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h glycoprotein gene
相关语句
  h糖蛋白基因
     Construction of prokaryotic vector and expression of H glycoprotein gene of peste des petits ruminants virus
     小反刍兽疫病毒H糖蛋白基因原核表达载体的构建及表达
短句来源
  “h glycoprotein gene”译为未确定词的双语例句
     The H glycoprotein gene of peste des petits ruminants virus(PPRV),which was synthesized artificially according to the Indian vaccine,was sub-cloned from pUC18-H,and inserted into pBAD/Thio-TOPO vector.
     参照GenBank中小反刍兽疫病毒(PPRV)H抗原基因序列,人工合成了PPRV H基因,将其克隆至pUC18-T质粒中,转化E.
短句来源
     Sequence analysis confirmed that the H glycoprotein gene was inserted correctly.
     coliTOP10感受态细胞,核苷酸序列分析证实,成功构建了PPRV H基因重组表达载体。
短句来源
  相似匹配句对
     Advances in Inhibitor of P-Glycoprotein
     P-糖蛋白抑制剂的研究进展
短句来源
     P-glycoprotein and intractable epilepsy
     P-糖蛋白与难治性癫痫的关系
短句来源
     The construction and function of Glycoprotein
     糖蛋白的结构与功能
短句来源
     P-GLYCOPROTEIN AND VOLUME REGULATION
     P-gp和细胞容积调节
短句来源
     The gene of the glycoprotein g13 of EHV- I was sequenced.
     本文分析了其膜糖蛋白g13基因的碱基序列,并由此推导其相应的氨基酸序列。
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The H glycoprotein gene of peste des petits ruminants virus(PPRV),which was synthesized artificially according to the Indian vaccine,was sub-cloned from pUC18-H,and inserted into pBAD/Thio-TOPO vector.The recombinant plasmid was identified by PCR.Sequence analysis confirmed that the H glycoprotein gene was inserted correctly.SDS-PAGE analysis revealed that the H protein gene was expressed at high level in Escherichia coli TOP10.The expressed fusion protein,which made up 10% of total...

The H glycoprotein gene of peste des petits ruminants virus(PPRV),which was synthesized artificially according to the Indian vaccine,was sub-cloned from pUC18-H,and inserted into pBAD/Thio-TOPO vector.The recombinant plasmid was identified by PCR.Sequence analysis confirmed that the H glycoprotein gene was inserted correctly.SDS-PAGE analysis revealed that the H protein gene was expressed at high level in Escherichia coli TOP10.The expressed fusion protein,which made up 10% of total bacteria protein after being induced with 0.2g/L of L-arabinose for 5 hours,was approximately 83ku in molecular weight.In Western-blotting test,the protein can specifically react with the PPRV H monoclonal antibody,indicating that prokaryotic expression of PPRV H gene can produce H glycoprotein.

参照GenBank中小反刍兽疫病毒(PPRV)H抗原基因序列,人工合成了PPRV H基因,将其克隆至pUC18-T质粒中,转化E.coliJM109感受态细胞,构建并选择PPRV H基因克隆重组质粒,经核苷酸序列分析正确,将其克隆至pBAD/Thio-TOPO载体中,转化E.coliTOP10感受态细胞,核苷酸序列分析证实,成功构建了PPRV H基因重组表达载体。经不同浓度L-阿拉伯糖诱导,可稳定、高效地表达PPRV H抗原。SDS-PAGE分析结果表明,用终浓度为0.2 g/L的L-阿拉伯糖诱导5 h的表达量最高,表达蛋白为分子质量约83 ku的融合蛋白;经薄层扫描分析,其表达产量约占菌体总蛋白的10%。Western-blotting检测表明,诱导的蛋白能与PPRV H蛋白单抗发生特异性反应,说明表达的融合蛋白中含有PPRV H糖蛋白抗原。

 
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