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This paper reviewed the microbial origins which were responsible for anaerobic biodegradation of chlorinated aromatic compounds. Microbial structures and functions in mixed culture were discussed The applicable fields of reductive dechlorinating microorganisms were also presented 本文综述了氯化芳香族化合物厌氧生物降解的微生物源,微生物结构与功能;讨论了具有脱氯活性厌氧微生物的可应用领域 Jointly toxic effects of acetochlor and Cu~(2+) on indigenous bacterial communities in phaeozem were studied using the traditional toxicity assessment methods combined with the modern molecular techniques. It was indicated that acetochlor and Cu~(2+) had a synergic inhibitory effect on the amount of culture-dependent viable bacteria including nitrogen-fixing bacteria, silicate bacteria and phosphorus-mineralization bacteria, as well as the activity of soil dehydrogenase. The increase in the substrate-induced... Jointly toxic effects of acetochlor and Cu~(2+) on indigenous bacterial communities in phaeozem were studied using the traditional toxicity assessment methods combined with the modern molecular techniques. It was indicated that acetochlor and Cu~(2+) had a synergic inhibitory effect on the amount of culture-dependent viable bacteria including nitrogen-fixing bacteria, silicate bacteria and phosphorus-mineralization bacteria, as well as the activity of soil dehydrogenase. The increase in the substrate-induced respiration (SIR) was significantly promoted by the two pollutants. Analyzing the similarity and the banding patterns by the denaturing gradient gel electrophoresis (DGGE) revealed that the joint toxicity of acetochlor and Cu~(2+) can significantly affect bacterial community structure. Microbial structures of the polluted soils and the clean soil without pesticide application were different, but there was a similarity in microbial structures between the soil with long-term pesticide application and the soil treated simultaneously with acetochlor and Cu~(2+) under the experimental condition, of which the similarity coefficient was up to 74.1%. 采用传统毒性评价手段和现代分子生物学技术相结合的方法 ,研究乙草胺和金属铜的复合污染对黑土农田生态系统中土著细菌群落的影响 .结果表明 ,乙草胺 铜复合污染比其单一污染更能降低细菌总数以及固氮菌、硅酸盐细菌和矿化磷细菌活菌量和土壤脱氢酶的活性 ,更能促进土壤呼吸强度的增加 ;利用以 16SrDNA为基础的变性梯度凝胶电泳 (DGGE)分析各处理样品间的相似程度 ,发现复合污染明显影响微生物群落结构 .由相似系数结果表明 :长期未施农药土壤、农药单一与复合污染土壤 3者间存在细菌群落的显著差异 ,并且乙草胺 Cu2 + 复合污染土壤与长期施用农药土壤具有较高相似性 (相似系数达 74 1% ) . It is reported that without cultivation, DNA could be directly extracted from environmental samples with molecular biological methods, such as polymerase chain reaction(PCR) and denaturting gradient gel electrophoresis(DGGE). To analyze the community diversity of activated sludge and bio-film in the municipal sewage, work was done to directly extrude crude DNA from activated sludge and bio-film samples, separate and amplify 16S rDNA by PCR and sequence it with DGGE. The results show the significant microbe community... It is reported that without cultivation, DNA could be directly extracted from environmental samples with molecular biological methods, such as polymerase chain reaction(PCR) and denaturting gradient gel electrophoresis(DGGE). To analyze the community diversity of activated sludge and bio-film in the municipal sewage, work was done to directly extrude crude DNA from activated sludge and bio-film samples, separate and amplify 16S rDNA by PCR and sequence it with DGGE. The results show the significant microbe community difference between cultivated and uncultivated activated sludge. Further research on the community diversity of two different sewage treatment processes was done and initial discussion on the microbial distribution in the same reactor and microbial structure in different experimental conditions was carried out. The sequences of several 16S rDNA DGGE fragments were determined and some possible bacteria were confirmed in comparision in GeneBank(NCBI). The results show that the PCR-DGGE technology combined with sequences determination is a feasible and efficient method for microorganism analysis in environmental sample. 通过PCR DGGE等分子生物学技术可以不经过常规培养直接从活性污泥和生物膜样品中提取DNA ,对 16SrDNAV3区进行PCR扩增 ,结合DGGE(变性梯度凝胶电泳 ) ,从而分析活性污泥与生物膜中微生物种群结构 .研究证实 ,活性污泥培养前后微生物种群结构发生很大的改变 .同时对 2种污水处理工艺中微生物种群结构进行了对比研究 ,对同一反应器不同位置微生物分布以及不同工况下的微生物种群结构进行了初步探讨 .测定了活性污泥中部分菌种的 16SrDNAV3区片段序列 ,通过NCBI(美国国立生物技术信息中心 )基因库比对 ,初步确定细菌的属 .结果显示 ,PCR DGGE结合测序技术是一种完全可行的快速进行环境学样品微生物研究的分析方法
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