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fat body cells
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  脂肪体细胞
     This study was focused on how to establish cell lines of insect fat bodies through primary culture and to explore the mechanism of proliferation of insect fat body cells in vitro, in order to provide reliable methods to establish more insect cell lines, in which the methods of explanted culture of animal tissues was refered.
     本研究参考动物细胞培养中植块培养的方法,从昆虫幼虫脂肪体细胞的原代培养入手,系统研究了以昆虫脂肪体组织为实验材料建立昆虫细胞系的方法,并初步探讨了昆虫细胞在体外增殖的机制,试图为更多昆虫脂肪体细胞系的建立提供有效的技术手段。
短句来源
     The juvenile hormone analogue ZR-515 stimulates development of the roughendoplasmic reticulum in fat body cells,the increase of chromatin clumps andpermeability of the nuclear envelop. JHA ZR-515 acts to cut down the rate ofreduction of the large vacuoles.
     保幼激素类似物(JHA)ZR-515能促进脂肪体细胞粗面内质网的发展,核中染色质块的增加以及核膜通透性的增强,对大型液泡的缩小有减缓作用。
短句来源
     At previtellogenic stage (that is, at pupal stage), large amounts of protein granules and mitochondria were present in fat body cells, but no rough endoplasmic reticulum (RER) could be observed.
     卵黄发生前(蛹期)的脂肪体细胞含有大量蛋白颗粒和线粒体,但未见粗面内质网,早期蛹脂肪体细胞中可见多囊泡体;
短句来源
     The protein synthesis in fat body cells displayed pulse like fluctuation.
     脂肪体细胞内蛋白质合成作用是现脉冲式的变化。
短句来源
     However, there were large amounts of RER as well as mitochondria in fat body cells, and large lipid droplets also occurred during vitellogenesis (that is, the period from pharate adult stage to 5 d after emergence).
     卵黄发生期(隐雌成蜂~5日龄雌成蜂)的脂肪体细胞内粗面内质网和线粒体均较为丰富.
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  “fat body cells”译为未确定词的双语例句
     POLYPLOIDY IN THE FAT BODY CELLS OF ADULT COCCINELLA SEPTEMPUNCTATA L.
     七星瓢虫成虫脂肪体细胞核的多倍性现象
短句来源
     PIERIS BRASSICAE GRANULOSIS VIRUS MORPHOGENESIS AND ULTRASTRUCTURAL CYTOPATHOLOGY OF INFECTED FAT BODY CELLS
     大菜粉蝶颗粒体病毒的形态发生及细胞病理的超微结构观察
短句来源
     Morphogenesis of Vanessa urticae Nuclear Polyhedrosis Virus in Infected Fat Body Cells
     荨麻蛱蝶核型多角体病毒在病虫脂肪体中的形态发生
短句来源
     The juvenile hormone analogue ZR515 was applied in adequate doses onto the body surface of the 5th instar larvae of the silkworm Bombyx mori L. It was observed that the activity of arginase in the fat body cells increased, the 5th stadium was prolonged, the weights of the cocoon and cocoon shell also increased.
     用不同剂量的JHAZR515处理家蚕,观察到在适当剂量时,精氨酸酶活力明显地高于对照组,提高范围从30%到110%;
短句来源
     The juvenile hormone analogue ZR515 was applied in adequate doses onto the bodysurface of the 5th instar larvae of the silkworm Bombyx mori L. It was observed thatthe activity of arginase in the fat body cells increased, the 5th stadium was prolonged,the weights of the cocoon and cocoon shell also increased.
     用不同剂量的JHAZR515处理家蚕,观察到在适当剂量时,精氨酸酶活力明显地高于对照组,提高范围从30%到110%;
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  fat body cells
Furthermore, pulse-labeling analysis showed that BmNPV infection restored protein synthesis in fat body cells.
      
The virus replicated in nuclei of fat body cells, and was transmitted per os to a small proportion of first instarG.
      
Hemocytes and fat body cells (FBC) could be detected as JEV antigen positive cells on day 1.
      
Transmission electron microscopy revealed the characteristic crystalline arrays of the hexagonal virus particles in the cytoplasm of fat body cells, tracheal matrix, muscle, hypodermis and blood cells.
      
In the former nymphs from mature galls, ovaries and mycetomes were degenerate and replaced by well-developed fat body cells.
      
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In the present paper it was shown that in the fat body cells of Bombyx mori L. we have been unable to demonstrate the activities of carbamyl phosphate synthetase, ornithine transcarbamylase and argininosuccinate synthetase, but arginase activity can be detected. It was suggested that the urea cycle may be absent. It is indicated from nutri tional studies that insects in general are unable to synthesize arginine for their needs, and we have also demonstrated in our other paper that the fat body cells...

In the present paper it was shown that in the fat body cells of Bombyx mori L. we have been unable to demonstrate the activities of carbamyl phosphate synthetase, ornithine transcarbamylase and argininosuccinate synthetase, but arginase activity can be detected. It was suggested that the urea cycle may be absent. It is indicated from nutri tional studies that insects in general are unable to synthesize arginine for their needs, and we have also demonstrated in our other paper that the fat body cells of Bombyx mori L. can form ornithine, urea, glutamate and proline at the expense of arginine and ketoglutarate. Thus, arginase may play an important role in the metabolism of amino acids in the silkworm. The activity of arginase was found to be the highest in the fat body of fifth instar larvae and adult silkworms and to be lowest in the embryo and pupae.The juvenile hormone analogue ZR515 was applied in adequate doses onto the body surface of the 5th instar larvae of the silkworm Bombyx mori L. It was observed that the activity of arginase in the fat body cells increased, the 5th stadium was prolonged, the weights of the cocoon and cocoon shell also increased.A major effect of juvenile hormone is to control sequential polymorphism, i. e. it is involved in the switching on and off of successive batches of genetic information. Its regulation role on enzyme activity may shed further light on the mechanism of action of the hormone.

尿素循环在生物体氮化合物代谢和调节控制中起着极为重要的作用。昆虫排出的尿素是否由于存在尿素循环而形成,尚未定论。我们对各种蚕体组织所作的研究表明,不存在尿素全循环,但脂肪体中精氨酸酶活力甚高,该酶与蚕的变态密切相关,并受保幼激素类似物(JHA)的调控。家蚕、蓖麻蚕和柞蚕脂肪体中,除精氨酸酶具有明显活力外,氨基甲酰磷酸合成酶,鸟氨酸转氨甲酰磷酸酶和精氨酸合成酶均无可检出的活力。在蚕幼虫期,尤其在五龄中后期,精氨酸酶活力最高;眠期和蛹期,该酶活力很低;蛹化蛾后,该酶活力又明显增高。用不同剂量的JHAZR515处理家蚕,观察到在适当剂量时,精氨酸酶活力明显地高于对照组,提高范围从30%到110%;当用高剂量ZR515处理蚕后,引起不结茧蚕,精氨酸酶活力先低于对照组,以后再逐步回升并高于对照组,而且持续增高的时间也较长。这表明蚕的变态和精氨酸酶活力的变化与JHA的量密切相关联并受JHA的调节控制。

After applying adequate doses of the juvenile hormone analogue ZR515 onto the surface of the 5th instar larvae of the silkworm Bombyx mori L. the activities of the transaminases of glyoine and alanine formation, suoh as L-alanine-glyoxylate transaminase, L-alanine-ketomalonate transaminase, L-aspartate-L-glutainate transaminase, and L-alanine-L-glutamate transaminase in the posterior division of the silkgland and the fat body cells were markedly increased as compared with the control. By using the juvenile...

After applying adequate doses of the juvenile hormone analogue ZR515 onto the surface of the 5th instar larvae of the silkworm Bombyx mori L. the activities of the transaminases of glyoine and alanine formation, suoh as L-alanine-glyoxylate transaminase, L-alanine-ketomalonate transaminase, L-aspartate-L-glutainate transaminase, and L-alanine-L-glutamate transaminase in the posterior division of the silkgland and the fat body cells were markedly increased as compared with the control. By using the juvenile hormone analogue it was observed that the 5th stadium was prolonged about one day and the weights of cocoon and cocoon shell were increased eaoh about 10%.From the above results it was shown that the increased synthesis of silk fibroin was not only the result of prolongation of the 5th stadium but also that of increased enzymatic activity under the influence of the juvenile hormone.The juvenile hormone analogue ZR777 was injected into Philosamia cynthia ricini B. The activities of the transaminases such as L-aspartate-L-glutamate transaminase, L-isoleucine-L-glutamate transaminase and L-alanine-glyoxalate transaminase were also inoreased.The mechanism of action of juvenile hormone in regulation and biosynthesis of silk fibroin was disoussed.

五龄后期蚕丝腺后部的细胞几乎只合成蚕丝的丝心蛋白。保幼激素类似物(JHA)能增加蚕丝心蛋白的生物合成。家蚕和蓖麻蚕丝心蛋白中,甘氨酸和丙氨酸的含量约占75%。我们用ZR515和ZR777分别处理五龄蚕后,观察到丝腺后部和脂肪体中合成甘氨酸和丙氨酸有关转氨酶的活力均有明显增加。(1)用ZR515处理五龄家蚕后,使五龄期延长1~2天,茧层量增加约10%。蚕丝腺后部的丙氨酸-乙醛酸转氨酶,丙氨酸-酮丙二酸转氨酶,谷丙转氨酶和谷天转氨酶活力,JHA组均比对照组增高约10~40%,脂肪体中这些酶活力也相应地比对照组增高约30%,其中丙氨酸-乙醛酸转氨酶活力增加到165%。酶活力增加的持续时间约3~5天。(2)用ZR777处理五龄蓖麻蚕后,丝腺后部和脂肪体中的谷天转氨酶、异亮氨酸吨-α-酮戊二酸转氨酶和丙氨酸-乙醛酸转氨酶活力,均比对照组高。本文认为JHA对蚕丝腺后部和脂肪体中形成甘氨酸和丙氨酸的转氨酶有明显的调控作用,从而为丝心蛋白的合成提供更多的甘氨酸和丙氨酸。这也许是JHA增丝机制的一个重要方面。

In the present paper it was shown that in the fat body cells of Bombyx mori L. wehave been unable to demonstrate the activities of carbamyl phosphate synthetase, orni-thine transcarbamylase and argininosuccinate synthetase, but arginase activity can bedetected. It was suggested that the urea cycle may be absent. It is indicated from nutri-tional studies that insects in general are unable to synthesize arginine for their needs,and we have also demonstrated in our other paper that the fat body cells...

In the present paper it was shown that in the fat body cells of Bombyx mori L. wehave been unable to demonstrate the activities of carbamyl phosphate synthetase, orni-thine transcarbamylase and argininosuccinate synthetase, but arginase activity can bedetected. It was suggested that the urea cycle may be absent. It is indicated from nutri-tional studies that insects in general are unable to synthesize arginine for their needs,and we have also demonstrated in our other paper that the fat body cells of Bombyxmori L. can form ornithine, urea, glutamate and proline at the expense of arginineand ketoglutarate. Thus, arginase may play an important role in the metabolism ofamino acids in the silkworm. The activity of arginase was found to be the highest inthe fat body of fifth instar larvae and adult silkworms and to be lowest in the embryoand pupae.The juvenile hormone analogue ZR515 was applied in adequate doses onto the bodysurface of the 5th instar larvae of the silkworm Bombyx mori L. It was observed thatthe activity of arginase in the fat body cells increased, the 5th stadium was prolonged,the weights of the cocoon and cocoon shell also increased.A major effect of juvenile hormone is to control sequential polymorphism, i. e. itis involved in the switching on and off of successive batches of genetic information.Its regulation role on enzyme activity may shed further light on the mechanism ofaction of the hormone.

尿素循环在生物体氮化合物代谢和调节控制中起着极为重要的作用。昆虫排出的尿素是否由于存在尿素循环而形成,尚未定论。我们对各种蚕体组织所作的研究表明,不存在尿素全循环,但脂肪体中精氨酸酶活力甚高,该酶与蚕的变态密切相关,并受保幼激素类似物(JHA)的调控。家蚕、蓖麻蚕和柞蚕脂肪体中,除精氨酸酶具有明显活力外,氨基甲酰磷酸合成酶,鸟氨酸转氨甲酰磷酸酶和精氨酸合成酶均无可检出的活力。在蚕幼虫期,尤其在五龄中后期,精氨酸酶活力最高;眠期和蛹期,该酶活力很低;蛹化蛾后,该酶活力又明显增高。用不同剂量的JHAZR515处理家蚕,观察到在适当剂量时,精氨酸酶活力明显地高于对照组,提高范围从30%到110%;当用高剂量ZR515处理蚕后,引起不结茧蚕,精氨酸酶活力先低于对照组,以后再逐步回升并高于对照组,而且持续增高的时间也较长。这表明蚕的变态和精氨酸酶活力的变化与JHA 的量密切相关联并受JHA 的调节控制。

 
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