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bone matrix material
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  骨基质材料
     To design and manufacture a new bone matrix material (NBM) composed of both organic and inorganic materials for bone tissue engineering, the osteogenic potential of NBM combined with osteoblasts was studied in vitro and in vivo. Osteoblasts from bone marrow stromal cells of New Zealand rabbit were cultured with NBM in vitro , then the materials combined with osteoblasts were implanted into the skeletal muscles of rabbits. The osteogenic potential of NBM was evaluated using contrast microscope, scan electromicroscope and histological examination.
     为评价一种新型无机 有机复合骨基质材料 (newbone matrixmaterial,NBM)的体内外成骨能力 ,取骨髓基质干细胞来源的成骨细胞与材料进行体外复合培养 ,复合体再行肌内植入 ,通过相差显微镜、扫描电镜以及组织学观察体内外成骨情况。
短句来源
     Objective: A compound new bone matrix material (NBM) composed of both organic and inorganic materials was designed for bone tissue engineering, and the biocompatibility of NBM with bone marrow stromal cells (BMSC) was investigated.
     目的 :新型有机 无机复合类骨基质材料 (NewBone matrixMaterial,NBM)的研制及其细胞相容性的初步检测 ,为骨组织工程探寻一种新型复合类细胞外基质材料提供实验依据。
短句来源
     Conclusion: NBM is a new compound bone matrix material constituted of organic and inorganic materials, which pocesses a morphological structure of three dimensional foam.
     结论 :NBM是一种新研制的有机 无机复合类骨基质材料 ,具有网状孔隙结构 ;
短句来源
     Objective To evaluate the biocompatibility of a new bone matrix material (NBM) composed of both organic and inorganic materials for bone tissue engineering.
     目的 评价一种新型无机 -有机复合类骨基质材料 (NBM)的生物相容性。
短句来源
  相似匹配句对
     Biocompatibility of Tri-block Bone-matrix Material in vitro
     三嵌段骨基质材料的体外生物学性能评价
短句来源
     The development of cell matrix material used in bone tissue engineering
     骨组织工程中细胞种植基质材料的发展
短句来源
     Material
     实验材料与方法
短句来源
     Material
     实验材料
短句来源
     ②VECs group, which compounded VECs with acellular matrix bone material.
     ②血管内皮细胞组:血管内皮细胞与脱细胞基质骨材料复合。
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  bone matrix material
Cell deformations are predicted from detailed linear FE analysis of the microstructure, consisting of an arrangement of cells embedded in bone matrix material.
      


To design and manufacture a new bone matrix material (NBM) composed of both organic and inorganic materials for bone tissue engineering, the osteogenic potential of NBM combined with osteoblasts was studied in vitro and in vivo. Osteoblasts from bone marrow stromal cells of New Zealand rabbit were cultured with NBM in vitro , then the materials combined with osteoblasts were implanted into the skeletal muscles of rabbits. The osteogenic potential of NBM was evaluated...

To design and manufacture a new bone matrix material (NBM) composed of both organic and inorganic materials for bone tissue engineering, the osteogenic potential of NBM combined with osteoblasts was studied in vitro and in vivo. Osteoblasts from bone marrow stromal cells of New Zealand rabbit were cultured with NBM in vitro , then the materials combined with osteoblasts were implanted into the skeletal muscles of rabbits. The osteogenic potential of NBM was evaluated using contrast microscope, scan electromicroscope and histological examination. Osteoblasts could attach and proliferate well in the NBM, secrecting lots of extracellular matrix, while in vivo experiment of the NBM in osteoblasts group showed that a large number of lymphacytes and phagocytes invaded into the inner of the material in the rabbit skeletal muscle beds after 4 weeks of implantation, no new bone formation was observed after 8 weeks. The different osteogenic potential expressed by NBM between in vitro and in vivo may be due to the immunogenity of NBM which causes cellular immunoreaction to destroy the osteogenic environment. More attention should be paid to the immunoreaction problem in tissue engineering between the host and organic inorganic composite materials.

为评价一种新型无机 有机复合骨基质材料 (newbone matrixmaterial,NBM)的体内外成骨能力 ,取骨髓基质干细胞来源的成骨细胞与材料进行体外复合培养 ,复合体再行肌内植入 ,通过相差显微镜、扫描电镜以及组织学观察体内外成骨情况。结果发现 ,体外培养时成骨细胞与材料可发生良好的粘附、增殖 ,并分泌大量细胞外基质成分 ,而细胞 材料复合体肌内植入后 4周可见大量淋巴细胞和巨噬细胞浸润 ,8周仍未见新生骨组织生成。作者认为NBM的这种体内外成骨能力的差异可能与NBM具有免疫原性 ,引起宿主免疫反应 ,从而丧失体内成骨环境有关

Objective: A compound new bone matrix material (NBM) composed of both organic and inorganic materials was designed for bone tissue engineering, and the biocompatibility of NBM with bone marrow stromal cells (BMSC) was investigated. Methods: The morphological characteristics of BMSC cultured with NBM in vitro was observed using phase contrast microscope and scanning electron microscope. Results: BMSC could attach and proliferate well on the NBM, secrecting lots of extracellular...

Objective: A compound new bone matrix material (NBM) composed of both organic and inorganic materials was designed for bone tissue engineering, and the biocompatibility of NBM with bone marrow stromal cells (BMSC) was investigated. Methods: The morphological characteristics of BMSC cultured with NBM in vitro was observed using phase contrast microscope and scanning electron microscope. Results: BMSC could attach and proliferate well on the NBM, secrecting lots of extracellular matrix. Conclusion: NBM is a new compound bone matrix material constituted of organic and inorganic materials, which pocesses a morphological structure of three dimensional foam. NBM showed a good biocompatibility with BMSC in vitro. There was no signs of inhibition of NBM material to the attachment, proliferation and functional expression of BMSC.

目的 :新型有机 无机复合类骨基质材料 (NewBone matrixMaterial,NBM)的研制及其细胞相容性的初步检测 ,为骨组织工程探寻一种新型复合类细胞外基质材料提供实验依据。方法 :应用组织培养技术 ,对兔骨髓基质干细胞 (BoneMarrowStromalCell,BMSC)和NBM体外联合培养进行相差显微镜观察和扫描电镜观察。结果 :骨髓基质干细胞可以在NBM材料上发生良好的粘附、增殖 ,并能长入材料的孔隙内 ,分泌大量的细胞外基质成份。结论 :NBM是一种新研制的有机 无机复合类骨基质材料 ,具有网状孔隙结构 ;在体外与BMSC复合培养时表现出良好的细胞相容性 ,对细胞的粘附、增殖和功能表达未见明显影响。

Objective To evaluate the biocompatibility of a new bone matrix material (NBM) composed of both organic and inorganic materials for bone tissue engineering. Methods Osteoblasts combined with NBM in vitro were cultured. The morphological characteristics was observed; cell proliferation, protein content and basic alkaline phosphatase(ALP) activity were measured. NBM combined with osteoblasts were implanted into the skeletal muscles of rabbits and the osteogenic potential of NBM...

Objective To evaluate the biocompatibility of a new bone matrix material (NBM) composed of both organic and inorganic materials for bone tissue engineering. Methods Osteoblasts combined with NBM in vitro were cultured. The morphological characteristics was observed; cell proliferation, protein content and basic alkaline phosphatase(ALP) activity were measured. NBM combined with osteoblasts were implanted into the skeletal muscles of rabbits and the osteogenic potential of NBM was evaluated through contrast microscope, scanning electromicroscope and histological examination. Results In vitro osteoblasts could attach and proliferate well in the NBM, secreting lots of extracellular matrix; NBM did not cause the inhibition of proliferation and ALP activity of osteoblasts. While in vivo experiment of the NBM with osteoblasts showed that a large number of lymphacytes and phagocytes invading into the inner of the material in the rabbit skeletal muscle were seen after 4 weeks of implantation and that no new bone formation was observed after 8 weeks. Conclusion This biocompatibility difference between in vitro and in vivo may be due to the immunogenity of NBM which causes cellular immunoreaction so as to destroy the osteogenic environment. The immunoreaction between the host and the organic inorganic composite materials in tissue engineering should be paid more attention to.

目的 评价一种新型无机 -有机复合类骨基质材料 (NBM)的生物相容性。 方法 应用组织培养技术对 NBM体外复合兔成骨细胞培养 1~ 14天 ,然后进行形态学观察、细胞增殖、细胞蛋白含量与碱性磷酸酶 (AL P)活性测定 ;对 NBM采用同种兔体内植入观察 2、4和 8周 ,通过倒置显微镜、扫描电镜及组织学观察其体内成骨情况。 结果 体外培养时 NBM对成骨细胞的体外增殖和 AL P的表达无明显抑制作用 ,成骨细胞与材料可良好黏附、增殖 ,并分泌大量细胞外基质成分 ;而细胞 -材料复合体肌内植入后 4周可见大量淋巴细胞和巨噬细胞浸润 ,8周仍未见新生骨组织生成。 结论  NBM材料的体内外生物相容性差异可能与 NBM免疫原性有关 ,体内植入后引起宿主免疫反应 ,影响体内成骨。无机 -有机复合材料引起的移植免疫排斥反应应引起组织工程研究的注意。

 
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