助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   5 '-nucleotide acid 的翻译结果: 查询用时:0.009秒
图标索引 在分类学科中查询
所有学科
农作物
更多类别查询

图标索引 历史查询
 

-nucleotide acid
相关语句
  相似匹配句对
     5: kadsuric acid.
     5:Kadsuric acidCOMP.
短句来源
     Isovanillic acid (5);
     异香草酸(Isovanillicacid ,即3 -Hydroxy- 4 - methoxy benzoicacid) (5 ) ;
短句来源
     Synthesis of 5-Fluoronicotinic Acid
     5-氟烟酸的合成
短句来源
     4-hydroxybenzoic acid (5);
     对羟基苯甲酸(5);
短句来源
     Synthesis of 5-Nitrovanillic Acid
     5-硝基香兰酸的合成
短句来源
查询“5 '-nucleotide acid”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
没有找到相关例句


The NDV F 46 E 9 and LaSotaE 4 strains were replicated in SPF chicken embyros, the virus in the allantoic fluid purified, and the virus RNA extracted. One pair of primers both 27b in length have been designed for RT PCR, which respectively correspond to the 45 ̄63 and 486 ̄504 nucleotide acids of the NDV Fc gene. The Fc genes of the two strains were amplified and cloned directionally into pUC18 between the Eco RI and Sal Ⅰ cleavage sites, getting the two Fc gene clones of pUCF 46 Fc and pUCLaFc....

The NDV F 46 E 9 and LaSotaE 4 strains were replicated in SPF chicken embyros, the virus in the allantoic fluid purified, and the virus RNA extracted. One pair of primers both 27b in length have been designed for RT PCR, which respectively correspond to the 45 ̄63 and 486 ̄504 nucleotide acids of the NDV Fc gene. The Fc genes of the two strains were amplified and cloned directionally into pUC18 between the Eco RI and Sal Ⅰ cleavage sites, getting the two Fc gene clones of pUCF 46 Fc and pUCLaFc. Using the methods of digestion with Eco RI/Sal Ⅰ and Pst Ⅰ, PCR, nucleic acid probe, the recombinants were identified, showing that the positions and oritentions were all correct. The Fc gene of the LaSotaE 4 was transfered into the expression vector pBV221 between the Eco RI and Sal Ⅰ sites, resulting in the recombinant pBVLaFc, and the clone was identified by restriction endonuclease analysis and PCR, proving that the foreign gene was in the correct position and oritention. The recombinant has been expressed in the E coli DH5α, and the expression product detected by SDS PAGE and Western blot methods. The results showed that a predicted 15 700 protein band, which could react with the NDV antiserum, suggesting that this peptide was the Fc gene expression product.

用SPF鸡胚繁殖新城疫病毒(NDV)F46E9株(强毒)、LaSotaE4株(弱毒),对病毒进行纯化,抽提RNA。用1对均为27个碱基的引物进行RT-PCR,扩增出了2个毒株的融合蛋白裂解位点(Fc)基因。将Fc基因定向克隆入pUC18的EcoRI和SalⅠ位点之间,获得2个毒株Fc基因克隆pUCF46Fc和pUCLaFc,用EcoRI/SalⅠ双酶切法、PstⅠ单酶切法、PCR法和核酸探针法对其进行了鉴定。将鉴定好的LaSotaE4Fc基因定向导入表达载体质粒pBV221,获得重组子pBVLaFc。PCR和内切酶酶切法鉴定表明,Fc插入的位置和方向都正确无误。用E.coliDH5α通过热诱导法进行了表达。用SDS-PAGE和Western-blot法检测了表达产物。结果表明,有1条能够与NDV多抗反应的特异条带,分子量约15700,与预期大小一致,说明是Fc基因的表达产物

In this paper,the allelic distributions of apo B gene 3'-variable number of tandemly repeat(VNTR)in Chinese Han and Mongolian nationalities were alalyzed by PCR techniqe. The nucleotide acids of HVE34 and HVE46 were sequencied by automatic Ma chine AB1373A Model.The results showed that 14 al leles in the samples of 300 Han individuals(164 cases of patients with coronary heart disease and 134 normal matched subjects) and 12 alleles in the samples of 61 Mongolian individuals. In Han nationalit y group,the...

In this paper,the allelic distributions of apo B gene 3'-variable number of tandemly repeat(VNTR)in Chinese Han and Mongolian nationalities were alalyzed by PCR techniqe. The nucleotide acids of HVE34 and HVE46 were sequencied by automatic Ma chine AB1373A Model.The results showed that 14 al leles in the samples of 300 Han individuals(164 cases of patients with coronary heart disease and 134 normal matched subjects) and 12 alleles in the samples of 61 Mongolian individuals. In Han nationalit y group,the most common allele is a repeat unit allele (HVE36),occupied 31% of the total alleles. HVE34 and HVE32 are 24%and 22%respectively. In comparison with Han nationality,the most common allele in Mongolian nationality group is HVE34,occupied 35.2%. HVE36and HVE32 are 28.7%and 13.9%respectively.Futhermore,we also found that the alleles of homozygote(80%)in Mongolian group are much higher than that(52%)of Han nationality group. No alleles less than HVE30 was found in the Mongolian group. The amplication products of homozygotes of HVE 34 and HVE46 alleles were directly aequenced. It was found that the 3' hypervariable region apo B gene consists primarily of a 15 bp basic repeat unit called X and Y sequence and tandemly repeat in an X-Y Order. The ATrich region also contains variants of X and Y sequences resulted from C or G for A substitution. The length of different alleles was ditermined by the numbers of X and Y copies. The HVE polymorphism of apo B gene not only resides in the variable numbers of repeat units,but also resides in the variation in the oligonucleotide sequence within the repeat units as well as the variation in the arrangement of the repeat.

载脂蛋白B基因3’末端下游有一个高可变串连重复序列(variablenumberoftandemlyrepeat,VNTR),也叫高可变区。每一个串连重复序列长度在15bp左右,富含A、T两个核苷酸,不同的串连重复序列拷贝数构成了等位基因的高度多态性。本文应用PCR技术分析了汉族和蒙古族人群载脂蛋白B基因3'VNTR等位基因的分布和频率;并测定了等位基因HVE34和HVE46的核苷酸序列。在汉族人群(164例冠心病患者,136例正常人)中共分离到14个3’VNTR等位基因,以HVE36的分布频率(31.4%)最高,其次是HVE34,约占24.0%,而从61例蒙古族正常人本课题系攀登计划”与“

The cDNA fragments amplified through nested RT-PCR from the small hydrophobic (SH) protein gene and its flanking region of the five wild mumps viruses and Enders strain were subjected to singlestrand conformation polymorphism (SSCP) analysis by using silver staining. These five wild isolates could be divided into three SSCP patterns and they were differentiated from the Enders strain. Two wild strains which have 3.4% heterology of the nucleotide acid sequence could be distinguished by the method. The...

The cDNA fragments amplified through nested RT-PCR from the small hydrophobic (SH) protein gene and its flanking region of the five wild mumps viruses and Enders strain were subjected to singlestrand conformation polymorphism (SSCP) analysis by using silver staining. These five wild isolates could be divided into three SSCP patterns and they were differentiated from the Enders strain. Two wild strains which have 3.4% heterology of the nucleotide acid sequence could be distinguished by the method. The results were well correlated with sequence analysis of the SH gene segments, indicating high applicability of the silver staining SSCP analysis for differentiation of mumps virus strains. Furthermore, the method, which is not only quick, sensitive, safe and reliable, but also reproducible and inexpensive, can be used in the molecular epidemiology study of mumps viruses.

用腮腺炎病毒小疏水蛋白(SH)基因及其旁侧区的逆转录(RT)套式聚合酶链反应(N-PCR)产物进行银染单链构象多态性(SSCP)分析,建立了较适的样品变性和聚丙烯酰胺凝胶电泳(PAGE)条件。根据单链电泳模式可将所测6株腮腺炎病毒分为四种SSCP模式,3株兰州野毒株Wlz1,Wlz2,Wlz3呈现一种模式,这3株病毒的SH基因及其旁侧区cDNA序列之间平均差异仅为0.96%;两株上海野毒株Wsh1和Wsh2株的相应序列之间差异为4%,呈现另两种模式。Enders株呈现一种与野毒株差别较大的特殊模式。3株兰州野毒与2株上海野毒相应区域序列之间差异为3.4%~4.5%,故本法可区别SH基因及其旁侧区cDNA序列之间差异为3.4%的野毒株,且SSCP模式的相近程度与相应区域中核苷酸序列同源性大小一致。此法可用于腮腺炎病毒株分子特性和遣传变异的初步鉴定和分子流行病学研究

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关5 '-nucleotide acid的内容
在知识搜索中查有关5 '-nucleotide acid的内容
在数字搜索中查有关5 '-nucleotide acid的内容
在概念知识元中查有关5 '-nucleotide acid的内容
在学术趋势中查有关5 '-nucleotide acid的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社