The differences in exon 2 of MTNR1A gene between Jining grey goats and Liaoning cashmere goats consisted of eleven nucleotide changes(A52G,T232C,T253C,A256G,T358G,T410A,A414T,C424T,A554G,T559C and C589A),and the homology of nucleotide sequence between them was 98.7%.
The differences in exon 2 of MTNR1A gene between Small Tail Han sheep and Dorset sheep consisted of five nucleotide changes (C329T, G355T, C566T, C580A and A675G), and homology of nucleotide sequence was 99.4%.
Methods:PCR SSP was used to amplify RhD gene exons 2,3,4,5,6,7,9,10 and intron 4 as well as RhCE gene intron 4 in 80 RhD negative blood donors which were determined by routine serological method.
These PCR methods amplified RHD gene at exons 2、3、4、5、6、7、9、10 and at introm 4 and RHCE gene at intron 4.Results:RhD negative phenotypes consisted of 4 CCee,15 Ccee,14 ccee and 1 ccEe.
Three pairs of primers were designed according to known DNA sequences tyrosinase in human and cattle, and exons 2, 3 and 4 were amplified of DNA sequences of tyrosinase in pig, which were 210, 135 and 182 bp in length.
Methods:Four parts of ODC gene which were extron2,extron3,extron6-10,extron11-12 were amplified from hepatic cancer cell by RT-PCR and then reversely subcloned into pcDNA3.1 vector.
p16INK4a gene extron 1 and extron 2 inactivation was detected by PCR for gene deletion, PCR-SSCP for gene mutation, and PCR-restriction enzyme assay for gene methylation respectively.
NDK/Nm23-H2interacted with FMRP exon1-12,as well as FMRP isoforms without exon12,and exons14-17.NDK/Nm23-H2couldn' t interact with FMRP exon1-6and exon2-7fragments.
Three plant binary expression vectors pNAR501,pNAR502 and pNAR503 were constructed which carried exon 2-exon 3,5′partial deletion exon 1 and 5′partial deletion exon 1-exon 2-exon 3 of Pib gene driven by 35S separately.
case 2 carrying compound heterozygous point mutations of 527A→T and 1714G→C which caused heterozygous amino acid changes of D176V and V572L,respectively.
The variation of the exon 2 of the major histo-compatibility complex (MHC) class II gene DRB locus in three feline species were examined on clouded leopard (Neofelis nebulosa), leopard (Panthera pardus) and Amur tiger (Panthera tigris altaica).
The ratio of nonsynonymous substitutions (dN) was much higher than that of synonymous substitutions (dS), which indicated that balancing selection probably maintain the variation of exon 2.
The variation of the exon 2 of the major histo-compatibility complex (MHC) class II gene DRB locus in three feline species were examined on clouded leopard (Neofelis nebulosa), leopard (Panthera pardus) and Amur tiger (Panthera tigris altaica).
The ratio of nonsynonymous substitutions (dN) was much higher than that of synonymous substitutions (dS), which indicated that balancing selection probably maintain the variation of exon 2.
Exon 3 termination mutation of phenylalanine hydroxylase (PAH) gene, the only identified one causing classical phenylketonuria (PKU) in Chinese, was detected in fourteen PKU children from Xi'an. The genomic DNA from these patients was amplified by polymerase chain reaction(PCR) and dot hybridied with specific oligonucleotide probes. This mutation is not present in any of these affected children, which indicates that phenylketonuria in Chinese may be caused by other mutations in phenylalanine hydroxylase loc...
Applying recombinant DNA techni- ques,the structures of red pigment gene(RPG)and green pigment gene(GPG)were analyzed for 43 pa- tients with protan or deutan(including 3 females),4 normal reladves and 3 carriers out of 3 families,as well as 11 normal controls.Abnormality of RPG was detected in all 19 protan and that of GPG was found in 14 out of 24 deutan.In about 80%(32/40) of protan and deutan the changing of exon 5 for RPG or GPG was discovered.In protan the normal RPG was replaced by a 5' red —3' green ...
In this paper,we analyse Xba I/FⅧ_(E22)RFLP in the population of Suzhou usinga intragenic factor Ⅷ cDNA probe FⅧ_(E22).The results show 9.6/4.8 K b poly-morphism,and their frequency are 0.36/0.64 respectively.The value of PIC is 0.46Based on this polymorphism,carrier detection of hemophilia A was performed.