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   rb gene mutation 的翻译结果: 查询用时:0.008秒
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眼科与耳鼻咽喉科
肿瘤学
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rb gene mutation
相关语句
  rb基因突变
     Analysis of Rb gene mutation in squamous cell carcinoma of larynx
     喉鳞癌组织中Rb基因突变的分析
短句来源
     Results:(1) The mutation ratio of p53 in ovarian cancer was 32%, (2) The mutation of Rb gene in ovarian cancer was 21%, (3)Neither p53 nor Rb gene mutation ratio was found significant difference in varions differential stages.
     结果发现p53基因可能的突变率为32%,Rb基因可能的突变率为21%,不同分化程度肿瘤的p53及Rb基因突变率未见明显区别。
短句来源
     Conclusion:RB gene mutation takes part in the development of laryngeal carcinoma.
     结论 :RB基因突变在喉癌的发生发展中起着一定的作用 ;
短句来源
     Conclusion: Non functional Rb protein produced by gene mutation was one cause in the development of laryngeal neoplasma, howerver, Rb gene mutation was not common in pathogenesis of human laryngeal neoplasma.
     结论 :Rb基因突变导致的喉癌细胞不能产生有功能的 Rb蛋白可能是喉癌发生的原因之一 ,但 Rb基因的突变并不是喉鳞癌的常发事件。
短句来源
     RFLPs and VNTRs within the Rb gene were used as genetic markers for haplotype analysis. Results The probands from 79 RB kindreds were identified to have Rb gene mutation, including 25 somatic mutations and 54 germline mutations (36 new germline mutations, 15 inherited mutations and 3 mosaicisms). The WBC DNAs from their family members were also analyzed for determining origin and carrier of mutation.
     结果79个有Rb基因突变的RB家系中25例先证者仅查出体细胞起源的Rb基因突变,其余54例存在生殖细胞起源的Rb基因突变,其中36例突变是新产生的,15例突变由亲代遗传而来,此外,尚有3例Rb基因突变嵌合体。
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  rb基因变异
     The overall incidences of Ha-ras, p53 and RB gene mutation and HPV infection in tumor were 61.5% ,36% ,30.8% and 15.4% , respectively.
     结果表明,膀胱癌组织中HPV16、18感染率及Ha-ras、p53、RB基因变异率分别为61.5%、36%、30.8%及15.4%;
短句来源
  “rb gene mutation”译为未确定词的双语例句
     Preliminary analysis of p53 gene and Rb gene mutation in ovarian cancer tissue.
     p~(53)基因与Rb基因在卵巢癌组织中突变的初步分析
短句来源
     Results Only did the polymorphisms, silent mutations and variants in intron have been shown in p53 gene, no real p53 gene mutation was found in retinoblastoma no matter the tumor carried Rb gene mutation or not.
     结果52例Rb肿瘤中除可见多态性、同义突变及内含子序列的变异外,未检出p53基因有真正的点突变。
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Objective To develop a diagnostic test for direct identification of disease causing mutation in the patients with retinoblastoma and correct prediction of carrier status in unaffected adults and newborns in the RB kindred.Methods Southern blot hybridized by Rb cDNA and other intragenic probes were used to detect big detetions or rearrangements at Rb gene locus. SSCP analysis and direct sequencing of primer directed enzymatic amplification to identify point mutations as small...

Objective To develop a diagnostic test for direct identification of disease causing mutation in the patients with retinoblastoma and correct prediction of carrier status in unaffected adults and newborns in the RB kindred.Methods Southern blot hybridized by Rb cDNA and other intragenic probes were used to detect big detetions or rearrangements at Rb gene locus. SSCP analysis and direct sequencing of primer directed enzymatic amplification to identify point mutations as small as a single nucleotide change. RFLPs and VNTRs within the Rb gene were used as genetic markers for haplotype analysis.Results The probands from 79 RB kindreds were identified to have Rb gene mutation, including 25 somatic mutations and 54 germline mutations (36 new germline mutations, 15 inherited mutations and 3 mosaicisms). The WBC DNAs from their family members were also analyzed for determining origin and carrier of mutation.Conclusion The direct identification of causing cancer mutations by combining SSCP analysis and direct DNA sequencing showed many advantages than other indirect methods such as haplotype analysis. It can distinguish hereditary RB from nonhereditary RB and identify the unaffected carriers without family history and informes affected family member. This method is helpful in gene diagnosis and genetic counselling.

目的建立Rb基因突变的基因诊断方法,正确估计视网膜母细胞瘤(RB)患者的预后及其家庭成员的患病风险。方法综合利用Southernblot杂交、SSCP分析、直接DNA序列测定等多种分子生物学技术作染色体单体型分析及Rb基因点突变的直接检测。结果79个有Rb基因突变的RB家系中25例先证者仅查出体细胞起源的Rb基因突变,其余54例存在生殖细胞起源的Rb基因突变,其中36例突变是新产生的,15例突变由亲代遗传而来,此外,尚有3例Rb基因突变嵌合体。结论直接检测Rb基因点突变可不依赖于患者家庭成员RB发病情况的遗传背景资料诊断患者是否属于遗传型,正确估计患者的预后;并能在肿瘤发生前甚至产前检出Rb基因突变携带者,正确估计患病风险

To clarify the clinical significance of alterations of Ha-ras, p53 and RB gene as well as infection with HPV16,18 in human bladder cancer, we examined the state of Ha-ras, p53 and RB genes, sequences of HPV16/18, and their association with clinicopathological parameters in 39 cases of bladder cancer and 7 cases of normal tissue, using nonisotopic PCR-SSCP and dot blot. The overall incidences of Ha-ras, p53 and RB gene mutation and HPV infection in tumor were 61.5% ,36% ,30.8% and...

To clarify the clinical significance of alterations of Ha-ras, p53 and RB gene as well as infection with HPV16,18 in human bladder cancer, we examined the state of Ha-ras, p53 and RB genes, sequences of HPV16/18, and their association with clinicopathological parameters in 39 cases of bladder cancer and 7 cases of normal tissue, using nonisotopic PCR-SSCP and dot blot. The overall incidences of Ha-ras, p53 and RB gene mutation and HPV infection in tumor were 61.5% ,36% ,30.8% and 15.4% , respectively. The HPV positive rate was negatively correlated with clinical stage and pathological classification. Rather, the mutations of Ha-ras and p53 gene were positively correlated with the above clinical parameters. The incidence of Ha-ras gene mutation in recurring tumors was significantly higher than that in primary ones. A negative correlation between HPV infection and p53 mutation was also found. The results suggest that the above molecular events and their interaction play important roles in the development of bladder cancer, and that they wonld be of practical assistance in the prognosis and monitoring of bladder cancer.

采用分子杂交、PCR-SSCP技术检测了39例膀胱癌及7例正常膀胱组织中HPV16、18感染及Ha-ras、p53、RB基因变异,并结合临床分期、病理分级等参数进行了综合分析.结果表明,膀胱癌组织中HPV16、18感染率及Ha-ras、p53、RB基因变异率分别为61.5%、36%、30.8%及15.4%;HPV阳性率与分级分期、p53基因突变与HPV感染呈明显负相关;Ha-ras、p53基因突变率与分级分期正相关,其中Ha-ras基因在复发肿瘤的突变率显著高于原发肿瘤;部分标本同时呈HPV感染与p53基因突变、Ha-ras与p53基因突变.提示高危HPV感染和Ha-ras、p53、RB基因变异及相互作用在很大程度上参与了膀胱癌的发生及演变,并与其生物学行为密切相关,有望成为判断肿瘤恶性程度、自然病程及预后的估价指标.

Objective: To recognize the mutation status of p53 and Rb cancer suppresor genes. Methods: for the first time, 57 ovarian cancer samples were analysised by PCR for DNA amplification, and then SSCP, furthes PCR DNA sequence. Results:(1) The mutation ratio of p53 in ovarian cancer was 32%, (2) The mutation of Rb gene in ovarian cancer was 21%, (3)Neither p53 nor Rb gene mutation ratio was found significant difference in varions differential stages. Conclusions:...

Objective: To recognize the mutation status of p53 and Rb cancer suppresor genes. Methods: for the first time, 57 ovarian cancer samples were analysised by PCR for DNA amplification, and then SSCP, furthes PCR DNA sequence. Results:(1) The mutation ratio of p53 in ovarian cancer was 32%, (2) The mutation of Rb gene in ovarian cancer was 21%, (3)Neither p53 nor Rb gene mutation ratio was found significant difference in varions differential stages. Conclusions: PCR SSCP DNA sequence method is the best one in testing gene mutation for its simplicity, sensitivity and no radioisotope contamination. Direct PCR DNA sequence can test point mutation of single amino acid change.

为了解卵巢癌组织中抗癌基因p53与Rb基因的突变情况,我们把PCR单链构象多态(PCR-SSCP)银染技术及PCR-DNA直接测序方法应用于57例卵巢癌组织细胞的检测。结果发现p53基因可能的突变率为32%,Rb基因可能的突变率为21%,不同分化程度肿瘤的p53及Rb基因突变率未见明显区别。结论,PCR-SSCP银染技术是筛查基因突变简便、敏感、无核素污染的最佳方法,而PCR-DNA直接测序又能精确到检测单个核苷酸的突变及突变内容

 
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