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upstream regulation sequences
相关语句
  上游调控序列
     METHODS: The upstream regulation sequences of amelogenin gene were retrieved and analyzed.
     方法:检索并分析釉原蛋白基因的上游调控序列
短句来源
  “upstream regulation sequences”译为未确定词的双语例句
     Compared with the promoter of OsRacD cloned by reverse PCR from rice variety Nongken 58N, the homology was 99.8%, and the different nucleotides were outside the predicted response elements in promoter, suggesting that the fertility difference between Nongken 58S and Nongken 58N under long day conditions was not attributed to the difference in the structure of OsRacD upstream regulation sequences, but to the developmental regulation of gene differential expression.
     与反向PCR克隆的水稻农垦58N该基因的启动区比较,证实该基因的启动区在农垦58S和农垦58N的同源性达到99.8%,且在预测的调控元件上不存在碱基差异。 说明农垦58S和农垦58N在长日照条件下表现出的育性差别,并非两者在OsRacD调控序列结构上的差别所致,而与基因表达的发育调控有关。
短句来源
     METHODS: The upstream regulation sequences of amelogenin gene were amplified by PCR from mouse C57BL/6J DNA. Different transcriptional regulation sequences including the basal promoter were ligated with luciferase gene in PGL3-Basie vector. These report vectors were transient transfected into CHO, Hela and UMR-106 cells to analyse the transcription activation of these promoters.
     方法:PCR法从小鼠C57BL/6J基因组扩增不同长度的包括釉原蛋白基本启动子的转录调控序列,连入PGL3-Basic载体,瞬时转染CHO,Hela,UMR-106细胞,分析不同长度的启动子片段在 3种细胞中的活性。
  相似匹配句对
     Study on the engineering of channel regulation in upstream Hanjiang River
     韩江上游(梅江、汀江)航道整治工程试验研究
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     REGULATION OF HBV GENE EXPRESSION BY CORE PROMOTER AND ITS UPSTREAM SEQUENCE
     乙型肝炎病毒核心启动子及其上游顺序对其基因表达的调控
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     Regulation and Control
     约束与控制
短句来源
     On Legal Regulation
     论法律调整
短句来源
     METHODS: The upstream regulation sequences of amelogenin gene were retrieved and analyzed.
     方法:检索并分析釉原蛋白基因的上游调控序列。
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Genes encoding high molecular weight(HMW)glutenin, a wheat seed storage protein, are expressed only in the developing endosperm. It was previously subclones of a wheat ( Triticum aestivum L .cv. asarce) 2.8kb DNA that contained 0.4kb of HMW glutenin gene sequence and 2.4 kb of upstream regulation sequence. With the site specfic mutagensis method,a novel restriction enzyme site BamHⅠ is obtained by changing the three bases between TATA box and initiation codon ATG, and a series of truncated...

Genes encoding high molecular weight(HMW)glutenin, a wheat seed storage protein, are expressed only in the developing endosperm. It was previously subclones of a wheat ( Triticum aestivum L .cv. asarce) 2.8kb DNA that contained 0.4kb of HMW glutenin gene sequence and 2.4 kb of upstream regulation sequence. With the site specfic mutagensis method,a novel restriction enzyme site BamHⅠ is obtained by changing the three bases between TATA box and initiation codon ATG, and a series of truncated HMW glutenin gene promoters which are respectly 2400, 610, 440 and 110bp DNA fragments. Four fragments are inserted into plasmid pBI101.1 respectively and four plasmids of pWG2400, pWG600, pWG440 and pWG100 are constructed. The plasmid pWG2400 is transferred into the endosperm cells of Zea mays by means of particle gun,laser microbean and PEG, and the GUS gene is expressed in transgenic cells. These plasmids provide valuable materials for further analysis of HMW glutenin gene expression and regulation.

通过定点突变,在小麦高分子量(HMW)谷蛋白基因5′上游调控序列的TATAbox与基因翻译起始密码子ATG之间,改变三个碱基,产生新的BamHⅠ内切酶位点.在调控序列中,选择四个合适的内切酶,对上游序列进行缺失,分离到四个大小分别为2400、610、440和110bp的含有不同调控元件的HMW谷蛋白基因启动子,与质粒pBI101.1的报告基因GUS重组,构建了四个嵌合质粒pWG2400、pWG600、pWG440和pWG100.经基因枪、微束激光和PEG等方法将pWG2400转化玉米胚乳悬浮细胞,GUS基因获得表达.这为进一步研究HMW谷蛋白基因的调控机理奠定了基础.

Objective To appraise the relationship between fluconazole resistance and expression of the CDR1 gene upstream regulation sequence. Methods The DNA of fluconazole-susceptible and fluconazole-resistant Candida albicans isolates were extracted, PCRs were based on the special primer of upstream regulation sequence of Candida albicans CDR1 gene, and the mutation of the sequence was compared by DNA sequencing. Results No mutation was found in the upstream regulation sequence of Candida albicans...

Objective To appraise the relationship between fluconazole resistance and expression of the CDR1 gene upstream regulation sequence. Methods The DNA of fluconazole-susceptible and fluconazole-resistant Candida albicans isolates were extracted, PCRs were based on the special primer of upstream regulation sequence of Candida albicans CDR1 gene, and the mutation of the sequence was compared by DNA sequencing. Results No mutation was found in the upstream regulation sequence of Candida albicans CDR1 gene. Conclusions Candida albicans fluconazole resistance is not due to mutation of the upstream regulation sequence of Candida albicans CDR1 gene.

目的探讨白念珠菌耐药基因CDR1基因上游调控序列对氟康唑耐药的影响。方法分别抽提氟康唑耐药/敏感配对菌株DNA,通过特异性合成CDR1基因上游调控序列引物,分析该序列在氟康唑耐药/敏感配对菌株中是否存在基因突变。结果白念珠菌氟康唑耐药/敏感配对菌株中的CDR1基因上游调控序列未出现任何碱基突变和缺失。结论白念珠菌氟康唑耐药与CDR1基因上游调控序列是否突变无关。

PURPOSE: To clone different promoter region of amelogenin gene and analyze their transcriptional activity. METHODS: The upstream regulation sequences of amelogenin gene were retrieved and analyzed. Amplified by PCR from the genomic DNA of mouse C57BL/6J and digested with restriction endonucleases enzyme, different transcriptional regulation sequences of 5' flanking of amelogenin gene including the basal promoter were cloned and ligated with luciferase gene in PGL3- Basic vector. These report...

PURPOSE: To clone different promoter region of amelogenin gene and analyze their transcriptional activity. METHODS: The upstream regulation sequences of amelogenin gene were retrieved and analyzed. Amplified by PCR from the genomic DNA of mouse C57BL/6J and digested with restriction endonucleases enzyme, different transcriptional regulation sequences of 5' flanking of amelogenin gene including the basal promoter were cloned and ligated with luciferase gene in PGL3- Basic vector. These report vectors were transiently transfected into CHO, Hela and UMR- 106 cells and luciferase assay was performed to analyse the transcription activation of these promoters. RESULTS: 6 promoters different in length were cloned. The activity of luciferase was very strong in Hela cells. On the contrary, the CHO and UMR- 106 cells showed weak fluorescence. Luciferase activity fluctuated with the different promoter lengths in Hela cell as well as in CHO and UMR- 106 cells. 975 bp and 532 bp of amelogenin 5' flanking DNA had a strong transcriptional activation, but 285 bp of amelogenin 5 ' flanking DNA had a weaker transcriptional activation. CONCLUSION: The sequence of amelogenin promoter can be activated in Hela cell. Hela cell can be used as a good model to study the transcriptional regulation of amelogenin promoter. According to the different activities of different lengths, it is suggested that there were some potential siliencer located between - 975 and - 532, and some potential activator in the region between - 532 and - 285. Supported by National Natural Science Foundation of China (30271418).

目的:克隆釉原蛋白基因启动子及可能影响转录调控的序列,分析其在不同细胞中的转录模式。方法:检索并分析釉原蛋白基因的上游调控序列。利用PCR及酶切方法,从小鼠C57BL/6J基因组上扩增不同长度(包括基本启动子区域)的转录调控序列,与PGL3-Basic载体的虫荧光素酶基因连接。瞬时转染CHO、Hela、UMR-106细胞,检测荧光素酶的活性,分析不同长度的启动子片段在各种细胞中的转录活性。结果:共构建了6个不同长度的报告载体,瞬转后发现在Hela细胞中有较强的荧光素酶活性,在CHO、UMR-106细胞中活性很弱。在不同的细胞内,启动子活性随片段长度变化,其变化趋势有明显的相似性。表现为在转录起始点上游975bp与532bp的区域具有较强的转录活性,而转录起始点上游285bp区域的转录活性有所降低。结论:釉原蛋白的启动子可在Hela细胞中激活,Hela细胞可作为研究釉原蛋白启动子转录调控的细胞模型;初步判断釉原蛋白启动子上的-1693与-975之间的序列为转录抑制区域,-532与-285之间为转录增强区域。

 
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