助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   mutant population 的翻译结果: 查询用时:0.181秒
图标索引 在分类学科中查询
所有学科
农作物
植物保护
农业基础科学
更多类别查询

图标索引 历史查询
 

mutant population
相关语句
  突变体库
     Establishing the T-DNA Insertion Mutant Population of Magnaporthe Grisea and Analyzing the Mutants
     稻瘟菌(Magnaporthe grisea)T-DNA插入突变体库的构建及其分析
短句来源
     Chlorophyll Contents and Net Photosynthetic Rates of T-DNA Inserted Rice Mutant Population
     T-DNA插入水稻突变体库的叶绿素和净光合速率变化
短句来源
     The Screening of Mutants and Construction of Mutant Population for Cultivar "9311" in Rice(Oryza sativa L.)
     水稻“9311”突变体筛选和突变体库构建
短句来源
     Optimization of Agrobacterium-mediated Transformation System and Preliminary Establishment of T-DNA Insertion Mutant Population in Rice
     农杆菌介导水稻转化系统的优化及水稻T-DNA插入突变体库的初步建立
短句来源
     Additionally, different rice mutants were obtained during generation of rice mutant population, such as partial-sterility plant, most -sterility plant, complete sterile plant and so on.
     此外,在水稻突变体库建立的过程中,还得到了各种不同的突变体,如不同程度的不育突变体、穗形和粒形发生变化的突变体等,这说明了我们的T-DNA作为插入标签是可以有效地诱导水稻基因产生突变的。
短句来源
更多       
  突变群体
     Generation of a large and saturated T-DNA insertion mutant population is an important and effective tool in rice functional genomic research.
     利用水稻T-DNA插入突变库,通过正向和反向遗传学的方法来研究基因的功能是现在功能基因组研究的有效方法,但首先是建立一个足够大、尽可能饱和的T-DNA突变群体
短句来源
     Rice Mutant Population and its Applications on Functional Genomics
     水稻突变群体的构建及功能基因组学
短句来源
     The major results obtained are as follows:1. An insertional mutant population of Nipponbare (Oryza sativa L. ssp. japonica) was developed using a novel and efficient Ac/Ds tagging system. We verified reliability and feasibility of Basta selection in the field. The selected transposants by spraying Basta in the fields were in agreement with the results of PCR detection in the laboratory, which showed that this system was suitable to construct rice large-scale insertional mutagenesis library.
     1.利用新型的Ac/Ds标签系统构建了水稻大规模插入突变群体,验证了新型Ds载体中BAR基因在田间大规模选择转座系的可靠性,可行性及有效性,结果发现Basta田间选择与实验室鉴定结果高度符合,表明该系统适合于水稻大规模插入突变体库的构建。
短句来源
     We treated a wheat doubled haploid line Yi4212 with this techniqu e by nitrogen ion and established a mutant population with 60 lines in our lab.
     据此利用该方法处理“遗4212”,建立起具有60个株系的突变群体
短句来源
  “mutant population”译为未确定词的双语例句
     45 nucleotide sequences of the clones derived from CHN9230-F3-Ⅱ could be divided into 3 groups in the phylogenetic tree base on VP1 gene homology,forming a viral mutant population with a few preponderant sequences,and this was the so called typical quasispecies.
     45条序列在同源进化树图上分为3组,但都来源于CHN9230-F3-Ⅱ株,组成以优势株为主的相关突变株的病毒群,是典型的居群样存在形式。
短句来源
     To confirm gene function by mutant analysis based on a saturated mutant population is a straight and efficient way.
     功能基因组学研究最直接最有效的方法是构建饱和的基因突变群体,通过突变体分析鉴定基因功能。
短句来源
     6 materials (including 5 mutants and 1 CK) were chosen from the mutant population, induced by EMS, to determine the content of crude protein, fat, soluble sugar and lysine. as well as the activity of peroxidase (POD) and superoxide dismutase (SOD) .
     本研究从经EMS诱变并已多代自交的玉米诱变群体中选取6个材料(包括1个对照),系统测定了各个材料的粗蛋白、粗脂肪含量以及可溶性糖含量和Lys含量,还测定了POD与SOD这2个酶的活性;
短句来源
     Gama-glutamylcysteine synthetase (GCS) is a rate-limiting enzyme in GSH biosynthesis. The GCS gene has been cloned in Arabidopsis thaliana and other plants, but has still not been reported in rice. From rice mutant population generated from T-DNA insertion, we cloned the rice GCS gene from mutant L395 by T-DNA tag cloning method, and named it OsGCS (Genbank accession No. AJ508915).
     利用该实验室T-DNA标签的编号为L395的水稻突变体,克隆了一个编码水稻谷胱苷肽(GSH)合成途径中关键酶即谷氨酰半胱氨酸合成酶(GCS)的基因,将其命名为OsGCS(Genbank accession No.AJ508915)。
短句来源
     Four inbred lines and 5 materials were chosen from the mutant population induced by EMS,and their 20 hybrids,were used to investigate the heterosis of 3 maize quality characters,including oil content(OC),protein content(PC),starch content(SC).
     以4个常用自交系作母本,5个经过EMS化学诱变处理并经多代自交纯合的材料作父本,按NCII设计配成20个杂交种,研究了其中3个品质性状的杂种优势表现。
短句来源
更多       
查询“mutant population”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  mutant population
By a T-DNA insertional mutagenesis approach, we have generated a rice mutant population containing 55,000 promoter trap and gene activation or knockout lines.
      
A rice phenomics study-phenotype scoring and seed propagation of a T-DNA insertion-induced rice mutant population
      
To identify salt tolerance determinants, we screened for double mutants from a T-DNA tagged sos3-1 mutant population in the Arabidopsis Col-0 gl1 background.
      
A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of ric
      
Our mutant population offers a highly valuable resource for high throughput rice functional analyses using both forward and reverse genetic approaches.
      
更多          


A wild type and thyee transposon Tn5 insertion mutants of Rhizobium fredii were used to study their survival and Tns phenotypic expression in soil under optimum temperature (28 C)and temperature stress (40 C). Survival studies with sterile and nonsterile soils show that under optimum temperature (28 C), biotic factors inhibit the growth of mutant and wild type. But, there is no significant difference (P=0. 01) between the surviving populations of wild type and Tn5 mutants. Under temperature...

A wild type and thyee transposon Tn5 insertion mutants of Rhizobium fredii were used to study their survival and Tns phenotypic expression in soil under optimum temperature (28 C)and temperature stress (40 C). Survival studies with sterile and nonsterile soils show that under optimum temperature (28 C), biotic factors inhibit the growth of mutant and wild type. But, there is no significant difference (P=0. 01) between the surviving populations of wild type and Tn5 mutants. Under temperature stressed condition (40 C), both wild type and mutant populations decline rapidly, and some of the mutant cells (ON-2 and ON-3) can't express kanamycin resistance. These results suggest that high temperature is a harmful factor for the survival of bacteria and the phenotypic expression of Tn5 in soil.

研究了3株弗氏中华根瘤菌(Rhizobiumfredii)Tns突变株于适宜温度和高温胁迫两种条件下在土壤中的存活和Tns表型的表达.在适宜温度(28℃)条件下的灭菌和未灭菌土壤中的存活研究表明生物因素抑制了突变株和野生型的生长.但野生型和突变株的存活种群密度之间无显著差异(P=0.01).在高温胁迫(40℃)条件下,土壤中野生型和突变株的种群密度迅速下降,其中部分ON-2和ON-3细胞丢失了Tns表型,说明部分细菌的Tn5表型在高温胁迫条件下不能表达.

With the completion of the rice genome sequence to assign a function to unknown or predicted gene becomes a major task in functional genomics. Functional genomics is a general approach toward understanding how the genes of an organism work together by assigning new functions to unknown genes. Now so many new technologies have been developed to exploit gene functions. Among them is to identify all genes by mutant analysis based on a saturated mutant population, which is a straight and efficient way to understand...

With the completion of the rice genome sequence to assign a function to unknown or predicted gene becomes a major task in functional genomics. Functional genomics is a general approach toward understanding how the genes of an organism work together by assigning new functions to unknown genes. Now so many new technologies have been developed to exploit gene functions. Among them is to identify all genes by mutant analysis based on a saturated mutant population, which is a straight and efficient way to understand the roles of all genes. In this paper, various methods to produce mutants and their applications on functional genomics were reviewed and commented. Spontaneous mutants can be used only as a supplemental resource because of its low frequency of mutation and its difficulty in identifying mutated genes. Some chemical mutagenesis such as EMS can be efficiently used to produce a large number of point mutants in a short period and the induced mutants can be detected by TILLING. But it is difficult to detect a point mutation and its phenotype when there are multiple point mutations in one mutant. Similarly, Fast neutron can be applied for mutagenesis and producing a large number of deletion-based mutants in a short period, and the deletion mutants can be screened by Deleteagene. However, it is also very difficult to identify a deletion mutant and its phenotype when the induced deletion occurs covering multiple genes or within an intron. Insertional mutagenesis based on T-DNAs, transposons and retrotransposons is becoming a major approach to produce a saturated mutant pool. A large number of T-DNA insertion lines have been produced in rice; but T-DNA insertional mutagenesis can be used only for those rice varieties with highly efficient transformation and it is difficult to analyze an insertion line because of complex patterns of T-DNA integration into the rice genome and mixture with mutations induced from tissue culture. The retrotransposon Tos17 has been successfully applied for rice functional genomics. But it is also difficult to identify a mutant related to Tos17 because there are multiple copies of Tos17 in a mutant and only about 10 percent of mutants are from Tos17 insertion. Theoretically, Ac/DS two-element system is regarded as a best approach for rice insertional mutagenesis. It is very easy to identify a mutant because of single copy of Ds insertion. An additional advantage is that Ds can be excised from disrupted gene in the presence of Ac transposase, resulting in phenotypic reversion to the wild type or giving rise to alleles with weaker phenotypes. However, it is also difficult to identify a mutant when there are Ds excision footprints in the mutant caused by Ds multiple excision-insertion events in the presence of Ac transposase. RNAi can efficiently silence a gene, but not all genes can be silenced. In addition, RNAi can interfere in genes with redundant and overlapping functions or gene families with high homolog in sequence, making it difficult identify a silenced gene. So it is obvious that each method has its advantage and disadvantage and different methods should be combined to produce a saturated mutant population.

随着水稻基因组全序列的测定完成 ,功能基因组学已成为重点研究内容。功能基因组学主要研究生物有机体内各基因的生物学功能进而了解所有基因如何协调发挥作用完成一系列的生长发育过程。目前已经发展了多种分析鉴定基因功能的方法 ,其中最直接最有效的方法是构建饱和的基因突变群体 ,通过突变体分析鉴定基因功能。本文主要阐述了各种构建方法及其优缺点以及在功能基因分离鉴定上的应用。自发突变的频率极低 ,且自发突变基因的分离难度比较大 ,只能作为突变群体构建的辅助方法。利用EMS等化学诱变剂可以在短时间内构建大量点突变群体 ,并可用TILLING进行突变检测 ,但多位点的点突变使突变表型难以鉴定。由快中子等物理诱变也可以在短时间内构建大量缺失突变体 ,且可用Deleteagene系统进行检测 ;但多基因缺失、多位点缺失和内含子缺失等使突变表型的分析可能无法进行。利用T -DNA、转座子和反转录转座子等构建插入突变体已经成为突变库构建的主要方法。T -DNA插入已成功应用于水稻大规模突变体的构建 ,但只限于转基因效率较高的品种 ;T -DNA在基因组中整合的复杂性以及转基因过程中由组织培养等引发的突变等 ,增加了突变体表型和分子分析...

随着水稻基因组全序列的测定完成 ,功能基因组学已成为重点研究内容。功能基因组学主要研究生物有机体内各基因的生物学功能进而了解所有基因如何协调发挥作用完成一系列的生长发育过程。目前已经发展了多种分析鉴定基因功能的方法 ,其中最直接最有效的方法是构建饱和的基因突变群体 ,通过突变体分析鉴定基因功能。本文主要阐述了各种构建方法及其优缺点以及在功能基因分离鉴定上的应用。自发突变的频率极低 ,且自发突变基因的分离难度比较大 ,只能作为突变群体构建的辅助方法。利用EMS等化学诱变剂可以在短时间内构建大量点突变群体 ,并可用TILLING进行突变检测 ,但多位点的点突变使突变表型难以鉴定。由快中子等物理诱变也可以在短时间内构建大量缺失突变体 ,且可用Deleteagene系统进行检测 ;但多基因缺失、多位点缺失和内含子缺失等使突变表型的分析可能无法进行。利用T -DNA、转座子和反转录转座子等构建插入突变体已经成为突变库构建的主要方法。T -DNA插入已成功应用于水稻大规模突变体的构建 ,但只限于转基因效率较高的品种 ;T -DNA在基因组中整合的复杂性以及转基因过程中由组织培养等引发的突变等 ,增加了突变体表型和分子分析的难度。Tos17是目前应用最为成功的反转录转座子 ,但多拷贝的插入?

Tomato mutants have been used in genetic studies and breeding for decades, yet only a few tomato mutants have been characterized at the molecular level. Similarly, a wealth of sequence information for tomato is now available but the functions of only a few genes are known, New developments-such as the use of saturated mutant populations, new methods for the detection of mutants and new sequence data-are bridging the gap between tomato genes and their functions.

利用番茄突变体进行遗传学研究和育种已有几十年的历史,然而能从分子水平上识别的番茄突变体却为数不多。目前已经获得了大量的番茄序列信息,但仅明确了少数基因的功能。应用饱和突变群体的发展方向,检测突变体和挖掘序列数据的新方法,架起了研究番茄基因和其功能之间的桥梁。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关mutant population的内容
在知识搜索中查有关mutant population的内容
在数字搜索中查有关mutant population的内容
在概念知识元中查有关mutant population的内容
在学术趋势中查有关mutant population的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社