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yeast two-hybrid screen
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  “yeast two-hybrid screen”译为未确定词的双语例句
     CT120A could interact with SLC3A2 (member 2 of solute carrier family) and GGTL3B (isoform of y-glutamyltranspeptidase-like 3) in eukaryotic cells by yeast two-hybrid screen and co-immunoprecipitation assay,which suggested that CT120 might assume very essential physiological functions involved in amino acid transport and glutathione metabolism.
     CT120A在细胞内可与SLC3A2和GGTL3B相互作用,可能拥有与氨基酸转运及谷胱苷肽代谢有关的重要的生理功能。
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     Yeast two-hybrid screen showed LMW-DSP2 interacted with RAB11A.
     通过酵母双杂交筛选人类胎脑文库,发现LMW-DSP2蛋白与参加囊泡运输的RAB11A具有相互作用。
短句来源
     The sequence of a cDNA clone, identified positively in a yeast two-hybrid Screen using human ubiquitin conjugating enzyme CDC34 as a bait, was found to have the ability of co-immuno-precipitation with protein kinase C related kinase 1(PRK1 or PKN) in mammalian cells.
     用人Ubiquitin结合酶CDC34作诱饵,从一酵母双杂交系统中分离出来的阳性克隆之一,能够与哺乳动物细胞的PRK1(protein kinase C related kinase 1,PRK1,又称PKN)共免疫沉淀。
短句来源
     Methods ERβ was cloned into the yeast expression vector pGBKT7. The standard yeast two-hybrid screen was performed to isolate the protein interacting with ERβ.
     方法:将ERβ基因克隆入酵母表达载体pGBKT7中,利用酵母双杂交筛选技术,从乳腺文库中分离与ERβ相互作用的蛋白质。
短句来源
     CT120 can interact with SLC3A2 (member 2 of solute carrier family 3) and GGTL3B (isoform of γ-glutamyltranspeptidase-like 3) in eukaryotic cells by yeast two-hybrid screen and co-immunoprecipitation assay, which suggested that CT120 may assume very essential physiological functions involved in amino acid transport and glutathione metabolism.
     CT120在细胞内可与SLC3A2和GGTL3B相互作用,可能拥有与氨基酸转运及谷胱苷肽代谢有关的重要的生理功能。 为了进一步研究CT120与肺癌发生发展的关系,我们从蛋白质水平研究CT120在肺癌及癌旁组织中的差异表达及CT120异位表达与过表达对细胞生长的影响。
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  相似匹配句对
     Two.
     二、末日意识
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     Two.
     二.
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     The yeast two-hybrid system and its development
     酵母双杂交系统及发展
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     Yeast Two-hybrid Derived System
     酵母双杂交衍生系统
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     Yeast two-hybrid system and its applications
     酵母双杂交系统及其应用
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  yeast two-hybrid screen
To gain more insight in its function, we initiated a yeast two-hybrid screen.
      
In the present study, we identified Nmi (N-Myc and STATs Interactor) as a novel binding partner for Tip60 by a yeast two-hybrid screen.
      
To identify MEOX2 binding proteins, a yeast two-hybrid screen of a human heart cDNA library was performed using a deleted MEOX2 bait protein that does not contain the histidine/glutamine rich region (MEOX2ΔHQ).
      
We have identified OZF interacting factors with a yeast two-hybrid screen.
      
A yeast two-hybrid screen using H11 kinase as a bait in a human heart library revealed a potential interaction with phosphoglucomutase (PGM), the enzyme converting glucose 6-phosphate into glucose 1-phosphate.
      
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Using hepatopioetin (HPO) as "bait" in an yeast two hybrid screen resulted in the identification of JAB1(a protein initially as a co activator of c Jun) as a putative HPO binding partner.Then the full length cDNA of JAB1 was obtained from human fetal liver cDNA library by PCR for GST JAB1 expression.The binding assay showed that JAB1 binded to rHPO and HPO expressed in COS7 cells in vitro .

利用酵母双杂交方法 ,用肝细胞生成素 (HPO)作为诱饵蛋白在人胎肝cDNA文库中筛选到能与HPO相互作用的蛋白 :AP 1辅助激活因子JAB1.并用PCR方法从人胎肝cDNA文库中扩出JAB1全长cDNA ,进行GST JAB1原核融合蛋白表达与纯化 .蛋白质结合实验表明 ,JAB1与人重组HPO以及COS7真核表达的HPO在体外有结合作用

The authors used COI 1 as bait to screen Arabidopsis cDNA library in yeast two-hybrid system. More than 300 putative positive colonies have been isolated,which belonged to 12 distinct groups. Representative cDNAs from each group were sequenced. DNA database searching showed that one of these genes, represented by 59 positive colony, was identical to the previously identified Arabidopsis Skp1 like protein ASK1 (formerly named as AtSkp 1).Other putative COI 1 associated proteins were designated as COAP2, 3 to...

The authors used COI 1 as bait to screen Arabidopsis cDNA library in yeast two-hybrid system. More than 300 putative positive colonies have been isolated,which belonged to 12 distinct groups. Representative cDNAs from each group were sequenced. DNA database searching showed that one of these genes, represented by 59 positive colony, was identical to the previously identified Arabidopsis Skp1 like protein ASK1 (formerly named as AtSkp 1).Other putative COI 1 associated proteins were designated as COAP2, 3 to 12, respectively. COI 1 interacted strongly with ASK1, COAP2 and COAP3.Presence of the F-box motif in COI 1 led us to the prediction that COI 1 might interact with Skp 1-like protein to form SCF ubiquitin complex.Indeed,ASK1,COAP2 and COAP3 identified in the yeast two-hybrid screen are similar to the Skp 1 protein from yeast and human.The authors therefore focused their analysis on ASK1,COAP2, COAP3 and COAP4 with the complementary biochemical and molecular approaches.

为进一步阐明茉莉素调控植物生长发育的分子机理 ,为作物抗性和雄性不育基因工程提供新思路 ,以COI 1为诱饵 ,利用酵母双杂交分析体系筛选拟南芥 c DNA文库 ,得到 30 0多个阳性克隆 ,经 PCR,Rsa 酶切 PCR产物以后归为 12群 ,从代表菌落提取 p B42 AD c DNA重组质粒进行了测序 ,完成了序列分析 ,得到 ASK1,COAP2 ,COAP3,COAP4等 12个基因 ,其中 ASK1,COAP2 ,COAP3等基因与 Skp1等重要基因有较高的同源性 .

Objective To construct and transform yeast bait plasmids carrying the androgen receptor-associated protein 267-α (ARA267-α) cDNA fragments.Methods Fragments of ARA267-αcDNA that respectively code the PWWP and PHD-SET conserved domains were amplified using PCR and directionally ligated to the pGBKT7 vector.Insert-contained plasmids were confirmed by restriction analysis and DNA sequencing and then transformed into AH109 yeast strain.Results pGBKT7-PWWP and pGBKT7-PHDSET recombinant plasmids were...

Objective To construct and transform yeast bait plasmids carrying the androgen receptor-associated protein 267-α (ARA267-α) cDNA fragments.Methods Fragments of ARA267-αcDNA that respectively code the PWWP and PHD-SET conserved domains were amplified using PCR and directionally ligated to the pGBKT7 vector.Insert-contained plasmids were confirmed by restriction analysis and DNA sequencing and then transformed into AH109 yeast strain.Results pGBKT7-PWWP and pGBKT7-PHDSET recombinant plasmids were successfully constrcted.All three sorts of yeasts respectively transformed recombinant plasmids and empty pGBKT7 vector could grow white colonies on SD/-Trp/ X-α-gal plates and none could survive on SD/-His/-Trp/2.5 mmol/L 3-AT/X-α-gal and SD/-Ade/-Trp/X-α-gal plates.After being cultured in SD/-Trp liquid medium for 16 h the A 600 of them was 0.8,0.9,0.9 respectively.This indicated the fusion proteins expressed by recombinant plasmids did not activate the expression of yeast reporter genes HIS3,ADE2 and MEL1 and had no toxicity to yeast strain.Conclusion The bait plasmids constructed can be used to study the function of ARA267-αin Yeast Two-Hybrid Screen.

目的 构建和转化雄激素受体相关蛋白 2 67 α(ARA2 67 α)的酵母诱饵重组质粒 ,为通过酵母双杂交研究ARA2 67 α的功能奠定基础。方法 用PCR扩增ARA2 67 α全长cDNA中编码PWWP和PHD SET蛋白结构域的两个基因片段 ;将基因片段与 pGBKT7载体定向重组 ;用酶切和测序鉴定重组质粒 ;将核苷酸序列正确的重组质粒转化入AH 10 9酵母菌株。结果 成功构建pGBKT7 PWWP和 pGBKT7 PHDSET重组质粒。分别转化有 2种重组质粒和pGBKT7空载体的3种AH10 9酵母都能在SD/ Trp/X α gal培养基中长成白色菌落 ,但都不能在SD/ His/ Trp/2 .5mmol/L 3 AT/X α gal和SD/ Ade/ Trp/X α gal培养基中生长 ,在SD/ Trp液中培养 16h后 ,A60 0均值分别为 0 .8、0 .9、0 .9。这表明重组质粒表达的融合蛋白没有激活HIS3、ADE2和MEL1酵母报告基因表达的活性 ,也没有酵母毒性作用。结论 构建的诱饵重组质粒可以用于下一阶段的cDNA文库筛选

 
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