Protein Amino Acid Composition of Plasma Membranes Affects Membrane Fluidity and Thereby Ethanol Tolerance in a Self-flocculating Fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae
Ca 2+ at 1 64 mmol/L markedly increased ethanol tolerance of a self_flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae. After 9 h of exposure to 20%(V/V) ethanol at 30℃ , no viability remained for the control whereas 50 0% remained for the cells both grown and incubated with ethanol in Ca 2+_added medium.
Therefore, the enhancing effect of Ca 2+ on ethanol tolerance of this strain is closely related to its ability to decrease plasma membrane permeability of yeast cells subjected to ethanol stress.
A novel relationship between phospholipid fatty acid composition of plasma membrane and yeast ethanol tolerance, and its mechanism are revealed in this work.
In the malt juice,adding 0.02%K+ or 0.03% A13+ can increase the production strikingly and adding some degree concentration trahalose can increase the production too and cut short the time of taming.
These strains had some specific morphological features, and their optimal growth temperature was 30~35 ℃. The optimal pH of spore budding and mycelium growth was 4.0~5.5, the resistance ability to ethanol was as high as 15%(V/V).
The results showed that the No. 31 strain is best among such production performanc as acid-producing velocity,alcohol degree resistance,bottom-acid resistance,and can resist large temperature range,so it is the uperior acetobacter acetic.
The low ethanol tolerance of thermophilic anaerobic bacteria, generally less than 2% (v/v) ethanol, is one of the main limiting factors for their potential use for second generation fuel ethanol production.
The results confirm the existence of acute tolerance to ethanol, as defined by reduced impairment of function for a given blood level on the falling versus the rising arm of the blood alcohol curve.
Saccharomyces sabe Yabe is a Japanese sake--producins strain wnich is ash ethanol--tolerant; Saccharomyces cereLd3tae K is a high ethand-producing strain that formentation ability is strong. Protoplast fusion between SaccharomgCeS sake Yabe and SacChaTomgCes cererihec K has been stUdied. We obtained the auxotrophic mutantS of k (lys--,p-- ) and Q(arg- ) by mutation with DES. The frequency of fusion is 1. 25 per 105 protoplast. Comparative stidies of cell size, cell morphology and formetation ability etc. be...
Ethanol tolerance of high ethanol tolerant yeast Saccharomyces cerevisia 1200 and ethanol sensitive yeast Saccharomyces cerevisia K5 5A with and without glucose was analyzed At the same time, the content of trehalose during the shock of ethanol was analyzed The results showed that the ethanol tolerance of the yeast is dependent of the content of trehalose Trehalose can even protect the integrality of mitochondria membrane and prevent the loss of mitochondria DNA
The biological features of several Monascus strains which can produce blood\|lipids lowering materials were studied. These strains had some specific morphological features, and their optimal growth temperature was 30~35 ℃. The optimal pH of spore budding and mycelium growth was 4.0~5.5, the resistance ability to ethanol was as high as 15%(V/V).