助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   bt-toxin protein 的翻译结果: 查询用时:0.007秒
图标索引 在分类学科中查询
所有学科
农业基础科学
更多类别查询

图标索引 历史查询
 

bt-toxin protein
相关语句
  bt毒蛋白
     Studies on maize transformation have been carried out through bombarding by particle gun, ultrasonicating in a DNA buffer or ovary injecting by a self-made mi-croinjector. The plasmid pB48.415, which carries a 3'-end truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation.
     用基因枪、超声波和子房注射法转化玉米,所用质粒pB48.415带有3'端截短的Bt毒蛋白基因和潮霉素磷酸转移酶(hpt)基因。
短句来源
  “bt-toxin protein”译为未确定词的双语例句
     Transfer of Bt-toxin Protein Gene into Maize and the Resistance of Transgenic Plants to Corn Borer
     玉米胚性愈伤组织转化及转Bt基因植株的抗虫性
短句来源
  相似匹配句对
     Comparison among Three ELISA Methods for Bt Toxin Protein
     3种ELISA法检测Bt杀虫蛋白的比较研究
短句来源
     Transgenic Corn with Bt Toxin Protein Gene and Insect-resistance
     转Bt毒蛋白基因玉米及其抗虫性研究进展
短句来源
     Detection of Bt Toxin Protein in Transgenic Cotton by Dot-ELISA
     利用Dot- ELIS A检测Bt棉杀虫蛋白的研究
短句来源
     Bt.
     Bt.
短句来源
     protein.
     protein。
短句来源
查询“bt-toxin protein”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
没有找到相关例句


In this study,an insecticidal crystal protein gene cryIA(c)of Bacillus thuringiensis via the broad host range plasmids pUCP18 and pUCP19 was transformed into biocontrol bacterium Pseudomonas fluorescens P303 by electroporation.southern blotting and Western blotting assays respectively verified the existence of cryIA(c)gene and expression of Bt toxin protein in the transformation.Results of bioassay showed that these new genetically engineered strains were not only inhibitory to the plant Pathogen...

In this study,an insecticidal crystal protein gene cryIA(c)of Bacillus thuringiensis via the broad host range plasmids pUCP18 and pUCP19 was transformed into biocontrol bacterium Pseudomonas fluorescens P303 by electroporation.southern blotting and Western blotting assays respectively verified the existence of cryIA(c)gene and expression of Bt toxin protein in the transformation.Results of bioassay showed that these new genetically engineered strains were not only inhibitory to the plant Pathogen Geumannomyces graminis var tritici, but also insecticidal to the corn borer and diamond back moth.

以广寄主范围质粒pUCP18和pUCP19为载体,用电激转化法,将苏云金杆菌杀虫蛋白基因crylA(c)片段导入了荧光假单胞菌P303菌株。Southern印迹分析和Western印迹分析分别证实crylA(c)基因的导入和晶体蛋白在P303中的表达。生物活性测定结果表明,新构建的荧光假单胞工程菌株不仅保持了野生型自然菌株对小麦全蚀病有良好的抑菌活性,而且表现出对小菜蛾、玉米螟有显著的毒杀作用。

Maize embryogenic calli were bombarded by high- velocity microprojectiles coated withplasmid pB48.4l5 DNA, which contained Bt-toxin protein gene and hygromycin phosphotransferase gene.After being selected on medium with hygromycin the resistant embryogenic calli were transferred to regen-eration medium on which embroids formed and germinated.The results of dot blotting and Southern hy-bridization certified the integration of Bt-gene into maize genome. The prelirninary results of insect resis-tance evaluation...

Maize embryogenic calli were bombarded by high- velocity microprojectiles coated withplasmid pB48.4l5 DNA, which contained Bt-toxin protein gene and hygromycin phosphotransferase gene.After being selected on medium with hygromycin the resistant embryogenic calli were transferred to regen-eration medium on which embroids formed and germinated.The results of dot blotting and Southern hy-bridization certified the integration of Bt-gene into maize genome. The prelirninary results of insect resis-tance evaluation on the progenies from one Bt-transgenic plant showed that the ratio of resistant and sus-ceptible plants was near to l:l.

用基因枪直接轰击玉米胚性愈伤组织,经潮霉素抗性选择平均每皿可得到0.7~2.0块抗性愈伤组织,且大部分可再生植株。对这些从抗性愈伤组织分化的植株进行点杂交和Southern吸印杂交的结果也证明Bt基因已插入玉米染色体组。对一个转Bt基因植株的测交后代进行了玉米螟抗性的田间鉴定,抗虫和感虫植株的比例接近1:1,符合孟德尔单显性基因的遗传规律。

Studies on maize transformation have been carried out through bombarding by particle gun, ultrasonicating in a DNA buffer or ovary injecting by a self-made mi-croinjector. The plasmid pB48.415, which carries a 3'-end truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation. Transgenic maize plants were obtained from immature embryos and embryogenic calli bombarded with particle gun, embryogenic calli ultrasonicated under different conditions or ovaries...

Studies on maize transformation have been carried out through bombarding by particle gun, ultrasonicating in a DNA buffer or ovary injecting by a self-made mi-croinjector. The plasmid pB48.415, which carries a 3'-end truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation. Transgenic maize plants were obtained from immature embryos and embryogenic calli bombarded with particle gun, embryogenic calli ultrasonicated under different conditions or ovaries injected after 10-20h of pollination. The results of Dot blotting and Southern blotting analysis certified the integration of Bt gene into maize genome.

用基因枪、超声波和子房注射法转化玉米,所用质粒pB48.415带有3'端截短的Bt毒蛋白基因和潮霉素磷酸转移酶(hpt)基因。用基因枪轰击玉米胚性愈伤组织和幼胚,超声波处理玉米胚性愈伤组织,用自制的微玻针注射授粉后10~20h的玉米子房,均已成功地获得了转Bt基因的玉米植株。点杂交和Southern吸印杂交的结果都证明在转基因玉米植株中存在Bt毒蛋白基因。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关bt-toxin protein的内容
在知识搜索中查有关bt-toxin protein的内容
在数字搜索中查有关bt-toxin protein的内容
在概念知识元中查有关bt-toxin protein的内容
在学术趋势中查有关bt-toxin protein的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社