助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   rabies virus glycoprotein gene 的翻译结果: 查询用时:0.163秒
图标索引 在分类学科中查询
所有学科
生物学
基础医学
更多类别查询

图标索引 历史查询
 

rabies virus glycoprotein gene
相关语句
  狂犬病毒糖蛋白基因
     STUDY ON BIOLOGICAL CHARACTERISTICS OF RECOMBINANT VACCINIA VIRUS CONTAING RABIES VIRUS GLYCOPROTEIN GENE
     含狂犬病毒糖蛋白基因的重组痘苗病毒(RTTVV-3)传代后生物学特性研究
短句来源
  狂犬病病毒糖蛋白基因
     Expression of Rabies Virus Glycoprotein Gene in E. coli
     狂犬病病毒糖蛋白基因在原核细胞中的表达
短句来源
     With flank sequence of HA gene of vaccinia virus, four eucaryotic expression plasmids containing cDNA of rabies virus glycoprotein gene under the control of different hybrid promoters of pox virus were constructed and transfected into BHK 21 cells infected with wild type vaccinia virus.
     以血凝素(HA)基因为侧翼,将狂犬病病毒糖蛋白基因cDNA置于不同类型的痘苗病毒复合启动子下游,构建了4种表达质粒。
短句来源
  “rabies virus glycoprotein gene”译为未确定词的双语例句
     A recombinant plasmid comprising adenovirus genome with rabies virus glycoprotein gene in E1 region was constructed by homologous recombination.
     利用大肠杆菌内质粒间同源重组的方法,将狂犬病病毒G基因插入腺病毒基因组的E1基因区,构建了带有狂犬病病毒G基因的重组腺病毒质粒。
短句来源
     The rabies virus glycoprotein gene was cloned into the Bam HI site ofhigh level expressing vectors-pET-17b and pET-17b2. And pET-17b2 was reconstructed from pET-17b by Nde Ⅰ- Sac Ⅱ Ⅰ. Two recombinant plasmids,pET-17RVgpand pET-17b2RVgp were gotten. They were transferred into expressing host cells E。
     将狂犬病病毒糖蛋白(RVgp)基因BglⅡ片段(1675bp)分别正向插入到原核高效表达载体pET-17b和pET-17b2(用SacⅠ-NdeⅠ缺失掉pET-17b60bp含起始密码子ATG小片段)的BamHⅠ切点,构建重组质粒pET-17bRVgp和pET-17b2RVgp。
短句来源
     he recombinant vaccinia virus(VVM11KRG strain)contains rabies virus glycoprotein gene(RVGG) which was inserted into the Hind ⅢM fragment of vaccinia virus(V.V)genome.
     对表达狂犬病毒糖蛋白的重组痘苗病毒VVM11KRG株生物学性质进行了研究,该重组病毒的特点是:(1)糖蛋白基因插入到痘苗病毒天坛株基因组HindⅢM片段中;
短句来源
     HA gene of vaccinia virus is an unnecessary region for virus replication It was used as a flank to construct 12 recombinant vaccinia viruses,in which HIV-1 gag gene,HIV-1  gag-env  chimeric gene and Rabies virus glycoprotein gene were inserted into the downsteam of various types of pox virus promoter.
     以痘苗病毒HA基因为侧翼,在多种类型痘病毒)启动子的下游,插入HIV-1的gag基因及其上游序列、或HIV-1gag-env嵌合基因、或狂犬病病毒的糖蛋白基因,从而构建了12株重组痘苗病毒。
短句来源
     GOMPARATIVE STUDIES OF TWO METHODS TRANSFECTING RABIES VIRUS GLYCOPROTEIN GENE
     两种方法转染狂犬病毒糖蛋白基因的比较研究
短句来源
更多       
  相似匹配句对
     Rabies Virus Liposome Vaccine Research
     狂犬病毒脂质体疫苗的研究
短句来源
     Rabies virus antigen in Negri body
     Negri小体内的狂犬病毒抗原
短句来源
     Morphological and Morphogenetic Observation of Rabies Virus
     狂犬病病毒的形态和形态发生学观察
短句来源
     Expression of Rabies Virus Glycoprotein Gene in E. coli
     狂犬病病毒糖蛋白基因在原核细胞中的表达
短句来源
     Comparison and analysis of G gene of rabies virus M strain
     狂犬病病毒野毒株糖蛋白基因序列测定及分析比较
短句来源
查询“rabies virus glycoprotein gene”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  rabies virus glycoprotein gene
Protection from rabies by a vaccinia virus recombinant contain ing the rabies virus glycoprotein gene.
      


The rabies virus glycoprotein gene was cloned into the Bam HI site ofhigh level expressing vectors-pET-17b and pET-17b2. And pET-17b2 was reconstructed from pET-17b by Nde Ⅰ- Sac Ⅱ Ⅰ.Two recombinant plasmids,pET-17RVgpand pET-17b2RVgp were gotten.They were transferred into expressing host cells E。coli BL21 (DE3)and BL21(DE3)plysS strains respectively.Inducing with IPTG,theyall express rabies virus glycoprotein which is about 60 000 dalton in molecular weight bySDS-pAGE.Rabies virus...

The rabies virus glycoprotein gene was cloned into the Bam HI site ofhigh level expressing vectors-pET-17b and pET-17b2. And pET-17b2 was reconstructed from pET-17b by Nde Ⅰ- Sac Ⅱ Ⅰ.Two recombinant plasmids,pET-17RVgpand pET-17b2RVgp were gotten.They were transferred into expressing host cells E。coli BL21 (DE3)and BL21(DE3)plysS strains respectively.Inducing with IPTG,theyall express rabies virus glycoprotein which is about 60 000 dalton in molecular weight bySDS-pAGE.Rabies virus glycoprotein were identified with RVgp McAb by Western-blot.Thin-layer Chromatography-scanning indicated that the expressing quantity ofglycoprotein is about 10%~14%of the cell total protein.

将狂犬病病毒糖蛋白(RVgp)基因BglⅡ片段(1675bp)分别正向插入到原核高效表达载体pET-17b和pET-17b2(用SacⅠ-NdeⅠ缺失掉pET-17b60bp含起始密码子ATG小片段)的BamHⅠ切点,构建重组质粒pET-17bRVgp和pET-17b2RVgp。将其分别转化表达受体菌E.coliBL21(DE3)和E,coliBL21(DE3)plysS.IPTG诱导表达,菌体经超声波裂解处理后SDS-pAGE,染色,在分子量约60000处可见重组质粒表达的较宽的蛋白带,以抗RVgpMcAb进行Western-blot检测,表明该表达蛋白为RVgp。通过扫描显示,表达的RVgp占菌体总蛋白的10%~14%,其中pET-17b2RVgp在E。coliBL21(DE3)中的表达量最高。

he recombinant vaccinia virus(VVM11KRG strain)contains rabies virus glycoprotein gene(RVGG) which was inserted into the Hind ⅢM fragment of vaccinia virus(V.V)genome.TheRVGG was promoted by pll promoter of V.V.The VVM11KRG does not contain lac gene.Thestudy showed that its titer is higher than that of another recombinant vaccinia virus(VVTK11KRG strain)in chicken embryo cell and CV-1 cell.Its titer reaches the highest point inchicken embryo cell in the third day and in CV-1 cell in the...

he recombinant vaccinia virus(VVM11KRG strain)contains rabies virus glycoprotein gene(RVGG) which was inserted into the Hind ⅢM fragment of vaccinia virus(V.V)genome.TheRVGG was promoted by pll promoter of V.V.The VVM11KRG does not contain lac gene.Thestudy showed that its titer is higher than that of another recombinant vaccinia virus(VVTK11KRG strain)in chicken embryo cell and CV-1 cell.Its titer reaches the highest point inchicken embryo cell in the third day and in CV-1 cell in the second day after inoculation Its sta-bility is no difference comparing with parent strain at different temperatures.The virulence ofVVM11KRG is lower than that of vaccinia virus.Western blot and IFA proved that rabies virusG protein was expressed.Southern blot showed that the RVGG was inserted into Hind Ⅲ Mfragment of vaccinia virus.The fragment between glycoprotein gene and promoter was cloned toplasmid pGEM3 zf(-).The DNA was sequenced by Pharmacia LKB DNA sequencer.Thus weobtained a clear DNA reference data for the usage of recombinant vaccinia virus.

对表达狂犬病毒糖蛋白的重组痘苗病毒VVM11KRG株生物学性质进行了研究,该重组病毒的特点是:(1)糖蛋白基因插入到痘苗病毒天坛株基因组HindⅢM片段中;(2)启动子为痘苗病毒天坛株P11晚期启动子;(3)不含外源lac基因。该重组病毒在CV-1细胞和鸡胚细胞上繁殖滴渡略高于另一株重组痘苗病毒VVTK11KRG,在鸡胚细胞中繁殖滴度第三天达到最高,在CV-1细胞中第二天达到最高。温度稳定性与天坛株相比没有明显改变。重组病毒在家兔皮内的毒力比亲本株(天坛株)低。间接免疫荧光和Westernblot都证明了狂犬病毒糖蛋白有良好的表达。通过Southernblot证实糖蛋白基因准确地插入到HindⅢM片段中。重组痘苗病毒启动子与部分糖蛋白基因克隆到pGEM3zf(-)质粒上,对该段DNA序列分析表明不会产生移码和融合蛋白,从而为该重组病毒的使用提供了明确的基因背景资料。

With flank sequence of HA gene of vaccinia virus, four eucaryotic expression plasmids containing cDNA of rabies virus glycoprotein gene under the control of different hybrid promoters of pox virus were constructed and transfected into BHK 21 cells infected with wild type vaccinia virus. Four RVgp recombinant vaccinia viruses (rVV): vSFJ1 10RVgp, vSFJ2 16RVgp, vRJ1 10RVgp and vRJ2 10RVgp were screened and cloned by hemadsorption in BHK 21 cells, and further characterized...

With flank sequence of HA gene of vaccinia virus, four eucaryotic expression plasmids containing cDNA of rabies virus glycoprotein gene under the control of different hybrid promoters of pox virus were constructed and transfected into BHK 21 cells infected with wild type vaccinia virus. Four RVgp recombinant vaccinia viruses (rVV): vSFJ1 10RVgp, vSFJ2 16RVgp, vRJ1 10RVgp and vRJ2 10RVgp were screened and cloned by hemadsorption in BHK 21 cells, and further characterized with indirect immunofluorescence assay (IFA). IFA and Western blot demonstrated that the RVGP was fully expressed by all rVVs in BHK 21 cells. Western blot showed that the protein in supernatants of infected BHK 21 cells' lysate was able to react specifically with anti RVGP McAb, with it's molecular weight being 69 000. RVGP induced by vSFJ1 10RVgp accounted for the most quantity in the early stage of rVV infection and was as 2 fold as that of the others. In the late stage, vSFJ2 16RVgp was able to product the most output as 2 8 folds as that of the other rVVs. The results also indicated that the RVgp recombinant VVs could induce different titers of specific antibody in mice.

以血凝素(HA)基因为侧翼,将狂犬病病毒糖蛋白基因cDNA置于不同类型的痘苗病毒复合启动子下游,构建了4种表达质粒。重组表达质粒转染已感染WR株痘苗病毒的BHK21细胞,并通过鸡红细胞吸附试验,筛选、纯化出与表达质粒对应的4组HA-重组痘苗病毒:vSFJ1-10RVgp,vSFJ2-16RVgp,vRJ1-10RVgp,vRJ2-10RVgp。经间接免疫荧光试验(IFA)鉴定,从4组重组病毒中每组各鉴定出1株阳性重组病毒。Westernblot检测显示,重组病毒感染的BHK21细胞裂解物上清中有1种蛋白与抗RVGP的McAb发生特异反应,分子量为69000。在重组病毒感染早期,RVGP的表达量以vSFJ1-10RVgp最高;而在感染晚期,则以vSFJ2-16RVgp最高。4株重组病毒免疫小鼠,均能够不同程度地诱导小鼠产生抗RVGP特异性抗体。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关rabies virus glycoprotein gene的内容
在知识搜索中查有关rabies virus glycoprotein gene的内容
在数字搜索中查有关rabies virus glycoprotein gene的内容
在概念知识元中查有关rabies virus glycoprotein gene的内容
在学术趋势中查有关rabies virus glycoprotein gene的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社