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endonucleases
相关语句
  核酸内切酶
     Plasmid pBR322 has been enlarged twice in vitro and the restriction enzyme mappings have been done for 4 newly constructed plasmids (pBW5,pBW7,pBW5-2 and pBW5 -8) by the related restriction endonucleases.
     在体外对质粒pBR322进行了两次扩建,并作出新建四种质粒(pBW5,pBW7,pBW5-2和pBW5-8)的有关限制性核酸内切酶图谱。
短句来源
     Methods:The recombinant plasmid pGEM-T-E7 of HPV16E7 local strain was enzyme-digested with restriction endonucleases to get E7 gene subclone. E7 was subcloned into the eukaryotic expression vector pcDNA3.1(-) . The CHO cells were transfected with pcDNA-E7. E7 protein expression was identified by immunofluorescence assay.
     方法从先期构建的地方株HPV16E7基因重组质粒pGEM-T-E7经限制性核酸内切酶酶切,获得E7基因亚克隆到真核表达载体pcDNA3.1(-)中,构建地方株pcDNA-E7基因疫苗,转染CHO细胞,通过IFA试验验证E7蛋白的表达。
短句来源
     The activities of restriction endonucleases were found in 10 out of 66 strains of Bacillus isolated and examined in our country.
     我们从66株国内收集的芽孢杆菌中筛选出10株菌株,它们含有限制性核酸内切酶活力。
短句来源
     Beta1-toxin gene was amplified from chromosomal DNA of Clostridium perfringens type C by Polymerase Chain Reaction (PCR),PCR products were cleaved with restriction endonucleases BamHI and EcoRI,then the 0.95kb gene fragments were recovered and inserted into the BamHI/EcoRI site of pET-28c vector.
     利用PCR技术 ,从C型产气荚膜梭菌染色体DNA中扩增出 β1 毒素基因 ,然后用限制性核酸内切酶BamHI和EcoRI对其进行双酶切处理 ,回收 0 .95kb的β1 毒素基因片段 ,最后将其定向克隆在事先经同样内切酶处理的载体pET - 2 8c中相应位点上 ,转化至受体菌BL2 1(DE3)中。
短句来源
     The fragment is 184 base pairs long and contains the restriction endonucleases sites of Xba Ⅰ, HaeⅢ, BstNI, and HinfI.
     其中包括XbaⅠ、HaeⅢ、B_(st)NI、HinfI限制性核酸内切酶位点。
短句来源
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  “endonucleases”译为未确定词的双语例句
     The mitochondrial DNA (mtDNA) of Megelobrama amblycephala were digested by 11 restriction endonucleases: BamH Ⅰ, Bgl Ⅰ, Bgl Ⅱ, EcoR Ⅰ, Hpa Ⅰ, Kpn Ⅰ, Pst Ⅰ, Sac Ⅰ,Sal Ⅰ, Xba Ⅰ, Xho Ⅰ into different fragment: 2, 3, 2, 3, 3, 1, 0, 1, 0, 0, 0 respeCtively.
     用BamHⅠ,BglⅠ,BglⅡ,EcoRⅠ,HpaⅠ,KpnⅠ,PstⅠ,SacⅠSalⅠ,XbaⅠ,和XhoⅠ11种限制性内切酶对团头鲂(MegalobramaamblycephalaYih)的线粒体DNA(mtDNA)进行了单酶切,其切点数依次为2,3,2,3,3,1、0,1,0,0和0;
短句来源
     Methods Using recombinant plasmid pGEM-T-IκBα as template, the IκBα gene cDNA with BamHⅠand HindⅢ sites was amplified by PCR method and then inserted into the prokaryotic expression vector pET-32a(+) after been digested by corresponding endonucleases.
     方法 以重组质粒pGEM T IκBα为模板 ,利用PCR方法扩增出带有BamHⅠ和HindⅢ酶切位点的人IκBα基因cDNA ,经相应酶切后插入原核表达载体pET 32a(+)。
短句来源
     The structure of pTrc99Am HPV16 L1 had been confirmed by restriction endonucleases digestion.
     通过限制性内切酶分析验证了pTrc99AmHPV16L1的结构。
短句来源
     Results Endonucleases digestion confirmed that IκBα gene cDNA has been inserted into the prokaryotic expression vector pET-32a(+).
     结果 酶切鉴定证实人IκBα基因cDNA已插入原核表达载体pET 32a(+)。
短句来源
     Methods: 23 samples that had been proved to be type 1b and type 2a, respectively, were analyzed and typed by selecting with 13 restriction endonucleases : Hpa Ⅱ 、 Cfr 10Ⅰ、 Alu Ⅰ、 Hha Ⅰ、 Hae Ⅲ、 Apa Ⅰ、 Ava Ⅱ、 Taq Ⅰ、 Bst NⅠ、 Sau 3AⅠ、 Stu Ⅰ、 Ba Ⅱ、 and Sma Ⅰ.
     方法 :选用了HpaⅡ、Cfr10Ⅰ、AluⅠ、HhaⅠ、HaeⅢ、ApaⅠ、AvaⅡ、TaqⅠ、BstNⅠ、Sau 3AⅠ、StuⅠ、BalⅠ、SmaⅠ等 13种限制性内切酶 ,对已知2 3例 1b型和 17例 2a型样品进行酶切分析和验证。
短句来源
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  相似匹配句对
     THE RESTRICTION ENDONUCLEASES FROM BACILLUS
     芽孢杆菌中的限制性核酸内切酶
短句来源
     Analysis of the Chromosome Banding with Restriction Endonucleases
     用限制性内切酶诱导染色体显带的分析
短句来源
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  endonucleases
Digestion of amplified products of each nematode isolate with five restriction endonucleases revealed the following results: 1) Dra I digestion of the internal transcribed spacer (ITS) products of B.
      
The restriction endonucleases Dra I and Sal I could be used to identify B.
      
After identification by sequencing and restriction endonucleases, the recombined vector was transformed into BL-21 (DE3) E.
      
Restriction endonucleases from various bacterial strains displaying an ice-nucleating activity
      
Six strains containing site-specific endonucleases II were selected from a collection of 45 ice-nucleating bacterial strains isolated from rhizosphere of plants growing in various geographical regions.
      
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By means of methylated albumin kieselguhr (MAK) column, we have isolated and purified plasmids pBR322, ColE1, pSC101, pCR1 and baoteriophage λ. DNA. They have been characterized by analysis with the EcoR1 restriction endonuclease, electrophoresis of DNAs on agarose gel, and transformation by R-faotors. The results indicate that plasmids and λ DNA, prepared on MAK column, are pure, and can be used as vectors for construction of recombinant DNA, and as substrates for the restriction endonucleases.

用甲酯化白蛋白硅藻土(MAK)柱层析方法分离纯化的质粒pBR322,ColE_1,pSC101,pCR1和λDNA,经EcoR_1限制性内切酶作用,琼脂糖凝胶电泳分析,抗药因子转化,结果表明这些材料可以用作DNA重组体的载体和限制性内切酶的底物。

By means of methylated albumin kieselguhr (MAK) column, we have isolated andpurified plasmids pBR322, ColE1, pSC101, pCR1 and bacteriophage λ DNA. Theyhave been characterized by analysis with the EcoR1 restriction endonuclease, electro-phoresis of DNAs on agarose gel, and transformation by R-factors. The resultsindicate that plasmids and λ DNA, prepared on MAK column, are pure, and can beused as vectors for construction of recombinant DNA, and as substrates for the restriction endonucleases.

用甲酯化白蛋白硅藻土(MAK)柱层析方法分离纯化的质粒pBR322,ColE_1,pSC101,pCRl 和λDNA,经EcoR_1限制性内切酶作用,琼脂糖凝胶电泳分析,抗药因子转化,结果表明这些材料可以用作DNA 重组体的载体和限制性内切酶的底物。

Pig mitochondrial DNA has been digested with BamI, BglI, EcoRI and PstI and the molecular weights of the fragments have been determined. BamI cleaves pig mtDNA at five points yielding five fragments (BamA through E), digestion with BglI produces three fragments (BglA through C),cleavage with EcoRI generates three fragments (EcoA through C), and electrophoresis after treatment with PstI yields four bands(PstA through D).The three EcoRI fragments were ordered with respect to the D-loop by gel electrophoretic and...

Pig mitochondrial DNA has been digested with BamI, BglI, EcoRI and PstI and the molecular weights of the fragments have been determined. BamI cleaves pig mtDNA at five points yielding five fragments (BamA through E), digestion with BglI produces three fragments (BglA through C),cleavage with EcoRI generates three fragments (EcoA through C), and electrophoresis after treatment with PstI yields four bands(PstA through D).The three EcoRI fragments were ordered with respect to the D-loop by gel electrophoretic and electron microscopic analysis of partially digested products and alignment of partially or completely digested forms. Based on analysis of a set of fragments generated by reciprocal double digestions with these restriction endonucleases, BamII, BglI and PstI cleavage sites were located on the map with EcoRI sites as reference points. The molecular weight of pig mtDNA is 10.40×10~6 daltons, about 15.76 kilobase pairs.

猪肝线粒体DNA经限制性内切酶BamHI、BglI、EcoRI和PstI水解分别切成5、3、3和4个片段,对这些片段的分子量进行了测定。EcoRI片段的顺序是以复制位移环(D-环)为基准通过部分水解产物的电泳和电镜分析确定的。BamHI、BglI和PstI的切割位点则根据双酶水解产物的分析,参照EcoRI位点进行定位,从而得到了由15个片段组成的物理图谱。猪肝线粒体DNA的分子量为10.40×10~6道尔顿,有15.76千碱基对。

 
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