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glycoprotein b gene
相关语句
  gb基因
     High-efficient Expression of Truncated Glycoprotein B Gene of Infectious Laryngotracheitis Virus and the Development of the tgB-ELISA
     传染性喉气管炎病毒gB基因片段的高效表达及tgB-ELISA方法的建立
短句来源
     In order to determine the optimum ratio of components in lyophilized bivalent vaccine against Marek′s disease(MD),vaccine preparations composed of recombinant fowlpox virus expressing glycoprotein B gene from CVI988/Rispens strain of MDV(rFPV-gB/R) and herpesvirus of turkey(HVT) were evaluated for their protective efficacy against very virulent MDV strains Md5 and RB1B in different chickens.
     对高效表达鸡马立克氏病(MD)血清型疫苗毒株CVI998/Rispens gB基因的重组鸡痘病毒(rFPV-gB/R)和火鸡疱疹病毒(HVT)组成MD二价冻干疫苗时2种病毒的最适剂量配比进行了研究。
短句来源
     PREPARATION AND SPECIFICITY OF INFECTIOUS LARYNGOTRACHEITIS VIRUS GLYCOPROTEIN B GENE PROBE
     鸡传染性喉气管炎病毒gB基因核酸探针的研究
短句来源
     High-efficient expression of the immune-dominant region of glycoprotein B gene of infectious laryngotracheitis virus
     传染性喉气管炎病毒gB基因的主要免疫原片段在大肠杆菌中的表达
短句来源
     Construction of Recombinant Fowlpox Virus Expressing Glycoprotein B Gene of Infectious Laryngotracheitis Virus and Its Protective Efficacy
     表达鸡传染性喉气管炎强毒gB基因的重组鸡痘病毒的构建及其免疫效力
短句来源
  糖蛋白b基因
     CONSTRUCTION AND IDENTIFICATION OF RECOMBINANT PLASMID CONTAINING THE GLYCOPROTEIN B GENE OF MAREKS'DISEASE VIRUS
     含有马立克氏病病毒糖蛋白B基因重组质粒的构建及其鉴定
短句来源
     MODIFICATION AND IDENTIFICATION OF RECOMBINANT PLASMID CONTAINING THE GLYCOPROTEIN B GENE OF MAREK'S DISEASE VIRUS
     含有马立克氏病病毒糖蛋白B基因重组质粒的改造及其鉴定
短句来源
     EXPRESSING MAREK'S DISEASE VIRUS GLYCOPROTEIN B GENE IN SILKWORM
     禽马立克氏病毒糖蛋白B基因在家蚕中的表达
短句来源
     PROTECTION STUDIES OF A RECOMBINANT FOWLPOX VIRUS VACCINE EXPRESSING GLYCOPROTEIN B GENE FROM CVI988/RISPENS STRAIN OF MAREK’S DISEASE VIRUS
     表达马立克氏病病毒CVI988/Rispens株糖蛋白B基因的重组鸡痘病毒疫苗的免疫保护研究
短句来源
     Genetic Stability and Protective Efficacy of the Recombinant Fowlpox Virus with Highly Expressed Glycoprotein B Gene from Serotype 1 Marek's Disease Virus
     表达鸡马立克氏病病毒糖蛋白B基因重组鸡痘病毒的遗传稳定性及免疫效力试验
短句来源
  “glycoprotein b gene”译为未确定词的双语例句
     Immunity and Safety of a Recombinant Fowlpox Virus Expressing Glycoprotein B Gene from CVI988/Rispens Strain of Marek′s Disease Virus
     表达马立克氏病病毒CVI988/Rispens糖蛋白B重组鸡痘病毒的免疫性及安全性
短句来源
     CLONING OF GLYCOPROTEIN B GENE FROM STRAIN RISPENS OF MAREK'S DISEASE VIRUS AND CONSTRUCTION OF RECOMBINANT FOWLPOX VIRUS
     马立克氏病病毒Rispens株B抗原基因的克隆及其重组鸡痘病毒的构建
短句来源
     CLONING AND SEQUENCING OF GLYCOPROTEIN B GENE FROM RISPENS STRAIN OF MAREK′S DISEASE VIRUS
     MDV Rispens 株B抗原基因的酶切及序列分析
短句来源
     Glycoprotein B gene(gB) of virulent MDV GA strain expressed in E.
     用大肠杆菌 BL2 1表达了马立克氏病病毒 (MDV)强毒 GA株的囊膜糖蛋白 B(g B)基因 ,通过 SDS- PAGE电泳分离表达蛋白条带 ,切下并碾碎后作为免疫原制备单克隆抗体。
短句来源
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  glycoprotein b gene
The glycoprotein B gene homologue from an olive baboon CMV isolate (BaCMV OCOM4-37) was identified, cloned and sequenced.
      
Characterization of the Glycoprotein B Gene from Ruminant Alphaherpesviruses
      
Identification and Sequence Analysis of the Glycoprotein B Gene of Porcine Cytomegalovirus
      
Herpes Simplex Virus 1 (HSV-1) Strain HSZP Glycoprotein B Gene: Comparison of Mutations among Strains Differing in Virulence
      
Identification of the murine cytomegalovirus glycoprotein B gene and its expression by recombinant vaccinia virus.
      


A rapid method for detection and identification of equine herpesvirus 1 (EHV-1) and equine herpesvirus 4 (EHV-4) was developed using polymerase chain reaction (PCR). Primers for PCR were designed based on nucleotide sequences of glycoprotein B genes of EHV 1 and EHV 4 to amplify specific regions for EHV 1 or EHV 4 or a common region of both viruses. By using type specific primers, amplified fragments were identified as EHV 1 or EHV 4 in a one step reaction. We have applied this technique to specimens...

A rapid method for detection and identification of equine herpesvirus 1 (EHV-1) and equine herpesvirus 4 (EHV-4) was developed using polymerase chain reaction (PCR). Primers for PCR were designed based on nucleotide sequences of glycoprotein B genes of EHV 1 and EHV 4 to amplify specific regions for EHV 1 or EHV 4 or a common region of both viruses. By using type specific primers, amplified fragments were identified as EHV 1 or EHV 4 in a one step reaction. We have applied this technique to specimens from abortive fetuses of Przewalskii horse in Xinjiang. The results indicate that PCR can be used for rapid, sensitive and specific detection and identification of EHV 1 and EHV 4. Furthermore, EHV 1 and EHV 4 can be clearly distiguished in a one step PCR reaction. It provides a new apporach for the diagnosis of the two virus types in future.

聚合酶链反应 (PCR)可作为一种快速检测并鉴别马疱疹病毒 1型 (EHV 1)和马疱疹病毒 4型 (EHV 4)的诊断方法。PCR引物是根据编码EHV 1和EHV 4的糖蛋白B(gB)基因上的共有的核苷酸序列或型特有的核苷酸序列设计的。利用这两种病毒的型特异性混合引物 ,在一步PCR反应中检测并鉴别EHV 1和EHV 4,而同一疱疹病毒科的单纯疱疹病毒 1型 (HSV 1)、伪狂犬病毒 (PRV)、马立克氏病病毒 (MDV)均无特异性扩增。应用建立的PCR方法检测了普氏野马流产胎儿病料 ,实验结果表明 ,这种PCR方法是一种直接检测并鉴定EHV 1和EHV 4的快速、敏感的诊断方法 ,同时 ,它可在一步PCR反应中直接鉴别这两种病毒 ,可用于病料中EHV 1和EHV 4检测的初步筛选。

Glycoprotein B gene (gB) of virulent MDV GA strain expressed in E.coli was cut off from SDS PAGE ,then inoculated it to BALB/C mice directly to prepare monoclonal antibody. 1 strain of monoclonal hybridoma cells 7C8 was obtained by ELISA and Indirect Fluorescence Assay(IFA). Its ascites fluids titre of ELISA and IFA is 1: 212 and 1:800 respectively. The Mab can also identify CEF infected by MDV through dot ELISA.

用大肠杆菌 BL2 1表达了马立克氏病病毒 ( MDV)强毒 GA株的囊膜糖蛋白 B( g B)基因 ,通过 SDS-聚丙烯酰胺凝胶电泳( SDS- PAGE)分离表达蛋白条带 ,切下并碾碎后作为免疫原制备单克隆抗体。通过酶联免疫吸附试验 ( ELISA)和间接免疫荧光试验( IFA) ,得到 1株 GA株阳性的单克隆抗体杂交瘤细胞株 7C8。该单抗腹水的 ELISA效价为 1∶ 2 1 2 ,IFA效价为 1∶ 80 0 ,与 MDV不同致病型毒株 CVI988、GA、RB1 B、MD1 1、6 48A株感染的鸡胚成纤维细胞 ( CEF)均呈IFA阳性。 1∶ 1 0 0稀释时与 GA感染的 CEF在斑点酶联免疫吸附试验 ( dot- EL ISA)中呈阳性。

Glycoprotein B gene(gB) of virulent MDV GA strain expressed in E.coli was cut off from SDS-PAGE,then inoculated it to BALB/c mice directly to prepare monoclonal antibody.1 strain of monoclonal hybridoma cells 7C8 was obtained by ELISA and Indirect Fluorescence Assay(IFA).Its ascites fluids titre of ELISA and IFA is 1∶2 12 and 1∶800 respectively.The McAb can also identify CEF infected by MDV through dot-ELISA.

用大肠杆菌 BL2 1表达了马立克氏病病毒 (MDV)强毒 GA株的囊膜糖蛋白 B(g B)基因 ,通过 SDS- PAGE电泳分离表达蛋白条带 ,切下并碾碎后作为免疫原制备单克隆抗体。通过 EL ISA和间接免疫荧光试验 (IFA) ,得到 1株阳性单克隆抗体杂交瘤细胞株 7C8。该单抗腹水的 EL ISA效价为 1∶ 2 1 2 ,IFA效价为 1∶ 80 0 ,与 MDV不同致病型毒株CVI988、GA、RB1B、MD11、6 48A株感染的鸡胚成纤维细胞 (CEF)均呈 IFA阳性。1∶ 10 0稀释时与 GA株感染的 CEF在斑点酶联免疫吸附试验 (dot- EL ISA)中呈阳性。

 
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