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determination of igm
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  igm的检测
     The system was optimized to realize a reliable determination of IgM in the range of 0.03 μg/mL to 7 μg/mL. It exhibits some advantages, such as simplicity of fabrication, rapidity of measurement, and satisfactory sensitivity.
     实验优化了分析条件下,对IgM的检测范围为0.03μg/mLto7μg/mL,它具有仪器简单、操作方便、灵敏度高等优点。
短句来源
  “determination of igm”译为未确定词的双语例句
     Determination of IgM antibody to Coxsackie B virus in serum and its application in obstetrics
     柯萨奇B组病毒IgM抗体检测方法的建立及在产科中的应用
短句来源
     A Separation-free Amperometric Immunosensor for the Determination of IgM Based on Nafion Modified Electrode
     Nafion膜修饰无分离步骤的IgM电化学免疫传感器研究
短句来源
     Quartz Crystal Microbalance ELISA for Determination of IgM
     石英晶体微天平传感的酶联免疫分析法测定免疫球蛋白IgM
短句来源
     A comparative determination of IgM anti-HBc was done by means of IgM-capture immunoassays in 104 sera of various serologic markers of hepatitis B and 36 NHS with the application of five differentlycoated antibodies,including anti-IgM,anti-μ(Dako),anti-μ(Cappel),anti -μ. (prepared by the authors)and monoclonal IgM antibody.
     本文报道用五种不同的包被抗体(抗-IgM,Dako 抗μ、Cappel 抗μ,本所抗μ和抗μ链单克隆抗体). 经 IgM 捕捉免疫测定法对104份乙型肝炎血清学标志不同的血清和36份正常人血清作了 IgM 抗-HBc 的对比检测,结果揭示本所自制的抗μ具有较高的特异性。
短句来源
     Conclusion The three kits are of good agreement and can alternatively be used for screening and determination of IgM antibodies to Toxoplasma gondii.
     Bi试剂盒与So试剂盒的总符合率为 97.6 %。 结论 这 3种试剂盒都能用于弓形虫IgM抗体的常规普筛和血清学诊断。
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  相似匹配句对
     Determination
     决心
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     DETERMINATION
     决心(英文)
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     The IgM and
     同种异体主动脉与肺动脉移植
短句来源
     Determination of DO
     水中溶解氧的测定
短句来源
     DETERMINATION OF CORD SERA CYTOMEGALOVIRUS IgM
     新生儿脐血抗巨细胞病毒IgM抗体的检测
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  determination of igm
Immunoenzyme assay is developed for determination of IgM- and IgG-antibodies to 6 major human blood phospholipids.
      
Direct enzyme-linked immunosorbent assay that uses enzyme-labeled antigen for determination of IgM antibodies to toxoplasma
      
A modification of the RIA was also useful for determination of IgM antibodies in patients' sera fractionated by sucrose-density centrifugation.
      
We have assessed the comparability of the ELISA tests used for the determination of IgM against GM1 by sending coded serum samples to nine laboratories.
      
Then, the culture supernatant was recovered for the determination of IgM and IgG.
      
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In this paper two cases of primary macroglobulinemia were reported.They were admitted to our associated hospital from 1978-1980, and one ofthem was accompanied by cryoglobulins. Case one, a male of 56, was admitted to our associated hospital onOctober 5, 1978 because of cough for 3 years, palpitation and dyspnea forone year and pallor for two months. On physical examination besidespallor and moist rales in the lungs, the most outstanding feature was thespleno-hepatomegaly. Relevant laboratory findings were:...

In this paper two cases of primary macroglobulinemia were reported.They were admitted to our associated hospital from 1978-1980, and one ofthem was accompanied by cryoglobulins. Case one, a male of 56, was admitted to our associated hospital onOctober 5, 1978 because of cough for 3 years, palpitation and dyspnea forone year and pallor for two months. On physical examination besidespallor and moist rales in the lungs, the most outstanding feature was thespleno-hepatomegaly. Relevant laboratory findings were: sedimentationrate 130 mm/hr., rouleau formation of RBC on blood smears, serum elec-trophoresis by cellulose acetate method showed albumin 30%, globulin α_11.76%, α_2 3.5%, β4.9%, γ_2-γ_3 60% as shown by a deeply stained bandwhich was proven by polyacrylamide electrophoresis to be of the IgMclass: quantitative determination of IgM was 50.8 mg/ml。Due to the factthat the patient had hepatosplenomegaly, myelofibrosis found in marrowsection, and marrow aspiration could not be obtained due to "dry tap", atentative diagnosis of primary myelofibrosis with myeloid metaplasia wasmade. But af1er a large number of lymphocytes were found in the bonemarrow imprint and later on the quantity of IgM was found to be highlyelevated, a revised diagnosis of macroglobulinemia was made .During hospi-talization the patient had been given antitumor treatment which consistedof cyclophosphamide, vincristine, prednisone and later, leukeran. Observa-tion made ten months later showed that the general condition of the patientwas markedly improved, the size of the liver and spleen was reduced,the hemoglobin leyel had elevated from 3 gm/dl to 12.2gm/dl and the IgMlevel dropped from 50.8 mg/ml to 23.37 mg/ml. Case 2, a male of 50, was admitted to our associated hospital on Jan-uary 11, 1980 because of repeated attacks of epistaxis and palpitation for20 years. Four years prior to admission, in addition to the aforementi-oned symptoms, he had cyanosis of his ears, lips and both hands in coldweather, and the process could be reversed upon keeping the body warm.At about the same time, the patient noticed that his vision was declining.Physical examination on admission found the patient was very pale. Bothlungs were hyperresonant. There was no hepatosplenomegaly. Ophthalmosco-pic examination revealed marked engorgement of the retinal veins. X-rayexamination showed basal interstitial pneumonitis bilaterally .Bone marrowsmears showed predominance of lymphocytes 78%, plasma cells 7%, andsome plasmo-lymphoid cells; Bone marrow section showed secondary mye-lofibrosis .Laboratory findings: hemoglobin 4.5 gm/dl, sedimentation rate180 mm/hr., rouleau formation of RBC on blood smears, 2 plus albuminand one plus Bence-Jones protein in urine. Serum protein electrophoresisby the cellulose acetate method: albumin 9.5%, globulin α_1 0%, α_2 2.6%,β14.2%, γ_169.77% being shown by an abnormal deeply stained band, γ_2-γ_33.88%. Immuno-electrophoresis revealed that this abnormal protein was ofthe IgM class, and its quantity was found to be 45.21mg/ml. The serum ofthe patient when exposed to room temperature could be precipitated into awhitish gel-like material which was considered to be cryoglobulins .Immuno-electrophoresis had proven that this cryoglobulin was mainly consisting ofIgM. After admission the patient still had repeated attacks of epistaxis andcommon cold and besides, he had two episodes of fainting and mild con-vulsions .The treatment consisted of intravenous and oral administration ofcyclophosphamide which was followed by long term leukeran; he had twoexsanguination, and five plasmapheresis. Three months after treatmentthe hemoglobin rose up to 8 gm/dl, sedimentation rate had fallen to 37mm/hr., IgM lowered to 32.2 mg/ml, blood viscosity fell from 7.6 to 2.1(normal 1.4--1.8). Unfortunately his vision did not improve much. In the remaining of this paper the experience in making the diagnosis,the possible mechanism of the production of the high viscosity syndromeand bleeding tendency, and the nature of the M protein were discussed.

本文报导了两例原发性巨球蛋白血症,其中1例伴有冷球蛋白血症。详述了血清M蛋白的免疫学与免疫化学检查。并对该病的诊断、临床症状、发病原理,特别是高粘稠综合征及出血原因进行了讨论。

A comparative determination of IgM anti-HBc was done by means of IgM-capture immunoassays in 104 sera of various serologic markers of hepatitis B and 36 NHS with the application of five differentlycoated antibodies,including anti-IgM,anti-μ(Dako),anti-μ(Cappel),anti -μ.(prepared by the authors)and monoclonal IgM antibody.The result exhi bits that anti-μ prepared by the authors has a greater viral specificity than others.Since anti-HBc IgM is detectable only in HBeAg positive and...

A comparative determination of IgM anti-HBc was done by means of IgM-capture immunoassays in 104 sera of various serologic markers of hepatitis B and 36 NHS with the application of five differentlycoated antibodies,including anti-IgM,anti-μ(Dako),anti-μ(Cappel),anti -μ.(prepared by the authors)and monoclonal IgM antibody.The result exhi bits that anti-μ prepared by the authors has a greater viral specificity than others.Since anti-HBc IgM is detectable only in HBeAg positive and HBsAg singly positive sera,it shows no difference from others. However,the detection rate of anti-HBc IgM is very low in anti-HBe positive serum specimens and thus the results are simillar to those done with monoclonal IgM antibody.The detection rates differ apparently between the selfmade anti-li and the others.

本文报道用五种不同的包被抗体(抗-IgM,Dako 抗μ、Cappel 抗μ,本所抗μ和抗μ链单克隆抗体).经 IgM 捕捉免疫测定法对104份乙型肝炎血清学标志不同的血清和36份正常人血清作了 IgM 抗-HBc 的对比检测,结果揭示本所自制的抗μ具有较高的特异性。用本所抗μ仅在 HBeAg 阳性和 HBsAg 单独阳性血清标本中测到IgM 抗-HBc(与其他几种包被抗体无区别);而在抗-HBe 阳性的血清标本中检出率却很低,与用抗μ链单克隆抗体检测结果相似。其结果与其他几种包被抗体有着明显的差异.

We set up a ELISA method for the determination of IgM-, IgG-,IgA-type of rheumatoid factor (RF) and report the result in patients with rheumatoid arthritis (RA), systemic lupus erthematosus (SLE), Sjogren syndrome (SS) and other connective tissue diseases.It was found that the positive rates of IgM-RF 67.1%, IgG-RF 58.6%, IgA-RF42.8%, as well as the OD value in patients with RA were higher than that i n patients with other connective tissue diseases.Our results also revealed the specifcity of ELISA...

We set up a ELISA method for the determination of IgM-, IgG-,IgA-type of rheumatoid factor (RF) and report the result in patients with rheumatoid arthritis (RA), systemic lupus erthematosus (SLE), Sjogren syndrome (SS) and other connective tissue diseases.It was found that the positive rates of IgM-RF 67.1%, IgG-RF 58.6%, IgA-RF42.8%, as well as the OD value in patients with RA were higher than that i n patients with other connective tissue diseases.Our results also revealed the specifcity of ELISA for RF was 2.3%,same as the Latex aggutiniation method. Furthermore.there seemed to be a profound correlation of IgA-RF with SS patients.

用ELISA法建立了检测IgM、IgG、IgA三种类风湿因子。在类风湿关节炎(RA)中IgM-RF,IgG-RF,IgA-RF的阳性率分别为67.1%,58.6%,42.8%,明显高于其他结缔组织病,且OD值也高。ELISA法检测IgM-RF的阳性率明显高于乳胶凝集法检测的阳性率,但特异性一样为2.3%,但弥补了乳胶凝集法只能检测IgM-RF的不足,尤其是建立了IgG-RF和IgA-RF的检测,对临床诊断有很大帮助,我们还发现IgA-RF和SS有一定关系。

 
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