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   mice sarcoma cell 的翻译结果: 查询用时:0.006秒
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mice sarcoma cell
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     Electrochemotherapy and Its Mechanism for Sarcoma of KM Mice
     电化学治疗昆明小鼠肉瘤及其机理分析
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     The mice sarcoma-180 was used as tumor strain.
     瘤株为小鼠肉瘤细胞(sarcoma-180)。
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     Mice were injected (i.m.)
     末次免疫后一周,眼眶取血后处死小鼠。
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     coli in mice;
     coli O_(138)生长的作用。
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     SARCOMA OF THE BREAST
     23例乳腺肉瘤临床病理分析
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A line of mouse sarcoma cell,designated as S_(180)-V,has been establis-hed by the suspension method.Theoriginal cultures were initiated on Jan,24,1978.After 6 passages,the growthrate appeared stabilized and the patternchanged from suspension to monolayercultures.S_(180) sarcoma have been describedas undifferentiated tumors.The S_(180)-V cells have large round or oval nuclei,irregular in size and shape.Thesetumor cells have no regular alignment.Occasionally cells are multinuc leat.An...

A line of mouse sarcoma cell,designated as S_(180)-V,has been establis-hed by the suspension method.Theoriginal cultures were initiated on Jan,24,1978.After 6 passages,the growthrate appeared stabilized and the patternchanged from suspension to monolayercultures.S_(180) sarcoma have been describedas undifferentiated tumors.The S_(180)-V cells have large round or oval nuclei,irregular in size and shape.Thesetumor cells have no regular alignment.Occasionally cells are multinuc leat.An inoculum of 5×10~4 cells/ml inRPMI 1640 culture medium at 37℃multiplies approximately 40 fold in 6days.The doubling time for S_(180)-Vcell population is 14—20 hours.Thegrowth saturated density for these cellsaverages 24×10~4/cm~2.The mitotic in-dex as determined by counting 3000—4000 cells daily showed approxima-tely 35‰.The length of the mitoticperiod in a cell cycle,as calculatedfrom mitotic index,is approximately1.2 hours.The S_(180)-V cells are com-posed of a rather heterogeneous cellpopulation with near tetraploid modes(54—108).The modal chromosomenumber of this cell is 88.Two or more types of markerchromosomes were found in theS_(180)-V cells.These include largemeta-dicentric,middle metacentric,small metacentric,submetacentric andminute chromosomes.Inocula prepared from these cul-tures produced typical sarcomas orascites in 100% of the mice injectedwith 10×15~5 cells.It is evident thatthese in vitro cultures of S_(180)-V havethus far retained the capacity to inducecharacteristic sarcoma in inbred or hy-brid mice.

S_(180)-V 系细胞,是通过悬浮静置培养过渡到单层静置培养而建成的。至今年6月已传至一百代。该系细胞形状不一,核大,常见单核或多核巨细胞。体外生长的细胞,约60%贴瓶生长,40%悬浮生长。悬浮生长的细胞,换瓶后仍能贴瓶生长。细胞群体倍增时间为14—20小时。分裂指数平均为35‰,分裂期的时长平均1.2小时。饱和密度约24×10~4/平方厘米。染色体众数值为88,具中双着丝点标记染色体。以10~6细胞/鼠的细胞数接种于小鼠,全部动物长成实体瘤或腹水瘤。

The proliferation and survival fraction of cultured mouse sarcoma cells treated with pingyangmycin (PYM) and calcium blockers: VP and Tmb8 cam inhibitors: wl3 and TFP alone or simultaneously were explored in this paper. It was shown that VP and wl3 within 1C10 decreased the IC50 for PYM by 7.4 and 10 folds respectively, but Tmb8 and TFP had very slight effects on the IC50 for PYM. Nontoxic—dose VP and wl3 enhanced the cytotoxicity of PYM apparently The results suggest that its hopeful to used VP and wl3...

The proliferation and survival fraction of cultured mouse sarcoma cells treated with pingyangmycin (PYM) and calcium blockers: VP and Tmb8 cam inhibitors: wl3 and TFP alone or simultaneously were explored in this paper. It was shown that VP and wl3 within 1C10 decreased the IC50 for PYM by 7.4 and 10 folds respectively, but Tmb8 and TFP had very slight effects on the IC50 for PYM. Nontoxic—dose VP and wl3 enhanced the cytotoxicity of PYM apparently The results suggest that its hopeful to used VP and wl3 as antitumor enhancers of PYM in clinical.

本文探讨了体外培养小鼠肉瘤细胞经平阳霉素和钙阻断剂:VP和Tmb8;钙调素抑制剂:W13和TFP单独或同时处理后细胞增殖和存活率的变化,发现在IC10以内;VP和W13能够分别降低平阳霉素IC10值7、4倍和10倍;而Tmb8和TFP对平阳霉素IC10无显著影响;无毒剂量的VP和W13能显著增强平阳霉素的细胞毒性。结果提示VP和W13有希望作为平阳霉素临床应用的抗癌增效药物

Immunosuppressive activities have been found in the culture supernatant of four leukemia cell lines and one mouse sarcoma cell line and in the sera of four patients with leukemia. The inhibitors suppressed PHA induced lymphocyte proliferation and IL-2 production and responsiveness of T cells to IL-2, but no inhibitory activity was observed in the culture supernatant of 2BS cell line. The culture supernatant of tumor cell lines was not cytoxic. The effects of tumor-derived immunosuppressive...

Immunosuppressive activities have been found in the culture supernatant of four leukemia cell lines and one mouse sarcoma cell line and in the sera of four patients with leukemia. The inhibitors suppressed PHA induced lymphocyte proliferation and IL-2 production and responsiveness of T cells to IL-2, but no inhibitory activity was observed in the culture supernatant of 2BS cell line. The culture supernatant of tumor cell lines was not cytoxic. The effects of tumor-derived immunosuppressive factor (TDSF) had no species and organ specifity. The molecular weight of TDSF from HL-60 human promyelocytic leukemia is about 87kD. It is protein in chemical nature. The synthesis and secretion of TDSF were partially inhibited by drugs of anti-acute non-lymphocytic leukemia.

4种人白血病细胞株,一种小鼠肉瘤细胞株的培养上清液和4种白血病患者血清均发现有免疫抑制活性。抑制PHA诱导的淋巴细胞增殖,抑制白细胞介素2的产生和反应性,以2BS人胚肺细胞作对照则无抑制作用.肿瘤细胞株培养上清液无细胞毒作用,其抑制作用无种属特异性和组织器官特异性.其中,来源于HL-60人早幼粒白血病细胞肿瘤免疫抑制因子的分子量为87kD,其化学本质为蛋白质,抗急性非淋巴细胞白血病药物对这种肿瘤免疫抑制因子的分泌有部分阻抑作用。

 
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