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prolong expression
相关语句
  持续表达
     The strategies of gene prolong expression in mammals
     哺乳动物中基因持续表达的策略
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  相似匹配句对
     Expression of E.
     将阳性重组质粒转化表达受体菌E.
短句来源
     The expression of E.
     研究了E.
短句来源
     The strategies of gene prolong expression in mammals
     哺乳动物中基因持续表达的策略
短句来源
     A transposon system was also used to prolong the insulin expression in the liver.
     实验还采用了转座元系统来延长肝脏中胰岛素的表达。
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Objective To investigate the potential of exogenously expressed CTLA4-FasL in inducing transplantation tolerance using rat cardiac graft model and its related mechanisms. Methods The heart allograft of DA rat was placed in the abdomen of LEW rat, and adenoviruses containing CTLA4-FasL gene (AdCTLA4-FasL) adenovirus containing CTLA4Ig, and AdEGPP were infused at a dose of 5×109 pfu/ml via portal vein in different recipients respectively immediately after the operation. DA→LEW cardiac graft controls and syngeneic...

Objective To investigate the potential of exogenously expressed CTLA4-FasL in inducing transplantation tolerance using rat cardiac graft model and its related mechanisms. Methods The heart allograft of DA rat was placed in the abdomen of LEW rat, and adenoviruses containing CTLA4-FasL gene (AdCTLA4-FasL) adenovirus containing CTLA4Ig, and AdEGPP were infused at a dose of 5×109 pfu/ml via portal vein in different recipients respectively immediately after the operation. DA→LEW cardiac graft controls and syngeneic LEW-LEW cardiac graft controls were used. The survival of cardiac allografts was monitored by daily palpation. The total cessation of beating was defined as rejection and was confirmed by histology. The serum level of CTLA4-FasL was measured via ELISA. The tolerance mechanisms were investigated with adoptive transfer, mixed lymphocyte reaction(MLR), IL-2 reverse experiment, determinations of frequencies of HTLp and CTLp, and analysis of the polarization of TH1/TH2 type cytokines using RT-PCR. Results The survival of DA allografts were prolonged significantly in LEW recipients receiving AdCTLA4-FasL with a mean survival time of 71.0±23.7d (n=6), significantly longer than those of the untreated recipients (5.7±0.5d, n=6), AdEGFP-treated recipients (5.2±0.4d, n=6) and AdCTLA4Ig-treated recipients (45.7±12.4d, n=6) (call P<0.05). Prolonged expression of serum CTLA4-FasL was shown in AdCTLA4-FasL-treated rats. Splenocytes of LEW recipients with long-term surviving cardiac allograft displayed donor-specific hyporesponsiveness, which could not be reversed in the presence of exogenous added IL-2 in MLR. Frequencies of HTLp and CTLp were significantly reduced. The polarization of TH1/TH2 type cytokines was not shown. Conclusion Adenovirus-mediated CTLA4-FasL gene transfer renders prolonged therapeutic expression of CTLA4-FasL in LEW recipient rats, leading to long-term survival of cardiac allografts. The induced tolerance is donor-specific, and may result from regulatory T cells and the deletion of alloreactive T cells. However, T cell anergy and the deviation of TH1/TH2 type cytokines may not be the involved mechanism, at least when tested.

目的 探讨腺病毒介导细胞毒T淋巴细胞相关抗原 (CTLA4 ) FasL基因转移延长异基因大鼠心脏移植物存活的作用及其相关机制。方法 供体DA大鼠心脏移植给受体LEW大鼠后 ,经门静脉注入 5× 10 9空斑形成单位 /毫升 (pfu/ml)CTLA4 FasL腺病毒 (AdCTLA4 FasL) ;随后 ,通过每天触摸受体大鼠腹壁 ,记录心脏移植物存活时间。受体大鼠尾静脉取血 ,用酶联免疫吸附法(ELISA)测定CTLA4 FasL融合蛋白的体内表达 ;通过过继性转移试验、混合淋巴细胞反应 (MLR)及白细胞介素 (IL) 2逆转实验、细胞毒T细胞 (CTL)活性测定、辅助性T淋巴细胞前体 (HTLp)和细胞毒T淋巴细胞前体 (CTLp)频数测定及辅助性T细胞 (TH) 1/ 2型细胞因子检测 ,探讨耐受机制。结果 经AdCTLA4 FasL处理的受体大鼠 ,异基因心脏移植物平均存活时间达 (71 0± 2 3 7)d(n =6 ) ,而对照的未处理组、绿色荧光蛋白 (EGFP)腺病毒处理组和细胞毒T淋巴细胞相关抗原 4免疫球蛋白 (CTLA4Ig)腺病毒处理组平均存活时间分别为 (5 7± ...

目的 探讨腺病毒介导细胞毒T淋巴细胞相关抗原 (CTLA4 ) FasL基因转移延长异基因大鼠心脏移植物存活的作用及其相关机制。方法 供体DA大鼠心脏移植给受体LEW大鼠后 ,经门静脉注入 5× 10 9空斑形成单位 /毫升 (pfu/ml)CTLA4 FasL腺病毒 (AdCTLA4 FasL) ;随后 ,通过每天触摸受体大鼠腹壁 ,记录心脏移植物存活时间。受体大鼠尾静脉取血 ,用酶联免疫吸附法(ELISA)测定CTLA4 FasL融合蛋白的体内表达 ;通过过继性转移试验、混合淋巴细胞反应 (MLR)及白细胞介素 (IL) 2逆转实验、细胞毒T细胞 (CTL)活性测定、辅助性T淋巴细胞前体 (HTLp)和细胞毒T淋巴细胞前体 (CTLp)频数测定及辅助性T细胞 (TH) 1/ 2型细胞因子检测 ,探讨耐受机制。结果 经AdCTLA4 FasL处理的受体大鼠 ,异基因心脏移植物平均存活时间达 (71 0± 2 3 7)d(n =6 ) ,而对照的未处理组、绿色荧光蛋白 (EGFP)腺病毒处理组和细胞毒T淋巴细胞相关抗原 4免疫球蛋白 (CTLA4Ig)腺病毒处理组平均存活时间分别为 (5 7± 0 5 )d(n =6 )、(5 2± 0 4 )d(n =6 )和 (4 5 7± 12 4 )d(n =6 ) ,差异具有显著意义 (均P <0 0 5 )。心脏移植物长期存活的受体大鼠对供体抗原的低应答可以被过继转移 ;MLR活性特异性降低 ,且不被外源性IL 2逆转 ;CTL活?

To counter the non standard or wrong titles in official document drafting, this article analyses the eight aspects of abuse of omission, misusage of writing style, abnormal grammar rule, vague statement, prolonged expression, improper application, misusage of punctuation marks and confusion of typefaces according to the regulations in Official Document Handling Ways of National Administrative Institution and Official Document Forms for National Administrative Institution. It puts forward basic elements...

To counter the non standard or wrong titles in official document drafting, this article analyses the eight aspects of abuse of omission, misusage of writing style, abnormal grammar rule, vague statement, prolonged expression, improper application, misusage of punctuation marks and confusion of typefaces according to the regulations in Official Document Handling Ways of National Administrative Institution and Official Document Forms for National Administrative Institution. It puts forward basic elements and standardized requirements in the titles of official document drafting from different angles.

针对公文拟制过程中出现的不规范或错误的标题 ,从滥行省略、文种错用、语法不规范、表述不清、表达不简洁、适用不当、标点符号乱用、体号混乱八个方面 ,按《国家行政机关公处理办法》《国家行政机关公文格式》的规定加以分析 ,从多个角度提出了公文标题拟制的基本要素和规范要求

Objective To investigate the expressions of heat shock protein 70 (HSP70) in neurons and oligodendrocytes after the stretch injury and the effects of heat stress on HSP70 expression and explore neuroprotective effect of HSP70 on axonal injury. Methods Fifty-seven male Wistar rats were randomly divided into four groups: control group (Group A with three rats), stretch-only group (Group B with 18 rats), heat stress-only group (Group C with 18 rats) and heat stress pretreatment plus stretch...

Objective To investigate the expressions of heat shock protein 70 (HSP70) in neurons and oligodendrocytes after the stretch injury and the effects of heat stress on HSP70 expression and explore neuroprotective effect of HSP70 on axonal injury. Methods Fifty-seven male Wistar rats were randomly divided into four groups: control group (Group A with three rats), stretch-only group (Group B with 18 rats), heat stress-only group (Group C with 18 rats) and heat stress pretreatment plus stretch group (Group D with 18 animals). Stretch injury was induced in the right optic nerves of rats in Group B. Heat stress was applied to animals of Group C. Animals in Group D were subjected to the same stretch injury as Group B posterior to the heat stress similar to Group C. Three animals respectively from each group of Group B, Group C and Group D were killed at 4, 8 and 16 hours, 1, 3 and 5 days, respectively. Morphological changes of optic nerves, retinal ganglion cells (RGCs) and oligodendrocytes (OLs) in optic nerves after stretch injury were examined under light microscope; and expressions of OLs HSP70 in RGCs and OLs after heat stress and/orstretch injury were observed using immunohistochemistry. Results Pathological changes of axons, RGCs and OLs after stretch injury to the optic nerves were identified morphologically or quantitatively, and significantly ameliorated by pre-treatment with heat stress before stretch injury. An increased expression of OLs HSP70 and RGCs occurred in Groups B and C. A much more enhanced expression of HSP70 was found in Group D with an earlier peak and longer maitenance period. Conclusions Both neurons and glial cells are involved in pathological process after axonal injury. Heat stress can more effectively exert the neuroprotective role through enhancing, bringing forward and prolonging expression of HSP70. A new approach to management of DAI may be developed through the enhancement of intrinsic neuroprotective functions.

目的 观察轴索损伤后相应的神经元及少突胶质细胞热休克蛋白70 (HSP70)表达的变化及热应激对其表达的影响,探讨HSP70的神经保护作用。 方法 57只雄性Wistar大鼠随机分为正常对照组(A组, 3只)、单纯视神经牵拉伤组(B组, 18只)、单纯热应激处理组(C组,18只)和热应激预处理牵拉伤组(D组, 18只)。对B组大鼠右侧视神经施予牵拉,对C组大鼠施予热应激处理,D组大鼠热应激预处理24h后再对右侧视神经施予牵拉,B、C、D组分别在4, 8,16h、1, 3, 5d各处死3只大鼠。光镜下观察视神经、视网膜神经节细胞(RGCs)、视神经少突胶质细胞(OLs)的形态学变化,免疫组化染色检测RGCs及OLsHSP70表达情况。 结果 牵拉伤后视神经轴索、RGCs及OLs的形态发生明显的病理变化,热应激预处理再致伤后上述病理改变有显著改善。单纯牵拉伤和单纯热应激处理均可使RGCs及OLsHSP70的表达增加,而热应激预处理再致伤使HSP70的表达明显增强,高峰表达时间提前,维持时间延长。 结论 神经元及胶质细胞共同参与了轴索损伤后的病理过程。热应激能使HSP70表达增强、提前和延长,提高其神经保护作用。增...

目的 观察轴索损伤后相应的神经元及少突胶质细胞热休克蛋白70 (HSP70)表达的变化及热应激对其表达的影响,探讨HSP70的神经保护作用。 方法 57只雄性Wistar大鼠随机分为正常对照组(A组, 3只)、单纯视神经牵拉伤组(B组, 18只)、单纯热应激处理组(C组,18只)和热应激预处理牵拉伤组(D组, 18只)。对B组大鼠右侧视神经施予牵拉,对C组大鼠施予热应激处理,D组大鼠热应激预处理24h后再对右侧视神经施予牵拉,B、C、D组分别在4, 8,16h、1, 3, 5d各处死3只大鼠。光镜下观察视神经、视网膜神经节细胞(RGCs)、视神经少突胶质细胞(OLs)的形态学变化,免疫组化染色检测RGCs及OLsHSP70表达情况。 结果 牵拉伤后视神经轴索、RGCs及OLs的形态发生明显的病理变化,热应激预处理再致伤后上述病理改变有显著改善。单纯牵拉伤和单纯热应激处理均可使RGCs及OLsHSP70的表达增加,而热应激预处理再致伤使HSP70的表达明显增强,高峰表达时间提前,维持时间延长。 结论 神经元及胶质细胞共同参与了轴索损伤后的病理过程。热应激能使HSP70表达增强、提前和延长,提高其神经保护作用。增强内源性神经保护作用是弥漫性轴索损伤(DAI)治疗的新途径。

 
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