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This experiment studies on in vitro selection of mutant clones resistant to herbicide by way of callus cells and its regenerated plantlets of Guitang 11 exposed to gamma rays of Co60 and then to culture medium with herbicide. The results show that the sun'ival rate of callus cells and its regenerated plantlets cultured on medium full mixed with herbicide is higher than that of those co-cultured with herbicide on solid medium. 本试验采用桂糖11号细胞团和幼苗作离体培养,用Co60-γ射线35Gy剂量照射后,分别转入含除草剂的培养基中,用除草剂作为选择压力,研究离体培养条件下抗除草剂突变体的筛选技术和方法。实验结果表明,经诱变培养法处理的细胞团及幼苗的存活率比经共培养法处理的高。
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