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glycoprotein h gene
相关语句
  糖蛋白h基因
     The sequencing and expression of glycoprotein H gene of human berpesirus-6
     人6型疱疹病毒糖蛋白H基因测序与表达
短句来源
     Cloning Sequencing and Structrual Analysis of the Glycoprotein H Gene of A Wild Chinese Pseudorabies Virus Strain
     伪狂犬病毒糖蛋白H基因片段的克隆、序列测定和分析
短句来源
     With pured PRV genome DNA as template, the part region of glycoprotein H gene and the upstream tk gene located with genomic BamHI fragments 11 and 16 was amplified by PCR, and its nucleotide sequence was determined by the dideoxy\|mediated chain termination method.
     对伪狂犬病毒湖北地方株 (PRV HB株 )糖蛋白 H基因片段进行了扩增、克隆、序列测定和分析 ,比较了该序列与 PRV Ka株及 NIA- 3株三者之间的同源性 .
短句来源
  “glycoprotein h gene”译为未确定词的双语例句
     HHV -6 , human herpesvirus-6 , is one of members of herpesviruses. By sequencing of BamH I H fragment of HHV-6 using random ditdeoxynucleotide chain termination method ,glycoprotein H gene was identified. It was similar to that of HCMV in 46.139% .
     用随机双脱氧核苷酸链末端终止法对人6型疱疹病毒(HHV-6)糖蛋白H(gH)基因进行DNA序列测定,找出开读框架,将其大部分插入到质粒pET3CP并分别转染大肠杆菌株BL21、PLYSS和PLYSE。
短句来源
  相似匹配句对
     Advances in Inhibitor of P-Glycoprotein
     P-糖蛋白抑制剂的研究进展
短句来源
     P-glycoprotein and intractable epilepsy
     P-糖蛋白与难治性癫痫的关系
短句来源
     The construction and function of Glycoprotein
     糖蛋白的结构与功能
短句来源
     P-GLYCOPROTEIN AND VOLUME REGULATION
     P-gp和细胞容积调节
短句来源
     The gene of the glycoprotein g13 of EHV- I was sequenced.
     本文分析了其膜糖蛋白g13基因的碱基序列,并由此推导其相应的氨基酸序列。
短句来源
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  glycoprotein h gene
Identification and characterization of the guinea-pig cytomegalovirus glycoprotein H gene
      


HHV -6 , human herpesvirus-6 , is one of members of herpesviruses. By sequencing of BamH I H fragment of HHV-6 using random ditdeoxynucleotide chain termination method ,glycoprotein H gene was identified. It was similar to that of HCMV in 46.139% . The open reading frame of gH was inserted into pET 3CP then transfered to E. coli ,BL21 ,PLYS S and PLYS E strains. The expression of gH was seen by 35S-Methionine incorporation assay.

用随机双脱氧核苷酸链末端终止法对人6型疱疹病毒(HHV-6)糖蛋白H(gH)基因进行DNA序列测定,找出开读框架,将其大部分插入到质粒pET3CP并分别转染大肠杆菌株BL21、PLYSS和PLYSE。35S甲硫氨基酸掺入试验表明,带有部分HHV-6gH基因片段的pET3CP质粒,可在上述大肠杆菌株表达,分子量约为37×10~3。

With pured PRV genome DNA as template, the part region of glycoprotein H gene and the upstream tk gene located with genomic BamHI fragments 11 and 16 was amplified by PCR, and its nucleotide sequence was determined by the dideoxy\|mediated chain termination method. A analysis of the nucleotide sequence and its deduced amino acid seqnence was performed with computer programs. The results revealed that the region of DNA sequenced was 1396bp and had the content of (G+C) 75.6%. The nucleotide sequence...

With pured PRV genome DNA as template, the part region of glycoprotein H gene and the upstream tk gene located with genomic BamHI fragments 11 and 16 was amplified by PCR, and its nucleotide sequence was determined by the dideoxy\|mediated chain termination method. A analysis of the nucleotide sequence and its deduced amino acid seqnence was performed with computer programs. The results revealed that the region of DNA sequenced was 1396bp and had the content of (G+C) 75.6%. The nucleotide sequence comparison of gH gene showed that the homology of the nucleotide sequence of PRV HB strain with PRV Ka strain and NIA\|3 strain was both 99.6%. The deduced amino acid sequence of gH polypeptide exhibited a strongly hydrophobic region present at the N\|terminus at position 1~30 amino acids, and exhibited features typical for eucaroytic signal sequences.

对伪狂犬病毒湖北地方株 (PRV HB株 )糖蛋白 H基因片段进行了扩增、克隆、序列测定和分析 ,比较了该序列与 PRV Ka株及 NIA- 3株三者之间的同源性 .结果显示 ,克隆片段长 1396 bp,G+C含量 75 .6 % ,包括糖蛋白 H (g H ) N端 2 83个氨基酸编码区及上游调控序列和胸苷激酶 (TK) C端 136个氨基酸编码区 .g H基因ORF上游调控区存在有 TATA框和 CAT框的真核启动子特征结构 .PRV HB株 g H基因片段序列与 PRV Ka株和 NIA- 3株相应序列的核苷酸同源性相同 ,为 99.6 % .推导的 g H多肽 N端第 1~ 30位氨基酸组成的肽段富含疏水性氨基酸 ,具有真核信号肽的序列特征 .

 
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