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glycoprotein i gene
相关语句
  gi基因
     SEQUENCE ANALYSIS OF GLYCOPROTEIN I GENE OF MAREK′S DISEASE VIRUS CVI988
     马立克氏病病毒疫苗CVI988株囊膜糖蛋白gI基因的序列分析
短句来源
     Cloning and Expression of Glycoprotein I Gene of Marek′s Disease Virus Strain 648
     马立克氏病病毒648株囊膜糖蛋白gI基因的克隆和表达的研究
短句来源
     Cloning and Expression of Glycoprotein I Gene of Marek′s Disease Virus Strain G2
     马立克氏病病毒广西株G2囊膜糖蛋白gI基因的克隆和表达
短句来源
     Molecular Characterisation of Marek's Disease Virus Glycoprotein I Gene
     马立克氏病病毒囊膜糖蛋白gI基因的分子生物学特性
短句来源
     Glycoprotein I Gene(gI) was amplified from genomic DNA of Marek's disease virus(MDV) 648 strain by polymerase chain reaction(PCR).
     通过聚合酶链式反应 (PCR) ,扩增出马立克氏病病毒特超强毒 (vv +MDV) 648株囊膜糖蛋白gI基因 ,并将该基因按正确的阅读框 (ORF)克隆到表达性载体质粒pGEX 6P 1中谷胱甘肽转移酶 (GST)基因的下游。
短句来源
  糖蛋白i基因
     Expression and purification of varicella-zoster virus glycoprotein I gene in insect cells
     水痘-带状疱疹病毒糖蛋白I基因在昆虫细胞中的表达及纯化
短句来源
  “glycoprotein i gene”译为未确定词的双语例句
     Comparing DNA Sequences in Glycoprotein I Gene of Marek's Disease Viruses of Different Pathotypes
     特超强毒型马立克病病毒囊膜糖蛋白I基因序列及与其它致病型毒株的比较
短句来源
     The glycoprotein I gene (gI) of vv +MDV strain 648A was amplified by PCR, cloned and sequenced, its DNA and amino acid sequences were compared to published sequences of vMDV strain GA and vvMDV strain RB1B. The results indicated that the gI sequence of 648A was identical to the published 761 bases of 5′ side of ORF of strain RBIB, but there were 8 bases and then 7 amino acids different between GA and 648A strains.
     特超强毒型 6 48A株马立克病病毒 (MDV)的囊膜糖蛋白I(gI)基因经PCR扩增后克隆进pUC18质粒载体 ,并对其ORF完成了DNA测序。 与已发表的其它致病型毒株的糖蛋白I的DNA和氨基酸序列比较表明 ,6 48A株的 gI基因序列与超强毒RBIB株已发表的ORF 5′端 76 1个碱基完全相同。
短句来源
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The glycoprotein I gene (gI) of vv +MDV strain 648A was amplified by PCR, cloned and sequenced, its DNA and amino acid sequences were compared to published sequences of vMDV strain GA and vvMDV strain RB1B. The results indicated that the gI sequence of 648A was identical to the published 761 bases of 5′ side of ORF of strain RBIB, but there were 8 bases and then 7 amino acids different between GA and 648A strains. All these base changes located at the 5′ end of gI, about the one third of the 3′ end...

The glycoprotein I gene (gI) of vv +MDV strain 648A was amplified by PCR, cloned and sequenced, its DNA and amino acid sequences were compared to published sequences of vMDV strain GA and vvMDV strain RB1B. The results indicated that the gI sequence of 648A was identical to the published 761 bases of 5′ side of ORF of strain RBIB, but there were 8 bases and then 7 amino acids different between GA and 648A strains. All these base changes located at the 5′ end of gI, about the one third of the 3′ end was identical in different pathotypes.

特超强毒型 6 48A株马立克病病毒 (MDV)的囊膜糖蛋白I(gI)基因经PCR扩增后克隆进pUC18质粒载体 ,并对其ORF完成了DNA测序。与已发表的其它致病型毒株的糖蛋白I的DNA和氨基酸序列比较表明 ,6 48A株的 gI基因序列与超强毒RBIB株已发表的ORF 5′端 76 1个碱基完全相同。但是在该基因中完整ORF的 10 6 8个碱基中 ,6 48A株与强毒GA株间有 8个碱基变异并导致 7个氨基酸的变化 ,且这一变化主要发生在该基因的 5′端 ,其 3′端三分之一完全相同

Glycoprotein I Gene(gI) was amplified from genomic DNA of Marek's disease virus(MDV) 648 strain by polymerase chain reaction(PCR).PCR product was cloned into pGEX\|6p\|1 according to the right open reading frame(ORF).The expression of GST\|gI fusion protein was studied in detail on many factors including temperature,timing and IPTG.The curve for OD 600 and the growing time of the recombinant bacteria is also established.,which is helpful to find the optimal inducing time.GST\|gI fusion protein was...

Glycoprotein I Gene(gI) was amplified from genomic DNA of Marek's disease virus(MDV) 648 strain by polymerase chain reaction(PCR).PCR product was cloned into pGEX\|6p\|1 according to the right open reading frame(ORF).The expression of GST\|gI fusion protein was studied in detail on many factors including temperature,timing and IPTG.The curve for OD 600 and the growing time of the recombinant bacteria is also established.,which is helpful to find the optimal inducing time.GST\|gI fusion protein was identified by SDS\|PAGE and Western\|blotting., and the result shows that the best concentration of IPTG is 0.2~0.5mmol/L and inducing time have great effects on expression while temperature has little.The fusion protein was injected into mouse five times to identify its antigenicity and the result is positive in indirect fluorescent assay IFA.

通过聚合酶链式反应 (PCR) ,扩增出马立克氏病病毒特超强毒 (vv +MDV) 648株囊膜糖蛋白gI基因 ,并将该基因按正确的阅读框 (ORF)克隆到表达性载体质粒pGEX 6P 1中谷胱甘肽转移酶 (GST)基因的下游。重组质粒 (pGEX gI)经氯化钙转化宿主菌BL2 1 ;通过建立重组菌生长时间与OD60 0 值间的关系曲线 ,以及对诱导时间、诱导温度、IPTG浓度等条件的摸索 ,根据聚丙烯酰胺凝胶电泳 (SDS PAGE)判定GST gI融合蛋白的最佳表达条件 ,并经蛋白质印迹试验 (WesternBlotting)对表达产物进行了验证。将表达产物免疫小鼠 ,所得抗血清能与MDV感染的鸡胚成纤维细胞 (CEF)在间接免疫荧光试验 (IFA)中 ,呈较强的细胞膜荧光着色。实验结果表明 :IPTG的最佳浓度为 0 2~ 0 5mmol L ;最适的诱导时期为重组菌生长对数中期 ;温度对表达几乎没有影响。pGEX载体表达的融合蛋白至少保留了天然蛋白的部分抗原性

 
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