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mechanism of thermostability
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  “mechanism of thermostability”译为未确定词的双语例句
     Studies on the Mechanism of Thermostability and Thermophilicity Change of Thermostable Alkaline Phosphatase and Its Mutants
     耐热碱性磷酸酶突变体耐热性变化机理研究
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     The work provided the direction for mutation to improve enzymatic specific activity and studying the mechanism of thermostability and thermophilicity.
     为提高该酶的比活力和研究其热稳定性及亲热性提供了突变方向。
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  相似匹配句对
     Its mechanism is discussed.
     探讨了PMAM的阻垢缓蚀机理。
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     The mechanism has been designed.
     2.完成了系统机械结构的设计。
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     The Mechanism of Thinking
     思维机制探索
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     The Reliability of Mechanism
     机构可靠性
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     Thermostability of racemic neoclausenamidone
     外消旋新黄皮酰胺酮的热稳定性研究
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:Through PCR-mediated mutagenesis, three mutants E68S, S70A and E68SS70A around active site S(69) were obtained. Their enzymatic characteristics was determined. It was found that the specific activity of E68S ascended 8 times while its optional reactive temperature climbed 20℃ and its Tm descended 3℃; the specific activity of S70A ascended 1 time while its optional reactive temperature climbed 5℃ and its Tm descended 2℃; the specific activity of E68SS70A descended 50% while its optional reactive temperature...

:Through PCR-mediated mutagenesis, three mutants E68S, S70A and E68SS70A around active site S(69) were obtained. Their enzymatic characteristics was determined. It was found that the specific activity of E68S ascended 8 times while its optional reactive temperature climbed 20℃ and its Tm descended 3℃; the specific activity of S70A ascended 1 time while its optional reactive temperature climbed 5℃ and its Tm descended 2℃; the specific activity of E68SS70A descended 50% while its optional reactive temperature climbed 5℃ and its Tm descended 19℃. These result implied that the amino acids, beside the active site, were contributed not only to enzymatic activity but also to its thermostability and thermophilicity. The work provided the direction for mutation to improve enzymatic specific activity and studying the mechanism of thermostability and thermophilicity.

通过对耐热碱性磷酸酯酶TAPND27活性位点S(69)两侧的E(68)和S(70)的定点突变,得到了3个突变子E68S、S70A和E68SS70A。在蛋白纯化的基础上测定了3个变体的一些酶学性质,与TAPND27相比,E68S的比活力上升了8倍,Tm下降了3℃,最适反应温度上升了20℃;S70A的比活力上升了1部,Tm下降了2℃,最适反应温度上升了5℃;E68SS70A的比活力下降了50%,Tm下降了19℃,最适反应温度上升了5℃。说明活性位点S(69)两侧的氨基酸残基不仅和酶蛋白的催化能力,而且与酶的热稳定性和亲热性有很大的关系。为提高该酶的比活力和研究其热稳定性及亲热性提供了突变方向。

The relationship among the substituted amino acids, the 3D structure simulated on PC through CPHmodels Server (http://www.cbs.dtu.dk/services/CPHmodels/) and the thermostable performance of 4 thermostable alkaline phosphatase(TAP) mutants selected from a clone bank of more than 200 mutants were analyzed to explore the mechanism of thermostability change. These mutants are TAP A410T(A410→T),TAP P396S(P396→S),TAP 2(N100→S、T320→I) and TAP 4(N100→S、P396→S、A410→V、P490→S). TAP and the mutants' thermostable...

The relationship among the substituted amino acids, the 3D structure simulated on PC through CPHmodels Server (http://www.cbs.dtu.dk/services/CPHmodels/) and the thermostable performance of 4 thermostable alkaline phosphatase(TAP) mutants selected from a clone bank of more than 200 mutants were analyzed to explore the mechanism of thermostability change. These mutants are TAP A410T(A410→T),TAP P396S(P396→S),TAP 2(N100→S、T320→I) and TAP 4(N100→S、P396→S、A410→V、P490→S). TAP and the mutants' thermostable performance was evaluated by measuring the highest tolerable temperature (T 1/2) and the optimal reaction temperature (T opt). The 3D structure neighboring the substituted amino acids was simulated by Swiss-PDBViewer to observe the relationship between the structure change and the thermostable performance of TAP and its mu- tants. The results displayed that all these amino acid substitutions except the T320→I mutant brought about only a little local change on TAP's 3D structure and very little effect on their optimal reaction temperature, but a significant decrease(nearly 10℃) on their highest tolerable temperature. However, the T320→I mutation due to close to TAP's active sites did bring about a significant descendents of the mutant in both the highest tolerable temperature and the optimal reaction temperature. Thus, it seems to be able to conclude that most of the amino acid substitutions, no matter where they locate and what structure change they may make, can cause TAP's highest tolerable temperature reduced significantly. What's more, if the mutation occurring near or in the active sites, it can also cause TAP's optimal reaction temperature reduced significantly at the same time.

从耐热碱性磷酸酶 (TAP) 2 0 0多个随机突变体的克隆库中选出耐热性明显下降的 4株突变体 ,进行全序列及表达产物的最高耐受温度和最适反应温度测定和酶分子高级结构的模拟 ,分析突变位点、高级结构和耐热性表现三者的关系 ,探讨引起耐热性变化的机理。结构模拟显示所有突变位点都仅能引起细微的、局部的结构变化 ,除T3 2 0→I外都未直接触及酶的活性中心 ;结构上的细微改变虽然对最适反应温度影响不明显 ,但却使最高耐受温度降低了 10℃左右 ;T3 2 0→I靠近酶的活性中心 ,尽管未能引起结构的较大变化 ,但却使最高耐受温度和最适反应温度同时显著降低。可见 ,多数点突变对高级结构的影响都不剧烈 ,但对耐热性尤其是最高耐受温度的影响却比较明显 ,一般地 ,在非活性区的突变通常只能引起最高耐受温度的降低 ,靠近活性区的突变则能同时引起最适反应温度和最高耐受温度的降低

Thermophiles and hyperthermophiles screened from extreme environments are the main source of thermozymes. Because of thermophiles' harsh cultural condition and low enzyme quantities, gene engineering and protein engineering are also methods of obtaining thermozymes. At the present time, It is a major method of research on the thermostability of thermozymes to compare the homology of amino acid sequence between thermozymes and mesophilic enzymes. The different enzymes have different mechanisms of thermostability....

Thermophiles and hyperthermophiles screened from extreme environments are the main source of thermozymes. Because of thermophiles' harsh cultural condition and low enzyme quantities, gene engineering and protein engineering are also methods of obtaining thermozymes. At the present time, It is a major method of research on the thermostability of thermozymes to compare the homology of amino acid sequence between thermozymes and mesophilic enzymes. The different enzymes have different mechanisms of thermostability. The amino acid composition between thermozymes and their mesophilic counterparts is different in the primary structure. α-helix stabilization increases thermozymes' stability in secondary structure. In advanced structure, internal factors of thermophilic enzymes are of importance, such as "additional" ionic networks, hydrophobic interactions, hydrogen bonds, cooperative association, etc. Some environmental factors have effect on thermostability of thermozymes.

从极端环境中筛选的嗜热菌、超嗜热菌(包括古菌)是嗜热酶的主要来源,但由于嗜热菌的培养条件严格、产酶量低等缺点,使基因工程及蛋白质工程成为生产嗜热酶的又一手段。目前,比较嗜热酶与同源常温酶的氨基酸序列的同源性是研究嗜热酶耐热性的主要方法。不同的酶其耐热机制不同,在一级结构中,嗜热酶具有与同源常温酶不同的氨基酸组成。α-螺旋的稳定性使嗜热酶在二级结构上更稳定。在高级结构中,“额外”的离子键、疏水作用、氢键、寡聚体的形成等对嗜热酶的耐热性起主要作用。一些环境因子也影响嗜热酶的耐热性。

 
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