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cysts
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     Cysts of serendipity
     偶然发现的囊肿
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     Intracranial Arachnoid Cysts
     颅内蛛网膜囊肿
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  cysts
Extracellular secretory canaliculi have been described in the hepatoid glands, as well as the richness of hepatoid glands in protein, distribution of hydrophobic lipids in certain hepatoid glands, and formation of excretory ducts and cysts.
      
Patients with organic lesions (tumors, cysts) involving the temporal lobe cortex may reveal more severe spatial hearing disorders than temporal epilepsy patients with the same localization of the foci of convulsive activity.
      
Most of the microorganisms found in the 1- to 3-million-year-old permafrost ground were represented by resting forms (spores, cysts, and cystlike cells with specific organomineral envelopes).
      
We studied formation of epithelial cysts during cultivation of the primary keratinocytes in a collagen gel.
      
Two stages-epithelial spheroid and cysts-can be recognized in the histogenesis process.
      
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The recent opportunity of examining the faecal material of the water-scorpion Leccotrephes japonensis revealed a flagellate which had not previously been described from this host. After fuller investigation I found that it is a species new to science and should be referred to the genus Retortamona; I have named it Retortamonas leccotrephae sp. nov.The specimens of Leccotrephes japonensis used for studies on the parasitic flagellate R. leccotrephae, were collected from ponds on the outskirts of Shanghai, China....

The recent opportunity of examining the faecal material of the water-scorpion Leccotrephes japonensis revealed a flagellate which had not previously been described from this host. After fuller investigation I found that it is a species new to science and should be referred to the genus Retortamona; I have named it Retortamonas leccotrephae sp. nov.The specimens of Leccotrephes japonensis used for studies on the parasitic flagellate R. leccotrephae, were collected from ponds on the outskirts of Shanghai, China. Apart from studying the living flagellates, at the same time stained specimens were prepared for the detailed study of various structures. These specimens were fixed either in Hollande's or in Schaudinn's fluid; stained in Heidenhain's haematoxylin, and destained in 2% aqueous phospho-tungstic acid. Moskowitz' (1950) modification of the Protargol impregnation method was also used for staining.In the living state, the flagellare has a slender, slipper-shaped trunk with an anterior dorsal flexure. The mean body size, exclusive of the posterior spike, is 12.8μ×4.8μ. The length of the slender posterior spike varies from 4.6μ to 15.8μ. There are two flagella of unequal length. The longer one extends forwards and lashes rapidly, causing the animal to rotate and to pursue a special course. The shorter flagellum is directed backwards and usually lies in the cytostome; it moves in an undulating manner.In fixed and stained preparations, the flagellates, not including the posterior spike, show a size-range of 6.2×2.2μto 15.6×5.4μ, with a mean of 11.9×3.4μ. The spike varied from 4.6μ to 13.6μ long, with an average length of 11.2μ. In general, a normal specimen of R. leccotrephae has a slender body; the anterior portion is narrower than the postcerior, and is markedly bent as shown in the living indivi.duals. The posterior portion of the body usually has a dorsal convexity, and the widest region is located near the two-thirds of the body-length from the anterior end.Near that edge of the nuclear membrane lying next to the cytostome, there are two minute, but separate, basal granules which give rise to the two flagella. The anterior flagellum is about as long as the body proper: much longer and more slender than the posterior flagellum, which usually lies within the cytostome, and commonly shows a series of two or three undulations; it often stains more deeply than the anterior flagellum.In hematoxylin preparations the nucleus is well stained, and clearly shows a layer of chromatin granules lying against the nuclear membrane, and a large central endosome, composed of a mass of granules.The cytostome is a large structure in this animal. It usually occupies about twothirds of the total length of the body proper. Along the margin of the cytostome there are two deeply staining fibres, the one on the right margin is longer than that on the left.Both the precystic forms and cysts of R. leccotrephae have been observed. The body of precystic individuals which are preparing to encyst, becomes smailer and rounds up; meanwhile, the chromatin of the nucleus condenses and forms a deeply staining endosome. The anterior flagellum is still disposed freely, and no cyst wall has as yet been formed. The border-line of the cytostome is distinct, but the two basal granules are visible only in favourable preparations. The mature cysts are nearly peanut-shaped, and are about twice as long as wide. In the stained specimens they are about 4.6μ long and 2.4μ wide. The cyst wail is of uniform thickness, and the nucleus is visible at one end. The other conspicuous structures within the cyst are the two flagella and the cytostomial fibres, which are arranged as they are in the trophozoite, except that the anterior flagellum is here directed posteriorly.DISCUSSION & SUMMARYIn its general body-form, R. leccotrephae closely resembles Mackinnon's R. agilis ('11) from crane-fly larvae, and also Corradett's R. gryllotalpae ('37) from the mole cricket, but it is especially like Geiman's R. caudacus ('32) from the aquatic larvae ef certain beetles.

红娘华蛐的身体结构,一般与Mackinnon所述的R.(Embadomonas) agilis和Corradetti所述的R.grillotalpae相似,特别近似Geiman所述的R.caudacus(图15,16)。但红娘华蛐有大约等於体长2/3的大胞口,和长於或等於体长的针状尾巴,而且无论在生活时或固定染色後,头部均向背後扭曲(此较图1和15)。这些特性,显然与过去文獻中所记载的种类不同,故(氵夬)定为蛐属——新种。

In 1955, a specimen of sporozoa was found in the subcutaneous tissue of slaughtered cattle, The cysts of these sporozoa were whitish in color, measuring from 0.13 to 0.23 mm in diameter, and were found to be located chiefly around the blood vessels among tne connective tissues. The cyst wall, measuring 18,25-36.50 μ. in thickness, consisted of 3 layers. Inside the cyst wall, numerous oval or spindle-shaped spores were densely packed. When liberated from the cyst, the spores were observed...

In 1955, a specimen of sporozoa was found in the subcutaneous tissue of slaughtered cattle, The cysts of these sporozoa were whitish in color, measuring from 0.13 to 0.23 mm in diameter, and were found to be located chiefly around the blood vessels among tne connective tissues. The cyst wall, measuring 18,25-36.50 μ. in thickness, consisted of 3 layers. Inside the cyst wall, numerous oval or spindle-shaped spores were densely packed. When liberated from the cyst, the spores were observed to move slowly under the microscope. Morphologically, the parasite is identical with Besnoitia besnoiti, which was first discovered by Besnoit and Robin in 1912, and for which a new genus was created by Franco and Borges in 1916. As hitherto the distinction between Besnoitia, Sareocystis and Globidiwm has not yet been agreed upon by different authors. The characteristics of the 3 genera were reviewed and compared with those of the present specimen. After careful study, it was revealed that Besnoitia differed from Sareocystis in the morphology of the parasite, the tissue parasitized as well as in pathogenicity. Likewise it differed from Globidium in the tissue parasitized and in its method of reproduction. It was therefore concluded that this parasite should belong to the genus Besnoitia Franco and Borges, 1916, and it should be known as Besnoitia besnoiti Marotel, 1912.Comparison of Besnoitia besnoiti and Sareocystis hirsuta of the cattle as observed by the writer and recorded in the literature is given below:

1.本文报告了1955年在北京屠宰场内一只黄牛的皮下组织里发现的孢子虫—贝氏贝诺孢子虫。 2.在本例所见的贝氏贝诺孢子虫,孢子囊为白色,直径为0.13—0.23毫米,成群的分布在皮下的结缔组织里。它们与血管有密切的关系。囊壁厚(18.25—36.50微米),分为三层,囊内含有一团孢子。孢子呈椭圆形或梭形。新鲜的孢子可以缓慢活动。孢子对大白鼠或小白鼠无感染力。 3.本文详述了贝诺孢子虫、住肉孢子虫和球形体三属的特点和三属的区别。贝诺孢子虫在形态上、寄生部位上和动物的自然感染率上,都和住肉孢子虫不同;在寄生部位上和繁殖方法上又和球形体不同,所以贝诺孢子虫应自成一属。文中并将过去文献中,与贝诺孢子虫形态相同的种类,加以比较确定后,均归於贝诺属内。

The discovery of the larval form of the species of Asymphylodora Looss (1899) dated back to the time of von Baer (1827), who described Cercaria paludinae impurae, De Filippi (1854) recorded from the same snail host a distome bearing the same name. For one and half century knowledges regarding the developmental life history of the well-known type of the genus, A. tincae, have gradually accumulated. Small as it is, the worm, however, has received the attention of many a distinguished helminthologists such as Diesing...

The discovery of the larval form of the species of Asymphylodora Looss (1899) dated back to the time of von Baer (1827), who described Cercaria paludinae impurae, De Filippi (1854) recorded from the same snail host a distome bearing the same name. For one and half century knowledges regarding the developmental life history of the well-known type of the genus, A. tincae, have gradually accumulated. Small as it is, the worm, however, has received the attention of many a distinguished helminthologists such as Diesing (1858), Looss (1899). Lithe (1809), Fuhrmann (1916), Dubois (1909), Wesenberg-Lund (1934) and Skrjabin (1955). The recent work of Deblock, Capron and Biguet (1957) elucidated the life cycle of a new subspecies, A. tincae var. mediagraba, while other workers like Serkova and Bykhovskii (1940), Biguet, Deblock and Capron (1956) and Stunkard (1959) described the development of several progenetic species, The significance of progenesis to the phylogeny of Digenea is discussed by Stunkard (1959). Inspire of the above-mentioned important advances on the knowledge of this genus, there still remain much to be worked out regarding the biology of this group.The present communication repoorts life history studies on Asymphylodora macostoma Ozaki, 1925 and A. japonica Yamaguti, 1928.The adults of A. macrostoma were obtained from Puntia sp. (Cyprinidae) occurring in the mountain stream of Yungan, Central Fukien. Their structure and measurements were described in detail. They are indistinguishable from the original description of Czaki (1925) and Yamaguti (1938). The molluscan intermediate host of A. macrostoma in Fukien is Melania peregrinorum Heude inhabiting the mountain stream among rocks and under stones.The sporocyst stage was not discovered in natural infection. The second generation redia measures 1.39mm in length and 0.274mm in width. The gut contains numerous brownish granules derived from the host tissue. The general shape of the redia is sac-like. It possesses no muscular feet. In the body of a mature redia there are 5 or 6 cercariae and some germ-balls observed.The cercariaeum is a comparatively large distomate larva 0.3-0.37mm in length and 0.11-0.13mm in width. It is brownish yellow ia color especially in its posterior part. The cuticle is covered with spines distributed in transverse rows. The oral sucker measures 0.08-0.09mm by 0.09-0.097mm in diameter. The ventral sucker is smaller, measurihg 0.068 in diameter. On the dorsal wall of the oral sucker there are four rows of short conspicuous spines lining two-thirds of the inner surface of the sucker. There are also 3-8 big flat spines attached to the inner surface. The acetabulum is also armed with small conspicuous spines on its entire inner surface, Such spinulation is not present in the cercariaeum of A. japonica but is rather similar to that described in Cercariaeum squamosum by Deblock, Carpron and Biguet (1957). The oral sucker is followed by a short prepharynx, which leads to a globular pharynx. The esophagus is long, bending several times and bifurcating in front the acetabulum into two intestinal caeca. On both sides of the esophagus four bundles of penetration glands are present, occupying the area between the pharynx and acetabulum. There are altogether 40-42 unicellular gland cells. Four bundles of ducts proceed anteriorward along the medial and lateral regions to arrive at and open on the inner dorsal wall of the oral sucker. The penetration glands become graduallv diminished as the cercariaeum grows more mature, so that in the adolescaria stage their contents are greatly reduced. The posterior tip of the body is armed with a number of long and sharp spines, which probably help the larva in its creeping movement.The excretory system is complicated. Since the body of the cercariaeum is full of cystogenous cells, which obscure the capillary tubules connecting the flame cells, their arrangement cannot be traced, and yet when the cercariaeum has encysted, while still in the snail host, most of the gland cells have disappeared rendering the tiny excretory tubules observable. The excretory bladder is a sinuous tube, making one or two left and right bendings as it advances anteriorward. The bladder is lined with a series of large epithelial cells. From the anterior aspect of the bladder, there arise two collecting tubules, which extend obliquely foreward to both lateral fields. They continue to advance to the level of esophagus and then turn posteriorly to about the mid region of the whole length of the collecting tubule and divides into two branches. The anterior branch gives off branches two times resulting in three groups of solenocytes. The first group consists of five cells, while the other two have three calls cach. The posterior branch divides into two main sub-branches with seven and three flame cells in each group. The total number of flame cells is about 42. The pattern of their arrangement can be better understood by tracing the development of the excretory system from the germ-ball to the mature cercaria. Four stages were observed:1. In the early germ-ball stage, when the oral sucker and the phraynx are being differentiated, the collecting tubulesare formed. They are connected to a small bladder situated at the posterior end of the body. The two tubules having passcd anteriorly and reached about two-thirds of the body length, make a characteristic loop and divide into an anterior and a posterior branch Their arrangement can be depicted as 2(1+1)=4. 2. In the second stage, when the ventral sucker is formed, both the anterior and posterior branches divide into three flame cells cach. The formula is reprsented as 2(3+3)=12.3. In the third stage the division of the anterior branch into three smaller branches is witnessed. The posterior branch is not subdivided. It still possesses three solenocytes. The formula of arrangement is 2[(2+2+4)+(2+1)]=22.4. The fourth or the cercaria stage shows great increase of cells, especially in the posterior branch. Their arrangement are indicated in the foregoing description. The formula can be expressed as follows: 2[(3+3+5)+(3+3+4)]=42.It was observed that the number of cells and the pattern of their arrangement are not exactly homologous between the left and right sides of the body. The above description indicates that the cell formula is constant only in relative sense that is they differ in different stages of development.Specimens of Asymphylodora japonica were secured from Pscudorasbora parva (Temm. aud Schle.) and also from Cyprinus carpio L. The structure and measurements of the adult are fully described. They resemble the original description of Yamaguti's closely. A. japonica develops in Parafossarulus eximius (Frauenfeld) and P. striatulus (Benson). Both molluscs inhabit the ponds and rivulets in Foochow area. Dissections of the snails reveal stages of rediae and cercariae. Spororcysts were not found in the natural infection. The second generation redia is elongated in shapeIt measures 1.5mm in length and 0.45mm in transverse diameter. The fully mature redia contains seven to eight cercariae in its body.The cercariaeum is elongated or spindle-shaped, measuring 0.5-056mm in length and 0.16mm in greatest width. The cuticle is armed with spines transversely arranged. Oral sucker is round in shape with a diameter of 0.1mm. Ventral sucker, larger than the oral sucker, has a diameter of 0.12-0.13mm. There is a short prepharynx followed by a glubose pharynx. The esophagus is long. It bifurcates in front of the acetabulum into two intestinal caeca, which extend posteriorly to one fourth of the body length from the hind extremity. Four groups of unicellular penetration glands occupy the region between the oral and ventral suckers, numbering altogether about 36-38. Four bundles of gland-ducts lead forward and open on the inner dorsal wall of the oral sucker. The excretory system resembles that of cercariaeum of A. macrostoma. The excretory bladder is a long sinuous tube, similar to that of cercariaeum A. macrostoma.The metacercariae of A. japonica, probably in the pre-encystment stage, were frequently encountered in the snail host. It is larger in size than the cercariaeum, measuring 0.8-1.00mm in length and 0.4mm in greatest width, Oral sucker 0.038-0.11mm in diameter, is smaller than acetabulum, The later is 0.132-0.149 mm in diamcter. The oral sucker is smaller than acetabulum. The later is 0.132-0.149 mm in diam- eter. The oral sucker is followed by a prepharynx, which is connected to a glubose pharynx 0.049-0.50mm in diameter. The esophagus is long It bifurcates before the ventral sucker into two intestinal caeca extending to the hind end of the testis. The genital organs alrsady begin to develop. A single oval testis, measuring 0.30 by 0.20mm is situated at the posterior part of the body. Immediately anterior to the testis, an ovary triangular or oval in shape is present. Its diameter is 0.10mm. On the right side of the acetabulum the primordium of the cirrus pouch and metraterm appear as two columns of nuclei. The metacercariae can encyst in the same snail host. They can also migrate to another snail of the same species. The cyst measures 0.332-0.365mm in diameter. Under cover-glass pressure, it measures 0.500mm in diameter.Experiments were performed to infect Puntia sp., secured from places where no Melanin snails were found, and members of which were found to be free from infection, by feeding them with from Melanin peregrinorum. Fifteen days after infection, the fishes were dissected and adult worms similar to A. macrostoma were found. One experimentally infected fish died five days after infection wite forth immaure worms found. They were all very similar in size and development. Experiments were also performed to secure adult A. japonica by feeding laboratory-reared gold fishes (Carassius auratus) with cysts from Parafossarulus exiraius (F.) Fifteen days after infection, ad

1.福建省的两种側殖吸虫,巨口侧殖吸虫(Asymphylodora macrostoma Ozaki,1925)及日本侧殖吸虫(A. japonica Yamaguti,1928)的生活史均經闡明。2.巨口侧殖吸虫的终末宿主为刺鲃(Punctius sp.),貝类的中間宿主为川蜷贝(Melania peregrinorum Heude)。日本侧殖吸虫的终末宿主为麦穗魚(Pseudorasbora parva (T. and S.)),及鯉魚(Cyprinus carpio L.),貝类中間宿主为两种的纹沼螺(Parafossarulus eximius (Fruenfeld)及P. striatulus (Benson))。3.幼虫各期的形态經观察和叙述,特別关于排泄系統的构造經詳細的探討。4.两种侧殖吸虫幼虫期的形态,特别关于排泄囊的构造以及穿刺腺的存在,与侧殖Asymphylodora Looss,1899原属有很大的不同,作者建議创立一个新属Orientotrema Tang,1962 Gen. Nov.借以容納有管状排泄囊的种类。属的特征經叙述,末后并附侧殖亚科(Asymphylodorinae)各属...

1.福建省的两种側殖吸虫,巨口侧殖吸虫(Asymphylodora macrostoma Ozaki,1925)及日本侧殖吸虫(A. japonica Yamaguti,1928)的生活史均經闡明。2.巨口侧殖吸虫的终末宿主为刺鲃(Punctius sp.),貝类的中間宿主为川蜷贝(Melania peregrinorum Heude)。日本侧殖吸虫的终末宿主为麦穗魚(Pseudorasbora parva (T. and S.)),及鯉魚(Cyprinus carpio L.),貝类中間宿主为两种的纹沼螺(Parafossarulus eximius (Fruenfeld)及P. striatulus (Benson))。3.幼虫各期的形态經观察和叙述,特別关于排泄系統的构造經詳細的探討。4.两种侧殖吸虫幼虫期的形态,特别关于排泄囊的构造以及穿刺腺的存在,与侧殖Asymphylodora Looss,1899原属有很大的不同,作者建議创立一个新属Orientotrema Tang,1962 Gen. Nov.借以容納有管状排泄囊的种类。属的特征經叙述,末后并附侧殖亚科(Asymphylodorinae)各属檢索表的修訂。5.本类吸虫的生活史及习性問题經詳細討論。

 
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