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esi mass spectra
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     The ESI mass spectra demonstrate that the degree of complexes formation depends on the concentration ratio of baicalin: Al 3+ single charged ions corresponding to [AlR 1R 2(L)] + and [Al(L)_2] +, where L=[M-H] -,R 1=CH_3OH,R 2=CH_3O, have been observed, and the possible structures of various complexes and corresponding fragmentation mechanisms have been proposed.
     研究结果表明,黄芩苷与铝离子主要形成比较稳定的1∶1和1∶2配合物,分别为[AlR1R2L]+和[AlL]+,其中L=[M-H]-,R1=CHOH,R2=CHO。
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     The differ- ences of the ESI mass spectra suggest the structural information of polyamides, and the two isomers were distinguished by ESI-MS.
     利用这些碎裂特征,可以得到聚酰胺丰富的结构信息和区分它们的两种同分异构体.
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     At the same time, there were addition ion in the ESI-MS spectra.
     当化合物分子量小于 10 0 0时 ,则ESI -MS谱出现[2M +Na]+ 的系列离子。
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     Enterprise's Sustainable Innovation (ESI) is important for the innovation theory and practice.
     企业持续创新是当代十分重要的创新理论和实践问题。
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     Some important tables in ESI and their brief explanation are also given.
     文中还列出了ESI中一些重要的图表并作了简要说明。
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     The synthetic mutant was identified by Edman degradation and ESI (electrospray ionization) mass spectrometry.
     合成的突变体用 Edman降解和电喷雾质谱法进行鉴定 .
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     ESI MS showed that the relative molecular mass of hGLP 1 was the same as expected.
     用 ESI质谱鉴定 h GL P- 1相对分子质量与理论值一致。
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  esi mass spectra
and electrospray ionisation (ESI) mass spectra and by t.g.a.
      
ESI mass spectra of solutions containing [Au(S2O3)2]3- or [Au(CN)2]-, and HSA, provided evidence for the formation of protein complexes in which intact gold molecules were non-covalently bound.
      
Their ESI mass spectra, by direct infusion, gave average molecular masses comparable to those previously obtained for aquatic humic materials.
      
The ESI mass spectra showed a deprotonated molecule [M-H]-, which was chosen as precursor ion.
      
All heterocyclic amines tested in this study gave very simple ESI mass spectra and strong signals corresponding to [M+H]+ were observed for all heterocyclic amines except for Glu-P-1 and Glu-P-2 ([M+NH4-H2O]+ ions were observed from these amines).
      
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ESI-MS is a soft ionization MS method newly developed, which can detect a sort of compounds existing as ions in solution. According to the capability of accepting electron of fullerenes, the mixtures of fullerenes in toluene may be determined directly by ESI-MS without pre-ionization. The negative-ion ESI mass spectra indicate that there have C60, C70, C76, C78, C80, C82, C84, C86, C88 , C90 , C92, C94 and C96 in the mixture of fullerenes. The same results were obtained by pre - ionization...

ESI-MS is a soft ionization MS method newly developed, which can detect a sort of compounds existing as ions in solution. According to the capability of accepting electron of fullerenes, the mixtures of fullerenes in toluene may be determined directly by ESI-MS without pre-ionization. The negative-ion ESI mass spectra indicate that there have C60, C70, C76, C78, C80, C82, C84, C86, C88 , C90 , C92, C94 and C96 in the mixture of fullerenes. The same results were obtained by pre - ionization using potassium - naphthalide as reducing agent. It indicated that the ability of accepting electron of fullerenes in the process of ESI - MS is the same as in the reaction process using potassium - naphthalide as reducing agent.

电喷雾电离是新近发展的一种质谱技术,适用于分析在溶液中呈离子状态的物质,具有无碎片的特点.本文根据富勒烯分子的电子亲和性能,将富勒烯混合物的甲苯溶液直接进行电喷雾质谱分析,检出了C_(60),C_(70),C_(76),C_(78),C_(80),C_(82),C_(84),C_(86),C_(88),C_(90),C_(92),C_(94)和C_(96)等的负离子峰.并用萘钾作还原剂,将富勒烯混合物预先还原,制得富勒烯负离子溶液后再作电喷雾质谱分析,得到相同的结果.表明各类富勒烯分子在电喷雾过程中俘获电子的能力与被萘钾还原的能力相一致.

The positive and negative ion electrospray ionization mass spectra of β cyclodextrin amino acids complexes in NH 4Ac buffer have been reported in this paper. Compared with positive ion ESI mass spectra of β cyclodextrin amino acids complexes under the same condition, negative ion mass spectra obtained for inclusion complexes of β cyclodextrin (CD) with tyrosine, phenylalanine and tryptophan, respectively, were completely dominated by deprotonated complex ions and CD-H -...

The positive and negative ion electrospray ionization mass spectra of β cyclodextrin amino acids complexes in NH 4Ac buffer have been reported in this paper. Compared with positive ion ESI mass spectra of β cyclodextrin amino acids complexes under the same condition, negative ion mass spectra obtained for inclusion complexes of β cyclodextrin (CD) with tyrosine, phenylalanine and tryptophan, respectively, were completely dominated by deprotonated complex ions and CD-H - ion which is the only daughter ion in collision induced dissociation (CID) experiment of deprotonated complexes. The results indicated that the charged position for protonated and deprotonated complexes is different from each other. In addition, two complex ions for the same complex have similarly relative dissociation energies, which are higher than that of CD+NH 4 +, indicating that complexes observed in gas phase are not electrostatic adducts at all but complexes formed by hydrogen bonds.

近年来,电喷雾质谱作为“软电离”质谱新技术广泛应用于生命科学领域[1,2],1991年Ganem等[3]首次利用电喷雾质谱(ESI-MS)对溶液中特异性免疫蛋白质-药物复合物进行了研究.目前生物酶-底物/抑制剂、DNA-药物、蛋白质-配体及生物大分子...

AIM: To purify and characterize a potassium channel blocker ( Bm P 3) from the venom of Chinese scorpion Buthus martensii Karsch. METHODS: ① Purification was carried out by gel filtration, cation exchange, and reversed phase chromatographies. N terminal was directly sequenced by double coupling manual method. Molecular weight was determined on an electrospray ionization mass spectrometer. Amino acid composition was analyzed after acidic hydrolysis for 20 h in HCl 6 mol·L -1 at 110 ℃. ②Toxicity...

AIM: To purify and characterize a potassium channel blocker ( Bm P 3) from the venom of Chinese scorpion Buthus martensii Karsch. METHODS: ① Purification was carried out by gel filtration, cation exchange, and reversed phase chromatographies. N terminal was directly sequenced by double coupling manual method. Molecular weight was determined on an electrospray ionization mass spectrometer. Amino acid composition was analyzed after acidic hydrolysis for 20 h in HCl 6 mol·L -1 at 110 ℃. ②Toxicity tests were conducted in mice and cockroaches. ③The inhibitory effects of Bm P 3 on K + channels were tested in acutely dissociated rat hippocampal pyramidal neurons using whole cell patch clamp configuration. RESULTS: ① A pure peptide ( Bm P 3, 8 1 mg) was obtained, about 0.08 % of total proteins of the venom. The N terminal sequences were VGCEE and the molecular weight was 2938 in ESI mass spectra. ② No death occurred at the dosage of 200 μg in mice and 8 μg in cockroaches. ③The peptide at 10 μmol·L -1 reduced the peak outward K + currents by 63 %±4 % in vitro . CONCLUSION: Bm P 3 inhibited K + channels.

AK+channelblockingpeptidefromvenomofChinesescorpionButhusmartensiKarsch1WUGong,WEIDongSheng2,HEFaHu,HUGuoYuan2,WUHouMing...

 
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