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microbial total
相关语句
  微生物总
     Extraction of Rabbit Cecum Microbial Total DNA
     家兔盲肠微生物总DNA的提取
短句来源
     Four methods to purify soil microbial total DNA extracted directly from soil were compared. The results showed that purification method with 1% Sepharose electrophoresis and with Sephadex G-200 centrifuge filtration could not completely purify total DNA directly extracted from soil microbes.
     比较了4种从土壤中直接抽提的微生物总DNA的纯化方法,实验结果表明1 %的琼脂糖凝胶电泳纯化方法及葡聚糖凝胶G 2 0 0离心层析纯化方法均不能完全纯化从土壤中抽提的微生物总DNA。
短句来源
  “microbial total”译为未确定词的双语例句
     Extraction and Purification of Soil Microbial Total DNA
     土壤微生物总DNA的提取和纯化
短句来源
     Comparison of Four Purification Methods for Soil Microbial Total DNA
     四种土壤微生物总DNA的纯化方法的比较
短句来源
     Soil microbial total DNA could not be obtained through DNA gel extraction kit after purification with 1% Sepharose containing 2% of PVP (polyvinylpyrrolidone) agarose gel electrophoresis.
     含2 %PVP的1 %琼脂糖凝胶电泳纯化,用DNA凝胶回收试剂盒回收后没有得到纯化后的土壤微生物总DNA。
短句来源
     Results showed that there were large differences of marine microbial total numbers between different sampling sites, and the melt sea ice and salinity in sea water were probably two important factors affecting the marine microbial amounts in Chukchi Sea.
     调查结果还显示 ,不同站位间的海洋微生物总量存在明显差异 ,海冰的融化和海水中的盐度可能是影响这一海区海洋微生物总量的重要因素。
短句来源
     As to marine bacteria in Bering sea, their detection rates were 100% and their amounts were generally 10 2-10 3 cells/cm 3. For marine fungi, their detection rates were more than 84% and their amounts generally 10 2-10 3 cells/cm 3. Similar to the situation in Chukchi Sea, there were fairly large differences of marine microbial total numbers between different sampling sites in Bering Sea, too.
     在白令海 ,海洋细菌的检出率为 1 0 0 % ,其总量一般在 1 0 2 — 1 0 3 cells/cm3 ; 海洋真菌的检出率 >84% ,其总量一般也在 1 0 2 — 1 0 3 cells/cm3 。
短句来源
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  相似匹配句对
     Extraction of Rabbit Cecum Microbial Total DNA
     家兔盲肠微生物总DNA的提取
短句来源
     Extraction and Purification of Soil Microbial Total DNA
     土壤微生物总DNA的提取和纯化
短句来源
     total ISp].
     total 15μl。
短句来源
     (E)total occlusion.
     E组:完全闭塞。
短句来源
     Microbial Flocculant
     微生物絮凝剂
短句来源
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Investigations of total number of marine microbes in low temperatures of Chukchi Sea and Bering sea were conducted during the summer time in north hemisphere. In Chukchi Sea the detection rates of marine bacteria and fungi were 100% and more than 94%, respectively. And the quantity of marine bacteria generally was more than 10 3 cells/cm 3, and that of marine fungi usually 10-10 3 cells/cm 3. In most investigation sites marine bacterial total numbers were higher than marine fungal total numbers. There are...

Investigations of total number of marine microbes in low temperatures of Chukchi Sea and Bering sea were conducted during the summer time in north hemisphere. In Chukchi Sea the detection rates of marine bacteria and fungi were 100% and more than 94%, respectively. And the quantity of marine bacteria generally was more than 10 3 cells/cm 3, and that of marine fungi usually 10-10 3 cells/cm 3. In most investigation sites marine bacterial total numbers were higher than marine fungal total numbers. There are abundant marine microorganisms distributing in the areas from surface water to deeper layers of 10m or 30m depth in Chukchi Sea. Results showed that there were large differences of marine microbial total numbers between different sampling sites, and the melt sea ice and salinity in sea water were probably two important factors affecting the marine microbial amounts in Chukchi Sea. As to marine bacteria in Bering sea, their detection rates were 100% and their amounts were generally 10 2-10 3 cells/cm 3. For marine fungi, their detection rates were more than 84% and their amounts generally 10 2-10 3 cells/cm 3. Similar to the situation in Chukchi Sea, there were fairly large differences of marine microbial total numbers between different sampling sites in Bering Sea, too.The investigation results proved that a large number of marine microbes in low temperatures lived in Chukchi and Bering Sea. Among the marine bacteria studied, 81% of bacteria from Chukchi Sea and 88.9% of bacteria from Bering sea can grow well in low temperatures no higher than 10℃. And some of these marine bacteria can utilize starch or cellulose as carbon source for their growth. These microbes can provide abundant materials for further exploiting and developing the marine microbe resources in low temperature.

在 1 999年北极夏季期间对楚科奇海和白令海的海洋低温微生物进行了调查。在楚科奇海 ,海洋细菌和海洋真菌的检出率分别为 1 0 0 %和 >94% ,其相应的总量一般分别为 >1 0 3 cells/cm3 和 1 0 1— 1 0 3 cells/cm3 ;在大多数站位 ,海洋细菌的总量通常都高于海洋真菌的总量 ;从表层至 1 0 m或 3 0 m深层的海水区域 ,分布有丰富的海洋微生物。调查结果还显示 ,不同站位间的海洋微生物总量存在明显差异 ,海冰的融化和海水中的盐度可能是影响这一海区海洋微生物总量的重要因素。在白令海 ,海洋细菌的检出率为 1 0 0 % ,其总量一般在 1 0 2 — 1 0 3 cells/cm3 ;海洋真菌的检出率 >84% ,其总量一般也在 1 0 2 — 1 0 3 cells/cm3 。白令海不同站位间的海洋微生物总量也存在较大的差异。调查结果证实在楚科奇海和白令海的确存在大量的低温海洋微生物 ,在所研究的海洋细菌中 ,分别有 81 %的楚科奇海细菌和 88.9%的白令海细菌能够在低温条件下 (<1 0℃ )良好生长 ,而且有部分菌株能够在低温条件下分解利用淀粉或纤维素等...

在 1 999年北极夏季期间对楚科奇海和白令海的海洋低温微生物进行了调查。在楚科奇海 ,海洋细菌和海洋真菌的检出率分别为 1 0 0 %和 >94% ,其相应的总量一般分别为 >1 0 3 cells/cm3 和 1 0 1— 1 0 3 cells/cm3 ;在大多数站位 ,海洋细菌的总量通常都高于海洋真菌的总量 ;从表层至 1 0 m或 3 0 m深层的海水区域 ,分布有丰富的海洋微生物。调查结果还显示 ,不同站位间的海洋微生物总量存在明显差异 ,海冰的融化和海水中的盐度可能是影响这一海区海洋微生物总量的重要因素。在白令海 ,海洋细菌的检出率为 1 0 0 % ,其总量一般在 1 0 2 — 1 0 3 cells/cm3 ;海洋真菌的检出率 >84% ,其总量一般也在 1 0 2 — 1 0 3 cells/cm3 。白令海不同站位间的海洋微生物总量也存在较大的差异。调查结果证实在楚科奇海和白令海的确存在大量的低温海洋微生物 ,在所研究的海洋细菌中 ,分别有 81 %的楚科奇海细菌和 88.9%的白令海细菌能够在低温条件下 (<1 0℃ )良好生长 ,而且有部分菌株能够在低温条件下分解利用淀粉或纤维素等多糖物质以满足自身生长的营养需求。这些海洋低温菌株为进一步开发利用这些海域的海洋微生物资源提供了充足的材料

The distribution properties of total soil microbial DNA resources and the relationships between the total microbial total DNA distribution and the microbial number,biochemical activityies and other environmental factors in different vegetation soils of Tiantai mountain were studied.The developing degree of soil and the fertilizing managements take great effect upon the distribution of the total microbial total DNA resources in soils.In addition,the soil microbial number,microbial...

The distribution properties of total soil microbial DNA resources and the relationships between the total microbial total DNA distribution and the microbial number,biochemical activityies and other environmental factors in different vegetation soils of Tiantai mountain were studied.The developing degree of soil and the fertilizing managements take great effect upon the distribution of the total microbial total DNA resources in soils.In addition,the soil microbial number,microbial biomass C and soil respiration rate play obviously direct role in the distribution of the total DNA resources,the interactions of which also play obviously indirect role.The distribution of the total microbial total DNA play important role in soil pH,organic matter,total nitrogen,total phosphorus,total postassium content.

研究了天台山8种植被类型土壤微生物DNA总量分布的特性及其与土壤微生物数量、土壤生化活性和土壤其它环境因素之间的关系。土壤的发育程度等对土壤微生物DNA总量分布影响较大。此外,土壤微生物数量、微生物生物量C和土壤呼吸速率对土壤微生物DNA总量分布的直接作用和它门之间的相互作用产生的间接影响较显著。在所测的8种土壤理化因素中,土壤微生物DNA总量分布对土壤pH、有机质、全氮、全磷、全钾影响较大。

With culture-independent approach, microbial total DNA was directly extracted from Pachychalina sp. Using the total microbial DNA as template, archaeal 16S rDNAs were amplified by PCR with universal primers. Amplified products were cloned into T-vector and secondarily amplified by PCR. Then the secondarily amplified products were purified to be further characterized by termed ARDRA(amplified rDNA restriction analysis,ARDRA). According to the enzyme restriction mapping, the apparent difference...

With culture-independent approach, microbial total DNA was directly extracted from Pachychalina sp. Using the total microbial DNA as template, archaeal 16S rDNAs were amplified by PCR with universal primers. Amplified products were cloned into T-vector and secondarily amplified by PCR. Then the secondarily amplified products were purified to be further characterized by termed ARDRA(amplified rDNA restriction analysis,ARDRA). According to the enzyme restriction mapping, the apparent difference among them were disclosed. Further more eight archaeal cloned partial sequences were acquired and builded up a phylogenetic tree. In the phylogenetic tree, the eight archaea belonged to Methanogenium organophilum and Methanoplanus petrolearius, but the 16S rDNAs similarities among them and those archaea registered in RDP Database didn't excess to 90%. It means that they maybe represent some novel archaeal groups.

采用非分离培养分析方法 ,即 16SrDNA限制性酶切片段长度多态性 (ARDRA)和测序方法对南海湛江海域海绵Pachychalinasp .体内的古菌多样性进行了研究。从海绵体内直接提取古菌总DNA。以样品总DNA为模板 ,用古菌 16SrDNA通用引物进行PCR扩增获得 16SrDNA ,回收、纯化 16SrDNA产物并克隆到T Vector。进行第二次PCR扩增反应 ,且对扩增产物进行ARDRA。在古菌 16SrDNA的ARDRA图谱中 ,大多数克隆的酶切带谱上存在差异 ;随机挑选 8个克隆子进行测序 ,获得古菌 16SrDNA的部分序列 ,并对 16SrDNA序列进行聚类分析构建了系统进化树 ,结果发现海绵体内的古菌主要属于Methanogeniumorganophilum、Methanoplanuspetrolearius等古菌类。但它们与目前数据库中收录的古细菌间的相似性均不超过 90 % ,它们极有可能是一些新的古菌

 
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