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collagen induced arthritis rat model
相关语句
  大鼠关节炎模型
     In order to study the mechanism of the effect of Wenjing Mixture on rheumatoid arthritis treatment, we observed the index of swollen joints, the changes of level of IL-1β and TNF-asecreted by joint synovial cells effected by serum containing medicine and in synovial fluid of articular cavity in collagen induced arthritis rat model, by which, medicine Tripterygium glycosides(TG) was used as positive control.
     采用Ⅱ型胶原诱导大鼠关节炎模型,以雷公藤多甙作对照,通过对大鼠的关节肿胀指数观察及检测关节滑液及问荆合剂含药血清对滑膜细胞细胞因子TNF-α、IL—1β水平的影响,探讨问荆合剂治疗类风湿关节炎的部分作用机理。
  相似匹配句对
     Establishment of animal model with collagen-induced arthritis
     鸡Ⅱ型胶原诱导的类风湿关节炎大鼠模型的建立
短句来源
     Inhibition of Celastrus extract on the model in type Ⅱ collagen-induced arthritis in rat
     南蛇藤提取物对大鼠胶原诱导性关节炎(CIA)的抑制作用
     Rheumatoid Arthritis Model in Rat Induced by Type Ⅱ Collagen and Freund's Complete
     Ⅱ型胶原蛋白与弗氏完全佐剂大鼠关节炎模型的建立和比较
短句来源
     T Lymphocyte and Collagen-induced Arthritis
     T淋巴细胞与胶原诱导性关节炎
短句来源
     The rat model was induced by alloxan.
     动物模型用四氧嘧啶塑造。
短句来源
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Objective:To establish collagen-induced arthritis rat model similar to RA pathogenesis, compare and conduct different proteomic analysis between normal rats and CIA rats synovium, and thus identify proteins related to CIA rat pathological synovium, as well as better understand pathogenesis of RA.Methods:Collagen-induced arthritis rat model with cow Ⅱ collagen and complete Freud adjuvant was established; synovial proteins were...

Objective:To establish collagen-induced arthritis rat model similar to RA pathogenesis, compare and conduct different proteomic analysis between normal rats and CIA rats synovium, and thus identify proteins related to CIA rat pathological synovium, as well as better understand pathogenesis of RA.Methods:Collagen-induced arthritis rat model with cow Ⅱ collagen and complete Freud adjuvant was established; synovial proteins were extracted, and two dimensional electrophoresis was used to separate total proteins from normal and model rat synovium. Having been stained, proteins were analyzed by PDquest software. Some selected protein spots were identified by peptide mass fingerprint(PMF) based on Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) and database.Results:CIA rat model was established successfully. 2-DE patterns with high resolution and reproducibility from rat synovial proteins was obtained. The average spots of normal, 25, 35 and 45-day rat synovial proteins were 1 070±41,1 049±22,1 079±44 and 1 088±39. 20 protein spots chosen randomly from normal and model groups were identified by PMF, some of which were related to cell metabolism, signal transduction and so forth.Conclusion:Well resolved and reproducible 2-DE profiles of normal and CIA rat synovium in different stages have been established, and some differently expressed proteins concerning CIA rat synovitis has been identified. The results may provide a basis for further research of RA pathogenesis.

目的类风湿关节炎(Rheumatoid arthritis,RA)是一个多基因多因素参与的病理过程。实验中通过建立与RA发病机制相似的胶原诱导性关节炎(Collagen-induced arthritis,CIA)大鼠动物模型,运用蛋白质组技术对正常大鼠和CIA大鼠模型不同时间点滑膜组织的蛋白质点进行比较分析,为鉴定CIA大鼠滑膜病变相关蛋白质及研究RA发病机制奠定基础。方法用牛Ⅱ型胶原和完全福氏佐剂建立CIA大鼠模型,采用一步抽提法抽提滑膜蛋白质,运用固相pH梯度双向凝胶电泳分离各组大鼠滑膜组织的总蛋白质,用PDquest软件分析图谱,比较差异蛋白质,应用基质辅助激光解吸电离飞行时间质谱(Matrix-assisted laser desorption/ionization time-of-flightmass spectrometry,MALDI-TOF-MS)得到相应肽质指纹图谱,用Mascot查询软件搜索数据库,鉴定部分差异蛋白质。结果成功建立了CIA大鼠模型,获得分辨率较高、重复性较好的大鼠滑膜组织双向电泳凝胶图谱。正常组与25、35和45天模型组大鼠滑膜组织凝胶图谱的平均蛋白质点数分别为1070±...

目的类风湿关节炎(Rheumatoid arthritis,RA)是一个多基因多因素参与的病理过程。实验中通过建立与RA发病机制相似的胶原诱导性关节炎(Collagen-induced arthritis,CIA)大鼠动物模型,运用蛋白质组技术对正常大鼠和CIA大鼠模型不同时间点滑膜组织的蛋白质点进行比较分析,为鉴定CIA大鼠滑膜病变相关蛋白质及研究RA发病机制奠定基础。方法用牛Ⅱ型胶原和完全福氏佐剂建立CIA大鼠模型,采用一步抽提法抽提滑膜蛋白质,运用固相pH梯度双向凝胶电泳分离各组大鼠滑膜组织的总蛋白质,用PDquest软件分析图谱,比较差异蛋白质,应用基质辅助激光解吸电离飞行时间质谱(Matrix-assisted laser desorption/ionization time-of-flightmass spectrometry,MALDI-TOF-MS)得到相应肽质指纹图谱,用Mascot查询软件搜索数据库,鉴定部分差异蛋白质。结果成功建立了CIA大鼠模型,获得分辨率较高、重复性较好的大鼠滑膜组织双向电泳凝胶图谱。正常组与25、35和45天模型组大鼠滑膜组织凝胶图谱的平均蛋白质点数分别为1070±41、1049±22、1079±44和1088±39。在4个组均有表达差异的蛋白质点中随机取20个点进行肽质指纹图分析,鉴定了部分与细胞代谢、信号转导及结构相关的差异表达蛋白质。结论建立了分辨率较高且重复性较好的正常大鼠与CIA大鼠不同时间点滑膜组织的双向凝胶电泳图谱,鉴定了一些与CIA大鼠滑膜病变相关的差异表达蛋白质,为识别RA病变相关蛋白质及推断RA病变过程奠定基础。

>=In order to study the mechanism of the effect of Wenjing Mixture on rheumatoid arthritis treatment, we observed the index of swollen joints, the changes of level of IL-1β and TNF-asecreted by joint synovial cells effected by serum containing medicine and in synovial fluid of articular cavity in collagen induced arthritis rat model, by which, medicine Tripterygium glycosides(TG) was used as positive control. The results showed that the index of swollen joints, the level of IL-1β and TNF-asecreted by joint...

>=In order to study the mechanism of the effect of Wenjing Mixture on rheumatoid arthritis treatment, we observed the index of swollen joints, the changes of level of IL-1β and TNF-asecreted by joint synovial cells effected by serum containing medicine and in synovial fluid of articular cavity in collagen induced arthritis rat model, by which, medicine Tripterygium glycosides(TG) was used as positive control. The results showed that the index of swollen joints, the level of IL-1β and TNF-asecreted by joint synovial cells and the level in synovial fluid of articular cavity were significantly decreased. These results suggested that Wenjing Mixture can inhibit the product of IL-1β, TNF-αby joint synovial cells, which may be one of the mechanism of the effect of Wenjing Mixture on rheumatoid arthritis treatment.

采用Ⅱ型胶原诱导大鼠关节炎模型,以雷公藤多甙作对照,通过对大鼠的关节肿胀指数观察及检测关节滑液及问荆合剂含药血清对滑膜细胞细胞因子TNF-α、IL—1β水平的影响,探讨问荆合剂治疗类风湿关节炎的部分作用机理。结果发现问荆合剂能显著减轻大鼠关节肿胀指数、不同程度地抑制大鼠关节滑液和滑膜细胞分泌的炎性细胞因子TNF-α、IL—1β的水平。提示问荆合剂对Ⅱ型胶原诱导的大鼠关节炎具有一定抑制作用,可通过抑制关节滑膜分泌的炎性细胞因子TNF-α、 IL-1β的水平,从而减轻关节滑膜的炎症,可能是其治疗类风湿关节炎的作用机理之一。

 
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