助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   gamma t cell receptor gene rearrangement 的翻译结果: 查询用时:0.006秒
图标索引 在分类学科中查询
所有学科
心血管系统疾病
肿瘤学
更多类别查询

图标索引 历史查询
 

gamma t cell receptor gene rearrangement
相关语句
  相似匹配句对
     On the Extension of Structured Gamma
     关于并行语言Gamma的结构化扩充
短句来源
     Gamma-Ray Bursts
     γ射线暴研究概况
短句来源
     Green's equivalences on gamma rings
     Γ-环上的Green等价
短句来源
     Gamma Knife Radiosurgery for Craniopharyngiomas
     颅咽管瘤的伽玛刀治疗
短句来源
     The Characters and Application of Generalized Gamma Distribution
     广义Γ分布的特性和应用
短句来源
查询“gamma t cell receptor gene rearrangement”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
没有找到相关例句


Objective To detect acute lymphoblastic leukemia's (ALL's)minimal residual disease(MRD) rapidly and effectively. Methods In this assay, the gamma T cell receptor gene rearrangements serve as marker genes. The gene rearrangements are amplified from the diagnostic specimens using a consensus V segment primer and a consensus J segment primer to which the promoter T7 RNA polymerase has been appended. The PCR product from this amplification is transcribed...

Objective To detect acute lymphoblastic leukemia's (ALL's)minimal residual disease(MRD) rapidly and effectively. Methods In this assay, the gamma T cell receptor gene rearrangements serve as marker genes. The gene rearrangements are amplified from the diagnostic specimens using a consensus V segment primer and a consensus J segment primer to which the promoter T7 RNA polymerase has been appended. The PCR product from this amplification is transcribed into a radiolabeled RNA probe. The opposite DNA strand is transcribed into test RNA from the PCR product of different staged specimens. The test RNA is hybridized with the probe, and later the digestion with RNase A, Polyacrylamide gel electrophoresis and autoradiography are in progress. Results According to the mechanism, the perfectly matched RNA duplex can prevent the digestion of RNase A, and the presence of the leukemia cells in the test specimen can be determined. Logarithmical dilution experiments with DNA of a cell line from ALL have shown that this assay's sensitivity is at the 10 -5 level. Minimal residual disease was successfully detected in a case of ALL during its complete remission stage. But if the probe and test RNA are not from the same individual, the results of this kind of cross hybridization are negative. Conclusion The above results suggest that this assay can become an effective measure in the detection of ALL MRD clinically.

目的为了快速、敏感地检测急性淋巴细胞白血病(急淋)微小残留病。方法以T细胞受体γ链基因重排为标志基因,将确诊时标本扩增后的PCR产物转录成放射标记的RNA探针,而不同病期标本的PCR产物转录成待测RNA,待测RNA与探针杂交后经RNaseA消化,之后再经聚丙烯酰胺凝胶电泳和放射自显影。结果急淋细胞系DNA对数稀释试验表明本方法的敏感度在10-5水平。1例急淋完全缓解期微小残留病也被成功地检测出来。而当探针和待测RNA并非来自同一个体时,交叉杂交的结果为阴性。结论为临床检测急淋微小残留病提供了一种有效手段

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关gamma t cell receptor gene rearrangement的内容
在知识搜索中查有关gamma t cell receptor gene rearrangement的内容
在数字搜索中查有关gamma t cell receptor gene rearrangement的内容
在概念知识元中查有关gamma t cell receptor gene rearrangement的内容
在学术趋势中查有关gamma t cell receptor gene rearrangement的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社