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gene gm
相关语句
  基因gm
     Transformation of Stress Resistance Related Gene GM and FMDV Structure Protein Gene P1 into Soybean
     抗逆相关基因GM和口蹄疫结构蛋白全长P1基因转化大豆的研究
短句来源
     Application of the New Gene Gm6 Against Rice Gall Midge in Resistant Breeding Through PCR Based-marker Aided Selection
     抗稻瘿蚊新基因Gm6在分子标记抗性育种中的应用
短句来源
     (2) fine mapping of the resistance gene Gm6 in rice germplasm `Daqiuqi' from China using RAPD and SSR markers;
     (2)分别用RAPD和微卫星SSR技术对来源于我国的水稻资源大秋其的抗亚洲稻瘿蚊基因Gm6进行精细定位;
短句来源
     On the basis of fine mapping of Gm6 from resistant rice variety Daqiuqi, application of position-specific microsatellite maker closely linked to gene Gm6 in rice resistant breeding.
     在对源于我国抗稻瘿蚊资源大秋其的抗稻瘿蚊基因Gm6精细定位的基础上,利用与Gm6连锁的位置特异性微卫星(position-specificmicrosatellite,PSM)标记进行分子标记辅助抗性育种。
短句来源
     The research results of selecting and breeding resistant varieties and lines using marker aided selection (MAS) on the basis of the molecular markers linked to gene Gm6 against rice gall midge (Orseolia oryzae Wood-Mason) were reported.
     在发现与抗稻瘿蚊的基因Gm6紧密连锁的分子标记的基础上 ,应用分子标记辅助选育 (markeraidedse lection ,MAS)方法 ,成功地选育出抗稻瘿蚊的品系。
短句来源
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  gm基因
     The stress resistance-related gene GM and Foot and Mouth disease virus structure protein gene P1 were transformed into immature cotyledon and somatic embryogenesis of soybean via Agrobacterium-mediated and particle bombardment. The regenerated plantlets were examined by PCR and PCR-southern blot. The results indicated that target gene had been transformed into soybean, and the factors affecting genetic transformation were investigated.
     以10 余个东北地区主栽的大豆品种未成熟子叶和体细胞胚为受体材料,通过农杆菌介导和基因枪法,将抗逆相关GM 基因和编码口蹄疫病毒结构蛋白全长基因P1 导入大豆基因组中,获得了抗性植株,经PCR、PCR-southern 杂交和基因组DNA 点杂交等分子生物学方法检测,证明目的基因已整合到大豆基因组中。
短句来源
  “gene gm”译为未确定词的双语例句
     (3) establishment of the breeding technique for new resistant cultivars using MAS based on the closely-linked STS and SSR markers to the gene Gm6;
     (3)分别用与Gm6基因紧密连锁的STS分子标记和SSR标记,建立了分子标记辅助选择(MAS)方法选育抗稻瘿蚊新品种的技术体系;
短句来源
     Application of PSM maker linked to gene Gm6 in rice resistant breeding
     与Gm6基因连锁的PSM标记在水稻抗稻瘿蚊育种中的应用
短句来源
     International Rice Research Institute evaluating method on resistant of rice varieties to the rice gall midge (GM) and six differential varieties including Leuang 152(with gene Gm2), OB677 (with gene gm4), IET2911(with gene Gm2), W1263(with Gm1), Ptb21, Muey Nawng62M were applied to monitor the biotypes of the GM in Guangxi.
     采用国际稻瘿蚊抗性鉴定方法和 6个鉴别品种 Leuang1 5 2 (含 Gm2 )、OB677(含 gm4)、IET2 91 1(含 Gm2 )、W1 2 63 (含 Gm1 )、Ptb2 1和 Muey Nawng62 M,对广西稻区代表点的稻瘿蚊田间种群生物型进行测定 ,结果表明广西有 2个不同的生物型 ,即中国 型和中国 型。
短句来源
     (2) fusion gene GM CSF HV\-2 was amplified by PCR.
     (2)利用 P C R介导,扩增出了融合基因片段h G M C S F H V2。
短句来源
     2 Two segregating populations derived from two crosses between two susceptible varieties, 'G2417-2-1' and 'G3004-4', and the resistant variety 'Kangwenqingzhan' were used for maping and physical mapping of a gall midge resistance gene Gm6 in rice.
     共发展了 198个PSM标记 ,有 10 5个标记的基序为GA/CT重复 ,占 5 3 3% ,绝大多数标记 (98 0 % )的重复序列长度在 2 0bp以上。
短句来源
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  gene gm
Hence SSR markers RM219 and RM444, flanking the gene Gm1, and RM317, RM241 along with the SCAR marker F8, flanking the gene Gm2, were selected for this study.
      
The gene Gm was derived from Solanum gourlayi, whereas, Solanum megistacrolobum is the source of the gene Rm.
      
Identification of flanking SSR markers for a major rice gall midge resistance gene Gm1 and their validation
      
A set of PCR primers specific to an RFLP marker, previously identified to be linked to another gall midge resistance gene Gm2, also amplified a 1.5-kb (F8LB) fragment that is linked to Gm7.
      
The Chinese rice cultivar Duokang #1 carries a single dominant gene Gm-6(t) that confers resistance to the four biotypes of Asian rice gall midge (Orseolia oryzae Wood-Mason) known in China.
      
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Objective: To make the vector of GM CSF/Fcγ\-2 fusion protein construct. Mathods: Genes for human GM CSF and Fc domain of human IgG2 were joined by PCR, hGM CSF HV\-2 fusion genes were constructed; and the fusion genes were cloned into the eukaryotic expression plasmid pRc/CMV2. Results: The results of agarose electrophoresis indicated that 1.3 kb long fusion gene hGM CSF HV\-2 was amplified by PCR; the map of restriction enzyme analysis indicated that the vector of...

Objective: To make the vector of GM CSF/Fcγ\-2 fusion protein construct. Mathods: Genes for human GM CSF and Fc domain of human IgG2 were joined by PCR, hGM CSF HV\-2 fusion genes were constructed; and the fusion genes were cloned into the eukaryotic expression plasmid pRc/CMV2. Results: The results of agarose electrophoresis indicated that 1.3 kb long fusion gene hGM CSF HV\-2 was amplified by PCR; the map of restriction enzyme analysis indicated that the vector of GM CSF/Fcγ\-2 fusion protein was constructed successfully. Conclusion: (1) hGM CSF cDNA and Fc domain of human IgG2 were joned by PCR by using PCD hGM CSF and PSV VNP HV\-2 plasmid. (2) fusion gene GM CSF HV\-2 was amplified by PCR. (3) the vector of the eukaryotic expression pRc/CMV2/hGM CSF HV\-2 was constructed.

目的:构建 G M C S F/ Fcγ2 融合蛋白载体。方法:利用 P C R技术,将人 G M C S F基因与人免疫球蛋白 Ig G2 的 Fc 段基因联接,构建融合基因h G M C S F H V2;并将此片段定向克隆到真核表达质粒p Rc/ C M V2 载体。结果:琼脂糖凝胶电泳结果显示 P C R扩增出了 1.3 kb 的融合基因片段;限制性内切酶图谱分析,成功地完成了 G M  C S F/ Fcγ2 融合蛋白载体的构建。结论:(1)经 P C R 扩增技术由质粒 P C Dh G M C S F和 P S V V N P H V2 分别扩增到了所需的h G M C S F c D N A 片段和 Ig G2 从绞链区到 C H3 的基因组 D N A 片段。(2)利用 P C R介导,扩增出了融合基因片段h G M C S F H V2。(3)构建了真核细胞表达载体p Rc/ C M V2/h G M C S F H V2 。

The resistance inheritance background of Duokang 1 to rice gall midge ( Orseolia oryzae (Wood-Mason))was explored, the resistance of filial generations F1 , F2, F3 and T1 of Duokang 1 and sensitive/resistant (possessing different resistant genes) varieties was tested, the proportions of resistance segregation were detemined, and the resistance inheritance mode of Duokang 1 as well as the allelisms among Duokang 1 and other representative resistant sources were analyzed. The results showed that the resistance...

The resistance inheritance background of Duokang 1 to rice gall midge ( Orseolia oryzae (Wood-Mason))was explored, the resistance of filial generations F1 , F2, F3 and T1 of Duokang 1 and sensitive/resistant (possessing different resistant genes) varieties was tested, the proportions of resistance segregation were detemined, and the resistance inheritance mode of Duokang 1 as well as the allelisms among Duokang 1 and other representative resistant sources were analyzed. The results showed that the resistance of Duokang 1 to Orseolia oryzae is controlled by a single dominant gene Gm-6, which is nonallelic to those of Leuang 152, BG404-1 and OB677, but linked with that of Yangshanzhan, and different from gm-3 in ARC 5984. The application perspective of Gm-6 was discussed.

为了明确抗源大秋其的衍生品系多抗1号对稻瘿蚊抗性的遗传背景,测定了多抗1号与感稻瘿蚊品种及不同抗稻瘿蚊基因抗源的杂交F_1、F_2、F_3和T_1的抗性,分析抗性分离比例,研究多抗1号对稻瘿蚊抗性遗传方式及其与一些有代表性抗源抗性基因的等位性关系。结果表明,多抗1号对广东稻瘿蚊的抗性由显性主基因控制。它的抗稻瘿蚊基因Gm-6与Leuang152、BG404-1、OB677的抗稻瘿蚊基因不等位,与羊山占的抗稻瘿蚊基因连锁,与ARC5984的抗稻瘿蚊基因gm-3是不同的抗稻瘿蚊基因。

Rice is the important crop for human consumption, providing staple food for about half the world's population. The construction of rice microsatellite map will contribute greatly to genetic research and molecular breeding. In this study, PSM (position specific microsatellite) markers were developed in rice, and then an integrated microsatellite map was constructed by using these PSM markers and also some other published RM markers. Two insect resistant genes viz. Gm6 for gall midge and Bph3...

Rice is the important crop for human consumption, providing staple food for about half the world's population. The construction of rice microsatellite map will contribute greatly to genetic research and molecular breeding. In this study, PSM (position specific microsatellite) markers were developed in rice, and then an integrated microsatellite map was constructed by using these PSM markers and also some other published RM markers. Two insect resistant genes viz. Gm6 for gall midge and Bph3 for brown planthopper were mapped using some SSR markers and one STS marker in rice. The main results were as follows: 1. Based on published sequences on the websites, position specific microsatellite primers were designed and an integrated microsatellite map was constructed in silico. A total of 198 primer pairs were designed, among which 105 primer pairs (53 3%) whose motif types were poly(GA/CT)n. The majority (98%) of developed markers had SSRs20bp in length. Previously mapped SSR markers and newly developed PSM markers were integrated into RGP map and finally a 718 SSR marker genetic map covering 1527 2 cM was constructed. The integrated map was estimated to have one SSR marker at every 2 13 cM on an average. Chromosome 1 has a highest average density of SSR markers i e one at every 1 73 cM, whereas chromosome 11 has a lowest average density of SSR markers i e one at 3 32 cM Compared with newly developed IRMI map with high density of SSR markers, our developed map has more uniform distribution of makers with 3 genetic gaps10 cM and 5 genetic gaps between 8 cM and 10 cM, whereas there are 17 and 12 gaps on IRMI map, respectively. 2 Two segregating populations derived from two crosses between two susceptible varieties, 'G2417-2-1' and 'G3004-4', and the resistant variety 'Kangwenqingzhan' were used for maping and physical mapping of a gall midge resistance gene Gm6 in rice. Based on linkage analysis of these two populations consisting of 239 and 243 F 2 individuals, respectively, Gm6 was mapped on the long arm of chromosome 4 by using two SSR markers (RM348 and RM255) and one STS marker (RG476/ScaⅠ). Position specific microsatellite markers were developed for fine mapping of Gm6 gene on the basis of published sequences on the websites. The results showed that Gm6 was mapped between PSM112 and PSM114 at the genetic interval of 0 4 cM, whereas three additional markers PSM101, PSM106, and PSM115 showed no recombination with Gm6 . Two overlapping BAC clones AL606660 and AL606645 covering Gm6 gene were used for physical mapping. With the known physical information of all the markers in the two overlapping BAC clones, a sequence of ~158kb was defined as the Gm6 containing region. 3 An F 2 mapping population derived from a cross between the resistant variety 'RH' and the susceptible variety 'Qishiruanzhan' was used to map Bph3 gene that confers resistance to brown planthopper of rice. Based on the phenotypic results of F 3 lines, 27 F 2 individuals selected from the population consisting of 237 F 2 individuals were used for molecular mapping of Bph3 gene. Through linkage analysis of polymorphic SSR markers of chromosome 4 with Bph3 gene, two SSR markers (RM261 and RM119) were found to have distances of 8 0 cM and 8 0 cM with Bph3 , respectively. For further analysis, PSM (position specific microsatellite) markers were developed in the region between RM261 and RM119. Two polymorphic PSM markers (PSM323 and PSM194) were mapped with Bph3 gene of 8 0cM and 3 8cM distances, respectively. Therefore, Bph3 gene was mapped between PSM323 and PSM194 on the long arm of chromosome 4.

水稻是世界上最重要的粮食作物之一 ,为世界近一半人口提供食物来源。水稻微卫星图谱的构建有利于遗传基础的研究及分子育种。本研究通过发展位置特异性微卫星标记 ,对微卫星标记进行了图谱的整合 ,并利用微卫星等标记对水稻抗稻瘿蚊基因Gm6和抗褐飞虱基因Bph3进行了分子定位。主要研究结果如下 :1、利用网上公布的序列信息进行位置特异性微卫星引物的设计和微卫星图谱的整合。共发展了 198个PSM标记 ,有 10 5个标记的基序为GA/CT重复 ,占 5 3 3% ,绝大多数标记 (98 0 % )的重复序列长度在 2 0bp以上。将原有微卫星标记和本实验发展的PSM标记整合到RGP遗传图谱上 ,整合后总的SSR标记数为 718个 ,新图谱的遗传距离总长度为 15 2 7 2cM ,平均标记密度为每 2 13cM有一个SSR标记 ,其中第 1染色体的标记最密 (1 73cM /个 ) ,而第 11染色体的标记密度最低 (3 32cM /个 )。将本研究发展的微卫星图谱与IRMI发表的高密度微卫星进行了比较 ,结果显示本研究发展的微卫星图谱标记分布比较均匀 ,只有 3个区域标记间遗传间距在 10cM以上 ,5个区域在 8...

水稻是世界上最重要的粮食作物之一 ,为世界近一半人口提供食物来源。水稻微卫星图谱的构建有利于遗传基础的研究及分子育种。本研究通过发展位置特异性微卫星标记 ,对微卫星标记进行了图谱的整合 ,并利用微卫星等标记对水稻抗稻瘿蚊基因Gm6和抗褐飞虱基因Bph3进行了分子定位。主要研究结果如下 :1、利用网上公布的序列信息进行位置特异性微卫星引物的设计和微卫星图谱的整合。共发展了 198个PSM标记 ,有 10 5个标记的基序为GA/CT重复 ,占 5 3 3% ,绝大多数标记 (98 0 % )的重复序列长度在 2 0bp以上。将原有微卫星标记和本实验发展的PSM标记整合到RGP遗传图谱上 ,整合后总的SSR标记数为 718个 ,新图谱的遗传距离总长度为 15 2 7 2cM ,平均标记密度为每 2 13cM有一个SSR标记 ,其中第 1染色体的标记最密 (1 73cM /个 ) ,而第 11染色体的标记密度最低 (3 32cM /个 )。将本研究发展的微卫星图谱与IRMI发表的高密度微卫星进行了比较 ,结果显示本研究发展的微卫星图谱标记分布比较均匀 ,只有 3个区域标记间遗传间距在 10cM以上 ,5个区域在 8- 10cM ,而IRMI微卫星图谱分别有 17和 12个。2、采用G2 4 17- 2 - 1×抗蚊青占 (2 39株 )和G30 0 4 - 4×抗蚊青占 (2 4 3株 )两个F2 作图群体对水稻抗稻瘿蚊进行了分?

 
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