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|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
High ethanol tolerance of the thermophilic anaerobic ethanol producer Thermoanaerobacter BG1L1
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The low ethanol tolerance of thermophilic anaerobic bacteria, generally less than 2% (v/v) ethanol, is one of the main limiting factors for their potential use for second generation fuel ethanol production.
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Ethanol tolerance was evaluated based on inhibition of fermentative performance e.g.
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The strain demonstrated a remarkable ethanol tolerance, which is the second highest displayed by thermophilic anaerobic bacteria known to the authors.
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This appears to be the first study of the ethanol tolerance of these microorganisms in a continuous immobilized reactor system.
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| | The protoplast fusions of Saccharomyces cereviae H-1 which resist high osmotic pressure and high alcohol concentration and K -1 which is alcohol production strains in the current industry obtained Saccharo myces cerevision HK - 6 which had not only the faster far mentaion speed dut also had the higher alcohol concentration. | | 选择耐高渗透压、耐高酒精度和发酵终了产酒精量较高的黄酒酵母H—1和目前酒精工业生产用菌K号酒精酵母K—1,运用细胞融合技术选育发酵速度快、产酒精量高的工业用酒精酵母.双倍体黄酒酵母H—1和双倍酒精酵母K—1经过产孢前预培养和产孢培养后,蜗牛酶水解子囊孢子壁,离心收集单倍体子囊孢子,培养后得到单倍体黄酒酵母H—2和单倍体酒精酵母K—2.用硫酸二乙醇诱变处理单倍体细胞,得到单倍体黄酒酵母的维生素缺陷型H—3和单倍体酒精酵母的氨塞骏营养缺陷型K—3通过正交试验找出了H—3和K—3原生质体形成及再生的较优条件是:对数生长后期的细胞33℃、0.2%的β—巯基乙醇预处理15分钟,然后4%蜗牛酶作用2小时.用35%聚乙二醇和10mMCaC1_2诱导融合40分钟,于再生基本夹层培养基上培养获得营养互补融合子,并且考查了融合子的遗传稳定性.通过耐酒精度、一发酵速度和最终产酒精量的测定,筛选出融合子HK—6.HK—6与生产用K号酒精酵母相比,发酵速度相接近,而最终产酒精量提高... | | 文摘来源 | | The supplement of 3.5mmol稬-1 Mg2+ or 1.64 mmol稬-1 Ca2+ increased the tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae to ethanol while Mg2+ exerted a stronger enhancing effect than Ca2+. After 9 h of exposure to 20% (v/v) ethanol at 30℃, all cells died whereas 53.1% and 50.0% remained viable for the cells both grown and incubated with ethanol in Mg2+ or Ca2+-added medium respectively. To study the way that each ion increased alcohol tolerance of this strain, ... | | 生长和冲击阶段均添加3.5mmolL-1 Mg2+ 或1.64 mmolL-1 Ca2+ 都能显著提高融合株SPSC于30℃在20% (v/v) 酒精冲击下的存活率。经过9 h冲击,对照组的存活率为0, 而添加Mg2+ 或Ca2+ 的存活率分别为53.1% 和50.0%,表明适当浓度的Mg2+ 或Ca2+ 均能显著提高菌体的耐酒精能力,但 Mg2+ 的促进作用强于Ca2+。通过考察 Mg2+ 和Ca2+ 对菌体于30℃在15% (v/v) 酒精冲击下细胞膜透性的影响发现,生长和冲击阶段均添加3.5 mmol稬-1 Mg2+ 或1.64 mmol稬-1 Ca2+ 的细胞膜透性系数(P?分别仅为对照组水平的15.6% 和 29.3%, 表明适当浓度的 Mg2+ 或 Ca2+ 均能显著降低受冲击菌体的细胞膜透性,但Mg2+ 降低膜透性能力强于Ca2+;而且,实验显示,添加Mg2+ 或Ca2+ 提高存活率与添加Mg2+ 或Ca2+ 降低P?存在直接的对应关系。因此,Mg2+ 和Ca2+ 提高融合株SPSC耐酒精能力均与各自降低受冲击菌体的细胞膜透性密切相关。 | | 文摘来源 | |   |
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