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acute irradiation injuries
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     AIM: To study the changes of cellular immune function in mice with moderate acute irradiation injuries and the protective effects of Yangyin Kangdu powder, a traditional Chinese medicine, on the injuries.
     目的 :研究小鼠急性中度骨髓型放射损伤细胞免疫功能的变化及养阴抗毒散的防护作用 .
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  相似匹配句对
     Treatment of acute laryngeal injuries
     喉外伤的急诊处理
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     acute injury is the main of injuries' character;
     损伤性质主要是急性损伤;
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     Paclitexel accentuates irradiation-induced pulmonary injuries
     紫杉醇加重放射性肺损伤的实验研究
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     Study on Effect of Microwave Irradiation in Patient with Acute Pancreatitis
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     Fetal liver cells transplantation in the treatment of extremely severe acute radiation injuries induced by large dose irradiation in leukemic patients
     胎肝细胞移植治疗白血病大剂量照射引起的极重度急性放射病
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In order to evaluate the changes in T cell subsets after acute irradiation injury, and investigate the mechanism of deficiency of cellular immunity in acute irradiation syndrome, Balb/c mice were exposed to a single total body irradiation of 5Gy gamma rays from a 60 Co source. On days 10, 28 and 120, the amounts of CD4 + and CD8 + subsets in spleen cells were evaluated by flow cytometry (FCM), and expression of IL 4 gene in ConA stimulated spleen cells was evaluated...

In order to evaluate the changes in T cell subsets after acute irradiation injury, and investigate the mechanism of deficiency of cellular immunity in acute irradiation syndrome, Balb/c mice were exposed to a single total body irradiation of 5Gy gamma rays from a 60 Co source. On days 10, 28 and 120, the amounts of CD4 + and CD8 + subsets in spleen cells were evaluated by flow cytometry (FCM), and expression of IL 4 gene in ConA stimulated spleen cells was evaluated by reverse transcription linked polymerase chain reaction, in which β-actin gene was used as an internal control. It was found that after irradiation, the ratio of CD4 + /CD8 + became significantly higher than that of the controls, and then the ratio lowered gradually until day 120. However, it still did not return to the level of the controls. On day 10, expression of IL 4 gene at mRNA level increased significantly. The results indicated that CD8 + subset was more radiosensitive than CD4 + , and the pattern of cytokine seeretion shifted to that of Th2 soon after irradiation.

为了解T细胞功能亚群在急性辐射损伤后的改变 ,探讨急性放射病细胞免疫功能长期低下的发病机制 ,用60 Coγ射线 5Gy对二级Balb/c小鼠一次性全身照射。在照射后约 10、2 8和 12 0天用双标记免疫荧光流式细胞术检测脾脏淋巴细胞CD4 + 和CD8+ T细胞亚群 ,用相对定量的RT PCR法检测脾脏细胞经ConA刺激活化后IL 4基因表达情况。结果发现 ,照射组相对于对照组CD4 + /CD8+ 比值显著升高 ,随着照射后时间的延长 ,CD4 + /CD8+ 比值逐渐降低 ,但至 12 0天左右仍未恢复到同批正常对照的水平。受照后 10天左右的小鼠脾细胞IL 4基因在mRNA水平明显升高。提示小鼠CD8+ T细胞比CD4 + T细胞辐射敏感性强 ,且照射后Th1/Th2的细胞因子分泌模式是向Th2型偏移的

AIM: To study the changes of cellular immune function in mice with moderate acute irradiation injuries and the protective effects of Yangyin Kangdu powder, a traditional Chinese medicine, on the injuries. METHODS: The mice models of 6 Gy X ray irradiation were established and the mice were randomized into control (A), irradiation (B) and irradiation plus Yangyin Kangdu powder (C) groups. T lymphocyte subpopulations of peripheral blood (including CD4 and CD8) were measured by direct...

AIM: To study the changes of cellular immune function in mice with moderate acute irradiation injuries and the protective effects of Yangyin Kangdu powder, a traditional Chinese medicine, on the injuries. METHODS: The mice models of 6 Gy X ray irradiation were established and the mice were randomized into control (A), irradiation (B) and irradiation plus Yangyin Kangdu powder (C) groups. T lymphocyte subpopulations of peripheral blood (including CD4 and CD8) were measured by direct microvolume whole blood immunoflurescent staining technique by flow cytometry, and erythrocyte immune functions (including RBC C 3bRR and RBC ICR) and WBC quantity were observed to evaluate the immune function of mice. RESULTS: The values of CD4, CD8 in groups B and C were significantly lower than those in control group ( P < 0.01) and the CD4/CD8 ratio in group B was significantly lower than that in control group ( P <0.01). The RBC C 3bRR ratio in group B significantly decreased on d 12, 20 and 28 after irradiation ( P <0.01) and the RBC ICR rosette ratio increased on d 4, 8 and subsequently declined to normal range. The RBC C 3bRR rosette ratio decreased only on the 12th d and the RBC ICR rosette ratio increased only on the 4th d in group C. The RBC C 3bRR rosette ratio in group C was higher than that in group B on d 4, 12, 20 and 28 ( P <0.01). CONCLUSION: The cellular immune function of the mice irradiated by 6 Gy X ray is low and Yangyin Kangdu powder has some protective effects on the cellular immune function after irradiation injuries.

目的 :研究小鼠急性中度骨髓型放射损伤细胞免疫功能的变化及养阴抗毒散的防护作用 .方法 :以 6GyX射线照射小鼠为动物模型 ,随机分为正常对照组 (A)、照射组 (B)及照射加中药组 (C) .用流式细胞仪直接免疫荧光染色法测定外周血T淋巴细胞亚群 ,酵母菌红细胞花环试验法测定红细胞免疫能力 .结果 :与A组比较 ,B ,C两组小鼠CD4 ,CD8百分率显著减少 (P <0 .0 1 ) ;B组CD4 /CD8比值明显降低 (P<0 .0 1 ) .B组小鼠RBC C3 bR花环率第 1 2 ,2 0 ,2 8日降低 (P<0 .0 1 ) ,RBC IC花环率第 4日和第 8日升高 (P <0 .0 1 ) ;C组小鼠RBC C3 bR花环率仅在第 1 2日下降 (P <0 .0 1 ) ,RBC IC花环率仅在第 4日升高 (P <0 .0 1 ) ;C组RBC C3 bR花环率在第 4 ,1 2 ,2 0 ,2 8日均高于B组 (P <0 .0 1 ) .结论 :6GyX线照射可造成小鼠细胞免疫功能低下 ,养阴抗毒散对此有一定的防治作用 .

 
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