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pci plasmid
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  “pci plasmid”译为未确定词的双语例句
     Methods: Both p40 and p35 cDNA of human interleukin 12 were amplified by means of reverse transcription and polymerase chain reaction (RT PCR) from total RNA of human bone marrow mixed nucleated cells. The amplified cDNA fragments were inserted into pCI plasmid repectively.
     方法 :采用RT PCR从上海地区人骨髓有核细胞中克隆IL 12的双亚基cDNA ,并插入质粒 pCI中进行全基因序列分析。
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     Methods The cell total RNA was extracted from human osteoblast cell,the BMP2 cDNA was amplified by RT PCR and then inserted into cloning vector pCI plasmid.
     方法 :由人成骨细胞中提取细胞总RNA ,利用逆转录PCR方法扩增获得人骨形成蛋白 2基因cDNA ;
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     Conclusion:The recombinant plasmid pCI-Ag85A has been constructed.
     coliJM1 0 9,筛选阳性克隆酶切鉴定。 结果 :经酶切鉴定 ,证实结核分枝杆菌重组真核表达质粒pCI-Ag85A构建正确。
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     the plasmid was transformed into E.
     coli JM 109扩增此质粒后经CpG甲基化酶M.
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     The recombinant plasmid of E.
     应用细菌质粒转化技术,将大肠杆菌K_(?)
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     The Construction of Plasmid pCI-S-IRES-ProT_α and Its in Vitro Expression
     质粒pCI-S-IRES-ProT_α的构建及其体外表达
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     PCI Bus Technology
     PCI总线技术
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  pci plasmid
No DHBV core gene products were detected in the control cells transfected with empty pCI plasmid.
      


Objective: To clone IL 12 p40 and p35 cDNA from Chinese and to construct bicistronic adenovirus vector containing human IL 12 p40 and p35 cDNA. Methods: Both p40 and p35 cDNA of human interleukin 12 were amplified by means of reverse transcription and polymerase chain reaction (RT PCR) from total RNA of human bone marrow mixed nucleated cells. The amplified cDNA fragments were inserted into pCI plasmid repectively. The total nucleotide sequences of both p40 and p35 cDNA were sequenced . Bicistronic...

Objective: To clone IL 12 p40 and p35 cDNA from Chinese and to construct bicistronic adenovirus vector containing human IL 12 p40 and p35 cDNA. Methods: Both p40 and p35 cDNA of human interleukin 12 were amplified by means of reverse transcription and polymerase chain reaction (RT PCR) from total RNA of human bone marrow mixed nucleated cells. The amplified cDNA fragments were inserted into pCI plasmid repectively. The total nucleotide sequences of both p40 and p35 cDNA were sequenced . Bicistronic adenovirus vectors (pAd p40 p35) which encode both human IL 12 subunits (p35 and p40) simultaneously were constructed by using p△ElsplA and internal ribosome entry site (IRES). The structure of pAd p 40 p35 is identified by means of restriction enzyme analysis . Results: IL 12 p40 and p35 cDNA sequences cloned from Shanghai bone marrow nucleated cells have similarities and differences when compared with Beijing p40, CLMF p40, CLMF p35, NKSF p40, NKSF p35. Restriction enzyme analysis shows that the struction of IL 12 bicistronic adenovirus vector was right. Conclusion: Our research data indicate that polymorphism might exist in the human p40 and p35 gene. The IL 12 bicistronic adenovirus vector might be applied to tumor immuno gene therapy.

目的 :克隆中国人IL 12的 p40和p35亚基cDNA ,构建含hIL 12双顺反子的腺病毒载体。 方法 :采用RT PCR从上海地区人骨髓有核细胞中克隆IL 12的双亚基cDNA ,并插入质粒 pCI中进行全基因序列分析。利用质粒 p△ElsplA和IRES序列构建同时表达p35和 p40亚基的腺病毒载体。酶切鉴定构建物中基因插入的正确性。结果 :上海地区人骨髓有核细胞中IL 12的 p40和p35cDNA基因同已报道的北京地区人DC细胞中IL 12的 p40和国外报道的NKSF ,CLMF的 p40和p35序列各有异同 ,构建的IL 12双亚基同时表达的腺病毒载体酶切鉴定正确。 结论 :人IL 12的 p40和 p35基因可能存在多态性 ,同时表达IL 12双亚基的腺病毒载体构建成功 ,对开展人IL 12基因治疗肿瘤具有重要意义。

Objective To clone the human recombined bone morphogenetic protein 2(BMP2) gene in Escherichia coli .Methods The cell total RNA was extracted from human osteoblast cell,the BMP2 cDNA was amplified by RT PCR and then inserted into cloning vector pCI plasmid.The recombined clone was confirmed by restriction enzyme and sequencing analysis.Result Restriction enzyme assay and sequencing showed that the constructed clone contained the BMP2 cDNA.Conclusion Cloning of recombined human BMP2 gene give...

Objective To clone the human recombined bone morphogenetic protein 2(BMP2) gene in Escherichia coli .Methods The cell total RNA was extracted from human osteoblast cell,the BMP2 cDNA was amplified by RT PCR and then inserted into cloning vector pCI plasmid.The recombined clone was confirmed by restriction enzyme and sequencing analysis.Result Restriction enzyme assay and sequencing showed that the constructed clone contained the BMP2 cDNA.Conclusion Cloning of recombined human BMP2 gene give a basis for further expression study.

目的 :在大肠杆菌中克隆人骨形成蛋白 2基因。方法 :由人成骨细胞中提取细胞总RNA ,利用逆转录PCR方法扩增获得人骨形成蛋白 2基因cDNA ;将获得基因片段重组到pCI质粒中 ,转化到大肠杆菌Top10后挑选克隆 ,利用限制性酶切和核苷酸序列分析鉴定重组质粒。结果 :质粒DNA酶切分析及核酸序列分析证实 ,获得的基因片段为人BMP2全编码序列。结论 :克隆获得人骨形成蛋白 2基因 ,为表达骨形成蛋白 2奠定了基础。

 
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