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central complex design method
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This paper is about the study on the strain of Alcaligenes sp.NX-3 which could produce a new type of biopolymer PS-238. PS-238 has the excellent stability towards temperature,pH and salts. It belongs to the family of microbial polysaccharides with a wide range of industrial applications. Studies on the nutritional requirement for optimal polysaccharride production were carried out. The carbon source and nitrogen source were first investigated respectively. Then central complex design method was applied...

This paper is about the study on the strain of Alcaligenes sp.NX-3 which could produce a new type of biopolymer PS-238. PS-238 has the excellent stability towards temperature,pH and salts. It belongs to the family of microbial polysaccharides with a wide range of industrial applications. Studies on the nutritional requirement for optimal polysaccharride production were carried out. The carbon source and nitrogen source were first investigated respectively. Then central complex design method was applied for the optimization of cultural medium. It was shown that carbon source was the most remarkable factor for the production of PS-238. The concentration of K_2HPO_4 was aslo very important. By using the optimized medium, the yield of PS-238 increased from 10.78?g/L to 22.85?g/L

一株产碱杆菌AlcaligenesspNX3可分泌一种新型高分子多糖PS238,该多糖具有耐高温、耐酸碱、耐盐等优良特性。碳源、氮源等培养基组分对该多糖产量有重要的影响。文中采用单因素的实验方法确定了发酵培养基的碳源、氮源分别为蔗糖和蛋白胨,然后采用中心复合设计法对培养基的主要成分进行了分析和优化。结果表明,碳源是极显著的因素,K2HPO4次之。最优发酵培养基配方为,蔗糖浓度49g/L,蛋白胨浓度65g/L,K2HPO42g/L,MgSO403g/L,根据预测的最优发酵水平的营养配比,产量由1078g/L提高至2285g/L。

Microorganism growth is greatly effected by carbon sources , nitrogen sources, vitamin and metal ions. With the investigation of single factor, the components of carbon source and nitrogen source were firstly designed respectively , and the optimized cultural medium was decided by the central complex design method then, with which the prediction of the optimized medium has been made for the culture of B. licheniformis NG521-1. Fermentation was conducted with the predicted medium, the grown population of...

Microorganism growth is greatly effected by carbon sources , nitrogen sources, vitamin and metal ions. With the investigation of single factor, the components of carbon source and nitrogen source were firstly designed respectively , and the optimized cultural medium was decided by the central complex design method then, with which the prediction of the optimized medium has been made for the culture of B. licheniformis NG521-1. Fermentation was conducted with the predicted medium, the grown population of bacteria per unit broth increased sharply, from 3.5×10~7 cfu/mL up to 1.2×10~8 cfu/mL.

碳源、氮源、各种维生素和金属离子对微生物培养有重要影响。先采用单因素,后采用复合因素的方式,确定碳源和氮源,然后采用中心复合设计分析法对地衣芽孢杆菌(Bacilluslicheniformis)NG521-1的培养基主要成分进行分析和优化,根据预测最优发酵水平的配比,调整培养基,使发酵液中菌体生长量大大提高,由原来0 35×108个/mL提高到1 2×108 个/mL。

 
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