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upstream regulation region
相关语句
  上游调控序列
    Identification of 5-flank upstream regulation region of CD226
    CD226基因5′上游调控序列研究
短句来源
    Objective:To identify the function of the 5′-flank upstream regulation region of human CD226 gene.
    目的:研究CD226基因5′上游调控序列对CD226基因表达调控的影响。
短句来源
    Methods:The upstream regulation region of CD226 was cloned by PCR and ligated into pGL3 vector.
    方法:通过基因克隆的方法将CD226基因5′上游调控序列,克隆到荧光素酶报告基因载体中(pGL3basic),用脂质体转染Jurkat细胞,48小时后检测荧光素酶活性。
短句来源
    Aim To identify the promoter sequence and 5' terminal upstream regulation region of CD226. Methods We predict promoter sequence and 5'-terminal regulation region by software, then analysis one single nucleic acid polymorphism site in open reading frame of CD226 by sequencing.
    目的 研究人CD2 2 6 (PTA1)启动子及其 5’上游调控序列。 方法 应用计算机软件预测启动子序列 ,并分析人CD2 2 6基因 5’上游调控序列以及通过RT -PCR的方法研究开放读框中的单核苷酸多态性。
短句来源
  上游调控序列
    Identification of 5-flank upstream regulation region of CD226
    CD226基因5′上游调控序列研究
短句来源
    Objective:To identify the function of the 5′-flank upstream regulation region of human CD226 gene.
    目的:研究CD226基因5′上游调控序列对CD226基因表达调控的影响。
短句来源
    Methods:The upstream regulation region of CD226 was cloned by PCR and ligated into pGL3 vector.
    方法:通过基因克隆的方法将CD226基因5′上游调控序列,克隆到荧光素酶报告基因载体中(pGL3basic),用脂质体转染Jurkat细胞,48小时后检测荧光素酶活性。
短句来源
    Aim To identify the promoter sequence and 5' terminal upstream regulation region of CD226. Methods We predict promoter sequence and 5'-terminal regulation region by software, then analysis one single nucleic acid polymorphism site in open reading frame of CD226 by sequencing.
    目的 研究人CD2 2 6 (PTA1)启动子及其 5’上游调控序列。 方法 应用计算机软件预测启动子序列 ,并分析人CD2 2 6基因 5’上游调控序列以及通过RT -PCR的方法研究开放读框中的单核苷酸多态性。
短句来源
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Aim To identify the promoter sequence and 5' terminal upstream regulation region of CD226. Methods We predict promoter sequence and 5'-terminal regulation region by software, then analysis one single nucleic acid polymorphism site in open reading frame of CD226 by sequencing. Results We get two possible promoter sequence of CD226 and some putative nuclear factor binding sites through which nuclear factors may play some role in it, such as AP-1, Ets-1, Sp1, P300, PU.1 and PEA3. There is...

Aim To identify the promoter sequence and 5' terminal upstream regulation region of CD226. Methods We predict promoter sequence and 5'-terminal regulation region by software, then analysis one single nucleic acid polymorphism site in open reading frame of CD226 by sequencing. Results We get two possible promoter sequence of CD226 and some putative nuclear factor binding sites through which nuclear factors may play some role in it, such as AP-1, Ets-1, Sp1, P300, PU.1 and PEA3. There is one SNP site in the cytoplasmic part of CD226. Conclusion The expression of CD226 may be subtly regulated by many transcription factors and 5' upstream regulator sequence.

目的 研究人CD2 2 6 (PTA1)启动子及其 5’上游调控序列。方法 应用计算机软件预测启动子序列 ,并分析人CD2 2 6基因 5’上游调控序列以及通过RT -PCR的方法研究开放读框中的单核苷酸多态性。结果 CD2 2 6基因在 -375bp处和 - 130bp处可能有两个启动子 ,含有AP - 1、Ets- 1、Sp1、P30 0、PU .1、PEA3等转录因子结合序列 ,在编码胞浆区的序列中存在一个单核苷酸多态性位点。结论 CD2 2 6基因表达受到多种转录因子和 5’端上游调控序列的调控。

Objective:To identify the function of the 5′-flank upstream regulation region of human CD226 gene.Methods:The upstream regulation region of CD226 was cloned by PCR and ligated into pGL3 vector. Then the vector was transfected into Jurkat cell and luciferase activity was detected after 48 h culture.Results:CD226 gene may have two promoters, P1 and P2,which were located at the region of -843--319 bp and +1-+181 bp respectively, and PMA can up-regulate P1 while down-regulate P2. Both P1 and P2...

Objective:To identify the function of the 5′-flank upstream regulation region of human CD226 gene.Methods:The upstream regulation region of CD226 was cloned by PCR and ligated into pGL3 vector. Then the vector was transfected into Jurkat cell and luciferase activity was detected after 48 h culture.Results:CD226 gene may have two promoters, P1 and P2,which were located at the region of -843--319 bp and +1-+181 bp respectively, and PMA can up-regulate P1 while down-regulate P2. Both P1 and P2 can be up-regulated by A23187, especially P2.Conclusion:CD226 gene may have two promoters, and PMA and A23187 can regulate CD226 promoter activity in the similar pattern of protein level.

目的:研究CD226基因5′上游调控序列对CD226基因表达调控的影响。方法:通过基因克隆的方法将CD226基因5′上游调控序列,克隆到荧光素酶报告基因载体中(pGL3basic),用脂质体转染Jurkat细胞,48小时后检测荧光素酶活性。结果:CD226基因存在两个启动子P1和P2,分别位于与-843~-319bp和+1~+181bp,PMA可以上调P1的启动子活性,对P2有一定的抑制作用;A23187均可以上调两个启动子的活性,但对P2的作用更为明显。结论:CD226基因存在两个启动子,其活性受到PMA和A23187的调控,并呈与蛋白水平表达调控相类似的模式。

 
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