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plaque forming cells pfc
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     (2)Glycyrrhizin greatly inhibited the forming of plaque units.
     (2)甘草甜素能明显减少空斑的形成。
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     Cell culture and plaque forming test of five arboviruses
     5种虫媒病毒的细胞培养及空斑试验
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     Canine distemper virus strain colned with plaque forming test
     犬瘟热病毒的蚀斑克隆试验
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     forming works;
     形成作品;
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     Cutting and Forming
     切削加工与塑性加工 两大加工方法的技术革新与市场变化
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  plaque forming cells pfc
pertussis', cultured spleen cells from treated mice were measured for their ability to produce plaque forming cells (PFC), in vitro, and to participate in mixed cell interactions, in vitro.
      
Effects of a single intraperitoneal (i.p.) injection of cadmium (Cd) on the primary humoral antibody responses against sheep red blood cells (SRBC) in mice were studied by assaying splenic plaque forming cells (PFC).
      


A modified method of Jerne's assay was used to determine haemolytic plaque-forming cells (PFC) in mouse spleen. By means of this method we measured the dynamic changes of the immune status in tumor-bearing mice. The tumors used were solid Sarcoma 180 maintained in Tian-jin albino mice, four ascitic tumors including Sarcoma 180 maintained in Tian-jin albino and 615 strain mice, and Hepatoma and Ehrlich carcinoma maintained in C57BL mice. The results. showed that spleen PFC decreased...

A modified method of Jerne's assay was used to determine haemolytic plaque-forming cells (PFC) in mouse spleen. By means of this method we measured the dynamic changes of the immune status in tumor-bearing mice. The tumors used were solid Sarcoma 180 maintained in Tian-jin albino mice, four ascitic tumors including Sarcoma 180 maintained in Tian-jin albino and 615 strain mice, and Hepatoma and Ehrlich carcinoma maintained in C57BL mice. The results. showed that spleen PFC decreased as the tumors progressed, and was hardly detected when the tumors were far advanced. The tumor-bearing mice commonly had striking splenomegaly with. decrease in number of small lymphocytes and. increase of 'immature' plasma cells, which might explain the poor immune responses of tumor-bearing mice.

本文应用改良的Jerne氏方法测定小鼠脾脏溶血空斑形成细胞。用此方法测定一株实体瘤(在津白小鼠上移植之肉瘤180)和四株腹水瘤(津白小鼠上移植之肉瘤180,615小鼠上移植之肉瘤180,以及C57BL小鼠上移植之肝癌和艾氏癌)带瘤动物免疫状态的动态变化。结果表明,随着肿瘤进行性生长,小鼠脾脏溶血空斑形成细胞逐渐减少,到晚期几乎不能测得。带瘤小鼠的脾脏普遍增大,病理组织学观察提示带瘤小鼠溶血空斑反应之低下与其脾脏的病理学改变相关。

The response of EAC-bearing host to thymus-dependent antigen (TDA) was assayed by plaque-forming cell (PFC) and hemagglutination (HA) test. It was found that the number of antibody-forming cell and titer of antibody to SRBC decreased with EAC development. Transfer of normal thymocytes and spleen cells could enhance the response of recipent to TDA, but not with the EAC-bearing donor lymphocytes. Slpecn cells or thymocytes plus normal bone marrow cells from...

The response of EAC-bearing host to thymus-dependent antigen (TDA) was assayed by plaque-forming cell (PFC) and hemagglutination (HA) test. It was found that the number of antibody-forming cell and titer of antibody to SRBC decreased with EAC development. Transfer of normal thymocytes and spleen cells could enhance the response of recipent to TDA, but not with the EAC-bearing donor lymphocytes. Slpecn cells or thymocytes plus normal bone marrow cells from EAC-bearing mice, contrary to normal donor, were inefficient in immunological reconstitution when they transplanted into lethally γ-rays irradiated recipents. The results suggested that deficiency of host response to TDA might be associated with decline of helper activity in thymus and spleen of tumor-bearing mice.

本文采用溶血空斑(PFC)和血凝(HA)试验探讨 Ehrlich 腹水癌(EAC)带瘤鼠在不同程度胸腺萎缩时对胸腺依赖性抗原(TDA)的反应性。发现接种 EAC后,抗体形成细胞数量和血清抗体效价逐渐下降;用带瘤鼠的胸腺和脾细胞转移不能促进受鼠对TDA的反应性,其胸腺细胞加骨髓细胞或脾细胞移植对致死性γ射线照射小鼠的免疫重建能力,明显低于正常对照组。结果表明:带瘤鼠在胸腺萎缩后,对TDA的反应能力明显下降,其机制可能与宿主胸腺和脾中的辅助细胞活性下降有关。

APS,an extract of polysaccharide from Astragalus mongolicus,increased the spleen weight of normal mice at a dosage of 100mg/kg qd.for 6 days.This effect was significantly decreased in thymectomized mice injected with anti-thymus cell serum(T_x-ATS).APS potentiated significantly both the direct and indirect plaque-forming cells(PFC)in normal mice following SRBC immunization,but it did not potentiate direct PFC in T_x-ATS mice. APS could potentiate d-PFC response to LPS...

APS,an extract of polysaccharide from Astragalus mongolicus,increased the spleen weight of normal mice at a dosage of 100mg/kg qd.for 6 days.This effect was significantly decreased in thymectomized mice injected with anti-thymus cell serum(T_x-ATS).APS potentiated significantly both the direct and indirect plaque-forming cells(PFC)in normal mice following SRBC immunization,but it did not potentiate direct PFC in T_x-ATS mice. APS could potentiate d-PFC response to LPS immunization in T_x-ATS mice,but this effect was lower than that in normal mice given SRBC.These results suggested that the mechanism of immunostimulatory effect of APS might involve principally an induction of T lymphocyte activity in the body.

黄芪多糖(APS)100mg/kgx6天 ip 可以增加正常小鼠脾脏重量,而对去胸腺与抗胸腺细胞血清处理过的小鼠(T_x-ATS)的作用明显降低。APS 能够显著提高正常小鼠 SRBC 免疫后脾细胞直接溶血空斑(d-PFC)和间接溶血空斑(i-PFC)数量,但对 T_x-ATS 小鼠不能促进d-PFC 数升高。APS 可以提高 T_x-ATS 小鼠在 LPS 免疫后 d-PFC 数,这个作用同 APS 对正常小鼠 SRBC 免疫后的效应相比显著降低。这些结果提示,APS 促进抗体形成作用机理可能是通过 T 细胞介导的。

 
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