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lectin-binding capacity
相关语句
  相似匹配句对
     Lectin Research in China
     中国的来克丁研究
短句来源
     Studies on Lectin of Pseudomonas Aeruginose
     绿脓杆菌凝集素(Lectin)的研究:Ⅰ凝集素的产生与对上皮细胞的粘附和蛋白酶分泌的关系
短句来源
     Mammalian C-Type Lectin Superfamily.
     哺乳类C型凝集素超级家族
短句来源
     Mannan-Binding Lectin and Infectious Diseases
     甘露糖结合凝集素与感染性疾病
短句来源
     Sugar Binding Activity of Lectin Fragments
     凝集素碎片的糖结合活性
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  lectin-binding capacity
The lectin-binding capacity of cartilaginous ground substance was found to be low, as was expected on account of the few free sugar residues of GAGs.
      


The effect of treating human monocytoid J6-2 leukemic cell s with cell differentiation inducers on the lectin binding capacities and on the incorporation of [ ̄125I]UdR into J6-2 cells was studied.The lectins used were BSA,ConA,DBA,LCA,PNA,PSA,RCA,SBA,SJA,UEA,and WGA.The results reveal that after 6-day treatment with GM_3(concentration,50μmol/L, the binding capacity( BC )of J6-2 cells for UEA increased(-→+), that for BSA decreased(++→-),while those for others rema- inded unchanged;after 6-day treatment...

The effect of treating human monocytoid J6-2 leukemic cell s with cell differentiation inducers on the lectin binding capacities and on the incorporation of [ ̄125I]UdR into J6-2 cells was studied.The lectins used were BSA,ConA,DBA,LCA,PNA,PSA,RCA,SBA,SJA,UEA,and WGA.The results reveal that after 6-day treatment with GM_3(concentration,50μmol/L, the binding capacity( BC )of J6-2 cells for UEA increased(-→+), that for BSA decreased(++→-),while those for others rema- inded unchanged;after 6-day treatment with DMSO (1.2%),the BC for UEA(-→+)and PNA(-→++)increased,those for BSA(++→-)and for PSA (++→+)decreased;after 3-day treatment with TPA(10ng/ml),the BC for UEA (-→+),SBA(-→+)and SJA (-→++)increased, while those for BSA ( ++→+),RCA (++→+), and WGA(++→-)decreased.The BC for DBA (-),LCA ( ++ ),and ConA(++)remained unchanged to treatments with all 3 differentiation inducers.Discussion was directed to the change of carbohydrate structure with respect to change of lectin binding capacity.The results also reveal that after treatment of J6-2 cells with the above mentioned 3 differentiation inducers,the incorporation of[ ̄125I]UdR into J6-2 cells all decreased.This indicates that the DNA synthesis of J6-2 cells was inhibited by the treatment of the differentiation inducers used in this study.

将J6-2细胞分别用分化诱导剂GM_3(50μmol/L)、DMSO(1.2%)处理6天、TPA(10ng/ml)处理3天,观察它们对11种凝集素结合反应的影响。结果表明,经GM_3处理后,J6-2细胞对凝集素结合反应增强的只有UEA(-至+),变弱的只有BSA(++至-),其余的都无变化。经DMSO处理后,结合反应变强的有UEA(-至+)及PNA(-至++),变弱的有BSA(++至-)及PSA(++至+)。经TPA处理后,结合反应变弱的有BSA(++至+)、WGA(++至-)及RCA(++至+),变强的有UEA(-至+)、SBA(-至+)及SJA(-至++)。对3种分化诱导剂处理后都无变化的有DBA(-)、LCA(++)及ConA(++)。本文还观察到3种分化诱导剂都能抑制[ ̄125I]UdR向J6-2细胞的掺入。

The possible role of lectin on the recognition between legume and rhizobium was discussed.The Stylosanthes gracilis lectin(SGL) has been extracted with ammonium sulfate and purified by chromatography on DEAE 32 column and gel filtration on Sephadex G 150 SGL exhibits one single band of molecular weight about 24 kD on SDS PAGE,while SGL's molecular weight is 45 kD estimated by gel filtration.It indicates that the lectin molecular contains two identical subunits.The pI of SGL is about 5 8 by isoelectric...

The possible role of lectin on the recognition between legume and rhizobium was discussed.The Stylosanthes gracilis lectin(SGL) has been extracted with ammonium sulfate and purified by chromatography on DEAE 32 column and gel filtration on Sephadex G 150 SGL exhibits one single band of molecular weight about 24 kD on SDS PAGE,while SGL's molecular weight is 45 kD estimated by gel filtration.It indicates that the lectin molecular contains two identical subunits.The pI of SGL is about 5 8 by isoelectric focusing.SGL is a glycoprotein and has sugar content of 2 6% SGL can agglutinate with the rabbit erythrocytes strongly,but it cannot agglutinate with human erythrocytes(A,B,AB or O type).D manose is just the sugar which can combine with SGL specifically and inhibit agglutination.SGL is an strong mitogen.The transformation ratio of human peripheral blood lymphocytes is 71 7% and the cell division ratio is 12 1%.SGL is labeled with fluorescein isothiocyanate(FITC) by the method of dialyze,and the product FITC SGL is used to treat 15 rhizobium strains.The result shows that FITC SGL can combine specifically with 7 of the 9 strains that nodulated Stylosanthes gracilis and only 1 of the 6 strains that unnodulated Stylosanthes gracilis .It means that there is a positive biological correlation between lectin binding capacity and nodulating ability.

为了探讨凝集素在豆科植物与根瘤菌的识别过程中的作用,用DEAE-32离子交换层析和SephadexG-150凝胶过滤分离、纯化格拉姆柱花草种子凝集素(SGL),其分子量约为45kD,由两个相同的亚基组成,等电点约pH5.8,它是一种糖蛋白,含糖量约为2.6%.SGL的热稳定性强.SGL的血凝活性能被甘露糖所抑制.SGL对红细胞的凝集作用可能具有种属专一性;SGL具有强的促有丝分裂作用;荧光标记实验显示:9株能与格拉姆柱花草植株结瘤的菌株有7株能与SGL结合,6株不能与之结瘤的菌株,只有1株能与SGL结合,这表明不同根瘤菌菌株对SGL的结合能力,和它们在格拉姆柱花草上结瘤能力之间可能具有一定的生物学相关性.

Objective To study the possible relationship between serum underglycosylated IgA1 and clinical feature,renal pathology and short-term curative effect.Methods Serum level of IgA1 was determined by ELISA in 68 cases with IgA nephropathy and 20 cases of control cases who suffered from nephritic minimal disease.Results The vicia villosa lectin binding capacity to serum IgA1 in IgA nephropathy was significantly higher than that in control (4.56 AU/mg±3.08 AU/mg vs 2.83 AU/mg± 1.02 AU/mg,P<0.025).The age,serum...

Objective To study the possible relationship between serum underglycosylated IgA1 and clinical feature,renal pathology and short-term curative effect.Methods Serum level of IgA1 was determined by ELISA in 68 cases with IgA nephropathy and 20 cases of control cases who suffered from nephritic minimal disease.Results The vicia villosa lectin binding capacity to serum IgA1 in IgA nephropathy was significantly higher than that in control (4.56 AU/mg±3.08 AU/mg vs 2.83 AU/mg± 1.02 AU/mg,P<0.025).The age,serum IgA concentration and Ccr were significantly lower(P<0.05)in serum IgA1 underglycosylation group than those normal glycosylation group in IgA nephropathy,but the level of serum IgA1 glycosylation was shown no correlation with the duration of disease,the levels of proteinuria,blood pressure,renal pathology and short-term curative effect.Conclusion Serum IgA1 glycosylation was abnormal in IgA nephropathy.The patients of serum serious underglycosylated IgA1 was younger and lower renal function.The degree of renal pathology and short-term effective could be speculated by the level of underglycosylated IgA1.

目的 探讨IgA肾病患者血清低糖基化IgA1的程度与临床表现、肾脏病理及近期疗效间的关系。方法 用ELISA法对 68例IgA肾病及 2 0例肾病综合征肾小球微小病变 (MCD )患者血清IgA1糖基化程度进行检测 ,并经治疗后随访。 结果 IgA肾病患者蚕豆凝集素与血清IgA1的结合力明显高于对照组 (P <0 .0 2 5 )。IgA肾病患者中 2 0例低糖基化组和 48例正常糖基化组比较 ,前者发病年龄轻、血IgA浓度及肌酐清除率低于后者 (P值均 <0 .0 5 ) ,而病程、尿蛋白排泄量、总胆固醇水平、血压、肾小球病理分级和近期疗效比较差异无显著性 (P值均 >0 .0 5 )。结论 IgA肾病患者血清IgA1呈低糖基化 ,血清低糖基化程度重者发病年龄较轻 ,但肾功能损伤较重 ,血IgA1低糖基化程度不能推测肾脏病理轻重或近期疗效

 
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