Comparison of Ethanol Tolerance in a Self-flocculating Fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae Employing Mg~(2+) or Ca~(2+) as Supplement
Mg~(2+) (as MgSO_4) at 3.5 mmol/L remarkably increased ethanol tolerance of fusant SPSC. After 9 h of exposure to 20% (volume fraction) ethanol at 30 ℃, no viability remained for the control whereas 53.1% remained for the cells both grown and incubated in Mg~(2+)-added medium.
This paper studied the yeast in rotten grape and barmy honey of Elsholtzia rugulosa by natural selection,UV mutation and ethanol tolerance fermentation, the mutant A15 yielded alcohol at a high level of 10.4%(V/V),and showed strong genetic stability during the generation.
Thus, that Mg~(2+) enhances ethanol tolerance of this strain is closely related to its capability to reduce plasma membrane permeability of yeast cells under ethanol stress.
The low ethanol tolerance of thermophilic anaerobic bacteria, generally less than 2% (v/v) ethanol, is one of the main limiting factors for their potential use for second generation fuel ethanol production.
Ethanol-tolerant and thermo-tolerant yeast strain Saccharomyces cerevisiae C11-3 cells immobilized in calcium pectate and calcium alginate gels were used for ethanol fermentation in a three-reactor system with a gradient temperature control.
These results suggest that the activation of PKC potentiates NMDA receptor function of the rat line which is more sensitive to alcohol (HAS) but does not affect [3H]MK-801 binding of alcohol resistant (LAS) rats.
The results confirm the existence of acute tolerance to ethanol, as defined by reduced impairment of function for a given blood level on the falling versus the rising arm of the blood alcohol curve.
Ammonium molybdate has been reported to have a protective effect on tetramethylbenzidine (TMB) reaction product against alcohol. It was intended in this study to differentiate its effect against alcohol and high pH. Horseradish peroxidase (HRP)labeled sections were treated with ammonium molybdate after incubation with TMB. Subsequent soaking in alcohol buffered to pH 3.3 for as long as 30 minuts was proved to have little fading effect on TMB product. Sections in phosphorate buffer solution ( pH 7.4 ).howeve...
This paper described the general characteristics of S.cerevisiae 1450 and S. bayanus A_(m-1) including their morphological features, growth and physiological characteristics. The effect of ethanol concentration on cell viabili ty,growth rate as well as fermentation functions was also tested.The SO2 added to must effected the ADWY yeasts performance during the main fermentation. The temperature of the fermentation had obvious influence on the composition and flavour of wine made by both ADWY. The analytical ...
In this paper, the test tube experiments of the HRP-TMB reaction and the com parison of the various TMB histochemical procedures in the caudate-putamen-substantia nigra projection model of the rat were performed in order to improve the quality of the HRP-TMB method. As a result, a new HRP-TMB procedure using sodium tungsta te (ST) as a stabilizer (TMB-ST method) is developed for the tract-tracing tech nique with HRP, The sensitivity of this new method is markedly superior to that of Mesulam's TMB method. Fu...