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expressive space
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     Broadening the Expressive Space of Easel Oil Painting
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  expressive space
Use of correspondence analysis reduced dimensions of sample-expressive space without losing initial information.
      


This paper presents the generation background of feature technology and the related concepts and methods of feature modelling. According to the requirements of integrated manufacturing system, a new kind of feature information description method and its system structure have been proposed, and the design feature library of bendings has been built up. So the product design based on features has been realized. The relations between feature expressive spaces and the principles and methods Of feature mapping...

This paper presents the generation background of feature technology and the related concepts and methods of feature modelling. According to the requirements of integrated manufacturing system, a new kind of feature information description method and its system structure have been proposed, and the design feature library of bendings has been built up. So the product design based on features has been realized. The relations between feature expressive spaces and the principles and methods Of feature mapping based on feature spaces have been analyzed and induced and the conversions from a design feature model of a bending to a manufacturing feature model have been realized. So the integration and share of product information have truly been achieved.

本文介绍特征技术产生的背景和特征建模的有关概念与方法。根据集成化制造系统的要求,提出了一套新的特征信息描述方法和系统结构;建立了折弯零件的设计特征库,实现了析弯零件的基于特征设计.分析、归纳了特征表达空间之间的关系和基于特征空间的特征映射原理与方法,完成了折弯零件设计特征模型到制造特征模型的转换,从而实现了产品信息的集成与共享.

In order to obtain an excellent Streptomyces host, regarding two glucose isomerase(GI) deficient strains with thiostrepton resistance named M1033XW78 and M1033XW194 as starting strains, one strain named M53 which was sensitive to thiostrepton. The glucose isomerase deficient strains was chosen by liquid culture, photocopy and negative filtration. The analysis of PCR amplification, DNA sequencing and restriction enzyme digestion demonstrated that the chromosome of M53 had 1.2 kb glucose isomerase gene, but there...

In order to obtain an excellent Streptomyces host, regarding two glucose isomerase(GI) deficient strains with thiostrepton resistance named M1033XW78 and M1033XW194 as starting strains, one strain named M53 which was sensitive to thiostrepton. The glucose isomerase deficient strains was chosen by liquid culture, photocopy and negative filtration. The analysis of PCR amplification, DNA sequencing and restriction enzyme digestion demonstrated that the chromosome of M53 had 1.2 kb glucose isomerase gene, but there were seventeen mutant nucleotides from 345 bp to 1 095 bp. It was concluded that low frequency point mutation appeared within the GI gene in the process of chromosome homologous recombination. Enzyme activity assay and SDS-PAGE analysis indicated that the mutant gene did not express 42.5 kD protein and had no enzyme activity. Therefore, it might provide enough expressive spaces when M53 was used as a host. A series of transforming tests also proved that M53 was likely to be a new Streptomyces recipient.

为了发展优良的链霉菌宿主系统 ,以带有硫链丝菌素抗性的同源重组葡萄糖异构酶 (GI)缺陷型菌株M10 33XW78,M10 33XW194为出发菌株 ,利用摇瓶、影印和负筛的方法 ,获得一株既无GI活性又对硫链丝菌素敏感的回复菌株 ,命名为淀粉酶链霉菌M5 3.通过染色体PCR检测、酶切图谱鉴定、序列分析等方法 ,确认M5 3含有和M10 33一致的 1 2kb葡萄糖异构酶基因 ,但在结构基因345~ 10 95bp片段内有 17个碱基发生突变 .这说明在染色体内自发同源重组过程中 ,有低频率的突变位点引入 .酶活力测定和SDS PAGE分析表明 ,该突变的GI基因不表达 4 2 5KD葡萄糖异构酶 ,这为M5 3菌株发展成为优良的链霉菌宿主提供了足够的表达空间 .一系列的转化实验也证明了M5 3菌株很可能是一种新型链霉菌克隆受体

Achieving splendid achievement in the 20~(th) century,the local colour novels still contain a lot of pities.What is most is to expose the sensitive utility and creative conception,one-way angle of the theme and straightforward way of image, limitation of view and single technique,etc.In the future development,the local color novel is to face different challenges.The modernized process makes it lose the appreciation of epochs;commercial pressure has it in trouble;the fewer authors and readers let it in an embarrassing...

Achieving splendid achievement in the 20~(th) century,the local colour novels still contain a lot of pities.What is most is to expose the sensitive utility and creative conception,one-way angle of the theme and straightforward way of image, limitation of view and single technique,etc.In the future development,the local color novel is to face different challenges.The modernized process makes it lose the appreciation of epochs;commercial pressure has it in trouble;the fewer authors and readers let it in an embarrassing situation.But it is well-known that opportunity and challenge both exist.The hardness and burden of the old local color in China's modernization process also offer rich materials and expressive space for local color novels.The pluralism of local color novels in the late of 20~(th)century indicates the advancing prospect in part.The outstanding lireral tradition formed in the last century is of course to one of important materials against crisis.Thus it is beliceved that the local color novel will have boundless prospects and harvest yield.

20世纪乡土小说成就辉煌,但也存在着种种遗憾:主要表现为观念的功利性与创作的概念化、主题的单向度与形象的平面化及视野的封闭性与手法的单一性等。在未来的发展中,乡土小说必须面对多种挑战:现代化进程的加快使之逐渐失去时代的垂青;商业主义的压力使之处境艰难;作者队伍的枯萎与读者的疏离使之日趋尴尬……但机遇与挑战并存,古老乡土中国现代化进程的艰难与沉重仍为乡土小说提供着丰厚的题材资源与表现空间,20世纪末乡土小说的多元发展格局已部分地预示出其发展前景,上世纪形成的优秀文学传统也必将是其反抗危机的重要资源之一……因而我们有理由相信,未来的乡土小说会前途广阔、收获丰硕。

 
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