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ectopia osteogenesis
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  异位成骨
     3. To explore the appropriate mode of fabricating tissue engineered bone, the ectopia osteogenesis in vivo of the tissue engineered bone fabricated by osteoblast cultured with CHA was studied.
     (3)研究成骨细胞在CHA支架材料上复合培养后构建的组织工程化骨组织的体内异位成骨能力,探讨适宜的组织工程化骨组织的构建方式。
短句来源
     Conclusion: Collagen may be a suitable sustained release system for rhBMP-2 in animal experiment. And BMSCs may have important effect on enhancing ectopia osteogenesis.
     结论 :动物实验中胶原是rhBMP - 2适宜的缓释载体 ,BMSCs对促进材料异位成骨有重要意义
短句来源
     Osteoblasts were adhered to poly lactide co glycolide(PLGA) and then the compounds were implanted into the muscle of nude mice. The status of ectopia osteogenesis among compounds,PLGA and hOB were assayed by hard tissue section and toluidine blue staining.
     将成骨细胞复合PLGA植入裸鼠体内,硬组织切片,甲苯氨蓝染色,比较其与单纯植入成骨细胞或聚丙交酯-乙交酯(PLGA)时的异位成骨情况。
短句来源
     Conclusions The tissue engineered bone fabricated by osteoblasts which derived from hBMSCs cultured onto CHA have ablilities of ectopia osteogenesis in vivo. It is supposed to be a good way to repair clinical bone defect. [Key words] Tissue engineering;
     结论 成人骨髓源成骨细胞与CHA复合培养后构建的组织工程化骨组织 ,具有良好的异位成骨能力 ,可望应用于临床修复骨缺损。
短句来源
     Then, the polybutylcyanoacrylate nanoparticles were fabricated to be carrier of rhBMP-2, and experiments about biological effects of such nanoparticles on cultured BMSCs were done in order to clarify the efficiency of the nanoparticles on sustained releasing rhBMP-2. At last, experiments on ectopia osteogenesis and repair of rabbitcritical-sized cranial defect by tissue engineered bone fabricated by coral, BMSCs and sustainedly released rhBMP-2 by collagen were done.
     其次制备聚氰基丙烯酸正丁酯纳米微球作为rhBMP-2的载体,观察其对骨髓间充质干细胞的生物学效应,明确其对rhBMP-2的缓释效果。 最后构建以胶原为载体缓释rhBMP-2复合BMSCs及珊瑚的组织工程骨,观察其异位成骨及修复动物颅骨极限缺损的能力,以证实胶原的缓释效果以及胶原和BMSCs对促进成骨的重要意义。
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  “ectopia osteogenesis”译为未确定词的双语例句
     Inoculation of marrow derived osteoblasts to ectopia osteogenesis in coral granules
     骨髓来源成骨细胞接种于珊瑚颗粒中异位成骨的观察
短句来源
     Experiment on Ectopia Osteogenesis by Tissue Engineered Bone Fabricated by Coral, Bone Mesenchymal Stem Cells and Sustainedly Released rhBMP-2 by Collagen
     以胶原缓释rhBMP-2复合BMSCs及珊瑚构建组织工程骨异位成骨的实验研究
短句来源
     Study on ectopia osteogenesis of the composite of bone mesenchymal stem cells and bone matrix gelatin in vivo
     骨髓基质干细胞与骨基质明胶复合培养体内异位成骨的实验研究
短句来源
     The compounds of CHA with cells were implanted into nude mouse, and the ectopia osteogenesis was detected. In addition, Green fluorescent protein (GFP) can be used as a tracer on osteoblasts transfection by an adenovirus vector, which may provide reference for further research.
     此外,还采用绿色荧光蛋白(green fluorescent protein,GFP)基因转染骨髓源成骨细胞作为示踪标记,为进一步研究提供实验依据。
短句来源
     Objective: To evaluate ectopia osteogenesis by tissue engineered bone fabricated by coral, BMSCs and sustainedly released rhBMP-2 by collagen.
     目的 :观察以胶原缓释rhBMP - 2复合BMSCs及珊瑚构建的组织工程骨异位成骨的能力。
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  相似匹配句对
     Inoculation of marrow derived osteoblasts to ectopia osteogenesis in coral granules
     骨髓来源成骨细胞接种于珊瑚颗粒中异位成骨的观察
短句来源
     The Distraction Osteogenesis for Jawbone
     颌骨牵引成骨技术
短句来源
     3)Distraction osteogenesis,DO;
     3)牙槽骨牵引成骨术;
短句来源
     Study on ectopia osteogenesis of the composite of bone mesenchymal stem cells and bone matrix gelatin in vivo
     骨髓基质干细胞与骨基质明胶复合培养体内异位成骨的实验研究
短句来源
     INGUINAL ECTOPIA AND HERNIA OF OVARY
     卵巢的腹股沟异位与腹股沟疝
短句来源
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Objective To explore the appropriate mode of fabricating tissue engineered bone, the ectopia osteogenesised of the tissue engineered bone fabricated by osteoblasts which derived from adult human bone marrow stromal cells(hBMSCs) co cultured with coral hydroxyapatite(CHA) was observed. Methods Bone marrow was aspirated from the healthy adult human and culture expanded in vitro by the method of total marrow. hBMSCs were induced to differentiate...

Objective To explore the appropriate mode of fabricating tissue engineered bone, the ectopia osteogenesised of the tissue engineered bone fabricated by osteoblasts which derived from adult human bone marrow stromal cells(hBMSCs) co cultured with coral hydroxyapatite(CHA) was observed. Methods Bone marrow was aspirated from the healthy adult human and culture expanded in vitro by the method of total marrow. hBMSCs were induced to differentiate into osteoblasts. Then these differentiated osteoblasts were seeded onto CHA, co cultured for 5 days, the compounds with cells and CHA were implanted into musclar pouch of thigh of nude mouse and CHA alone was implanted as a control. The effectiveness of bone formation was assessed separately by means of gross observation, roentgenography, histology after implantation for 4,8,12 weeks. Human Alkaline phosphatase(ALP) and osteocalcin(OCN)of the compound were detected by RT PCR at the end of the 4th week. Results hBMSCs proliferated actively in primary and passage culture. These adult human osteoblasts derived from bone marrow could grow as well on CHA as normal cultured cells: New osteogenesis was detected at the end of the 4th, 8th and 12th week after implantation separately in experimental group. With time going by, the new bone formation increased. ALP and OCN derived from human by RT PCR were positively expressed, while no new bone formation was found in each of the control group. Conclusions The tissue engineered bone fabricated by osteoblasts which derived from hBMSCs cultured onto CHA have ablilities of ectopia osteogenesis in vivo. It is supposed to be a good way to repair clinical bone defect. [Key words] Tissue engineering; Bone marrow stromal cells; Cell culture; Osteoblast; Coral hydroxyapatite

目的 观察成人骨髓源成骨细胞与珊瑚羟基磷灰石 (CHA)复合构建的组织工程化骨组织的体内异位成骨能力 ,探讨适宜的组织工程化骨组织的构建方式。 方法 抽取健康成人骨髓 ,采用全骨髓法培养 ,使成人骨髓基质干细胞 (hBMSCs)定向诱导分化为成骨细胞 ,然后种植于CHA上 ,复合培养 5d后植入裸鼠股部肌袋内 ,以未种植细胞的CHA作为对照。术后 4,8,12周取材作一般观察、X线摄片、组织学检查和源于成人的碱性磷酸酶 (ALP)及骨钙素 (OCN)的RT PCR检测。 结果 原代和传代培养的细胞具有活跃的增殖能力 ,成骨细胞与CHA复合生长良好。实验组术后 4、8、12周均有新骨形成 ,随着时间延长 ,新骨生成量增多 ;对照组则均无新骨形成。术后 4周实验组RT PCR检测源于人的ALP及OCN表达均为阳性 ,对照组阴性。 结论 成人骨髓源成骨细胞与CHA复合培养后构建的组织工程化骨组织 ,具有良好的异位成骨能力 ,可望应用于临床修复骨缺损。

Objective: To evaluate ectopia osteogenesis by tissue engineered bone fabricated by coral, BMSCs and sustainedly released rhBMP-2 by collagen. Methods: Three scaffolds were fabricated: rhBMP-2/coral group, collagen/rhBMP-2/coral group and BMSCs/collagen/rhBMP-2/coral group. Osteogenesis was evaluated after implantation of scaffolds under skin of nude mice 8 weeks later. Results: It showed that osteogenesis in BMSCs/collagen/ rhBMP-2/coral group was more than that in collagen/rhBMP-2/coral group,...

Objective: To evaluate ectopia osteogenesis by tissue engineered bone fabricated by coral, BMSCs and sustainedly released rhBMP-2 by collagen. Methods: Three scaffolds were fabricated: rhBMP-2/coral group, collagen/rhBMP-2/coral group and BMSCs/collagen/rhBMP-2/coral group. Osteogenesis was evaluated after implantation of scaffolds under skin of nude mice 8 weeks later. Results: It showed that osteogenesis in BMSCs/collagen/ rhBMP-2/coral group was more than that in collagen/rhBMP-2/coral group, and osteogenesis in rhBMP-2/coral was least. Conclusion: Collagen may be a suitable sustained release system for rhBMP-2 in animal experiment. And BMSCs may have important effect on enhancing ectopia osteogenesis.

目的 :观察以胶原缓释rhBMP - 2复合BMSCs及珊瑚构建的组织工程骨异位成骨的能力。方法 :构建 3种复合支架材料 :1)rhBMP - 2 /珊瑚 ;2 )胶原 /rhBMP - 2 /珊瑚 ;3)BMSCs/胶原 /rhBMP - 2 /珊瑚。分别植入裸鼠皮下 ,8周后观察成骨情况 ,并作比较。结果 :第 3组材料异位成骨的能力最强 ,第 2组次之 ,第 1组较弱。结论 :动物实验中胶原是rhBMP - 2适宜的缓释载体 ,BMSCs对促进材料异位成骨有重要意义

AIM:To study the feasibility of orthopedic cell therapy by means of human bone marrow mesenchymal stem cells(hMSCs). METHODS:The experiment was done in the orthopaedic laboratory,the Third Hospital of Peking University from June 2002 to February 2003.hMSC was harvested from donors undergoing spinal surgery in the Third Hospital of Peking University without any metabolic disease detected before operation.hMSC was differentiated into osteoblasts directionally and amplified greatly by culturing in an osteo inductive...

AIM:To study the feasibility of orthopedic cell therapy by means of human bone marrow mesenchymal stem cells(hMSCs). METHODS:The experiment was done in the orthopaedic laboratory,the Third Hospital of Peking University from June 2002 to February 2003.hMSC was harvested from donors undergoing spinal surgery in the Third Hospital of Peking University without any metabolic disease detected before operation.hMSC was differentiated into osteoblasts directionally and amplified greatly by culturing in an osteo inductive conditioned medium.The difference of bone morphogenic protein 2 (BMP 2) mRNA expression in hMSC and osteoblasts was compared by reverse transcriptase polymerase chain reaction(RT PCR).Osteoblasts were adhered to poly lactide co glycolide(PLGA) and then the compounds were implanted into the muscle of nude mice.The status of ectopia osteogenesis among compounds,PLGA and hOB were assayed by hard tissue section and toluidine blue staining. RESULTS:The expression of BMP 2 mRNA in osteoblasts was obviously stronger than that of uninduced and undifferentiated hMSC.Four weeks after implantation, a little bone like tissue was found in the group of compounds of osteoblasts and PLGA,while mature lamellar bone was observed after 8 weeks,but there was no bone tissue in the PLGA group, and only a little bone like tissue was found in the osteoblasts group after 8 weeks. CONCLUSION:hMSC can be induced and differentiated into osteoblasts directionally and greatly amplified by culturing in an osteo inductive conditioned medium.After hMSC were induced into osteoblasts, the expression of BMP 2 mRNA is significantly raised.Mature lamellar bone will form after the compounds of osteoblasts and PLGA are implanted into the muscle of nude mice.hMSC has great application potential in treating bone defect and nonhealing of bone.

目的:探讨应用人骨髓基质干细胞(humanmarrowmesenchymalseemcell,hMSC)进行骨科细胞治疗的可行性。方法:实验于2002-06/2003-02在北京大学第三医院骨科实验室完成。hMSC取自北京大学第三医院骨科脊柱外科手术患者,术前经检测无代谢性疾病。hMSC在诱导培养基的作用下定向分化为成骨细胞并大量扩增。RT-PCR法比较骨形成蛋白(BMP)-2mRNA在hMSC和成骨细胞中的表达差异。将成骨细胞复合PLGA植入裸鼠体内,硬组织切片,甲苯氨蓝染色,比较其与单纯植入成骨细胞或聚丙交酯-乙交酯(PLGA)时的异位成骨情况。结果:BMP-2mRNA在成骨细胞中的表达明显强于未经诱导分化的hMSC;成骨细胞与PLGA复合植入4周可见少量成骨,8周后可见成熟板层骨形成,PLGA组未见成骨,成骨细胞组8周仅可见少量成骨。结论:hMSC在诱导培养基的作用下可定向分化为成骨细胞并大量扩增。分化为成骨细胞后,BMP-2mRNA的表达显著升高。成骨细胞与PLGA复合植入裸鼠体内,能形成成熟的板层骨。hMSC在治疗骨缺损、骨不愈合等方面具有巨大的应用潜力。

 
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