损伤组成纤维细胞S100、 P-tyr蛋白、SH2-P蛋白表达阳性,FGF2组细胞S100蛋白和SH2-P的表达明显较弱(P<0.05)、P-tyr 的表达略有减弱。结论离体情况下,热损伤可诱导成纤维细胞凋亡信号的表达;S100蛋白在 FGF2对热损伤导致的凋亡成纤维细胞的增殖和抗凋亡作用中扮演重要角色。

 
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