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concentrate effect
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A new kind of polyacrylamide gel electrophoresis, current density gradient gel electrophoresis(CDGGE), has been developed. CDGGE shows good concentrating effect not only at the beginning of electrophoresis, but also during the run, Moreover, with the continuous system employed in CDGGE, possible harmful effects resulting from discontinuities are avcided.

对聚丙烯酰胺凝胶电泳的浓缩效应作了研究。建立了电流密度梯度聚丙烯酰胺凝胶电泳(CDGGE)。CDGGE不仅在电泳开始时,而且在电泳过程中对被分离的样品有压缩作用,从而提高了分辨率。作者用CDGGE法将面包酵母混合转移核糖核酸分成20条带,并对其中的一些带作了鉴定。

Laboratory observations were made on the number of Wuchereria bancrofti microfilariae ingested and the number developing into infective larvae in Culex pipiens pallens. Mosquitoes were fed on blood of carriers with three levels of microfilariae density, namely: ultra-low density(10 mff per 1 ml venous blood which was undetectable with conventional diagnostic techniques), low density (about 5 mff per 60 μl of peripheral blood) and moderate density (10 mff per 60 μl of peripheral blood). Experiments were carried...

Laboratory observations were made on the number of Wuchereria bancrofti microfilariae ingested and the number developing into infective larvae in Culex pipiens pallens. Mosquitoes were fed on blood of carriers with three levels of microfilariae density, namely: ultra-low density(10 mff per 1 ml venous blood which was undetectable with conventional diagnostic techniques), low density (about 5 mff per 60 μl of peripheral blood) and moderate density (10 mff per 60 μl of peripheral blood). Experiments were carried out for comparing the number of microfilariae ingested by mosquitoes with that of circulating microfilariae of carriers in same volume of blood, there was no statistical significant differences (p>0.05). Results showed that Culex pipiens pallens fed on blood of carriers with above mentioned levels of density of microfilariae domonstrated no concentrating effect. However, the proportion of infective larvae increased as the microfilarial density of donors increased. The maturity rate of ingested microfilariae in mosquitoes fed on carriers with three levels of microfilariae density was 11.5, 37.1 and 45% respectively. It is evident that the relationship between Culex Pipiens pallens and periodic W. bancrofti in China is quite different from that between Aedes polynesiensis and subperiodic W. bancrofti in Pacific region. This finding would probably be beneficial to the consolidation of the achievement of bancroftian filariasis control in our country.

本文于1985年在国内开始应用计数蚊胃摄入微丝蚴数与供血者同量末梢血微丝蚴数相比较的方法,观察淡色库蚊吸血时的微丝蚴摄入量及幼丝虫发育成熟率。以3组共16例不同密度微丝蚴血症者作供血源,即超低密度(60μl末梢血查不出微丝蚴,1ml静脉血微丝蚴10条以下)7例、低密度(60μl末梢血微丝蚴5条左右)5例,和中等密度(60μl末梢血微丝蚴数10条)4例,淡色库蚊吸血时摄入微丝蚴数与末梢血微丝蚴数相比较,经统计学分析均无显著差异(t分别为0.667、0.791和0.014.p均大于0.05);3组淡色库蚊幼丝虫发育成熟率依次为11.5%、37.1%和45%。以上结果提示,淡色库蚊吸血时摄入微丝蚴未见浓集现象,而幼丝虫发育成熟率则随供血者微丝蚴密度增高而增高。

The polyaciylamide gel discoid shape elutrophoresis detecting CSF oligoclonal IgG bands has been served as a qualitative index which reflects the abnormality of the CNS humoral immunity. The method has an electric charge effect,a concentrating effect as well as molecular sieve effect and so on. There- fore it has high resolving power and has no need for concentrating CSF. Only 200μg protein CSF relects sensitively the changes in the quantity and quality of the γ-globulins. Especially,it...

The polyaciylamide gel discoid shape elutrophoresis detecting CSF oligoclonal IgG bands has been served as a qualitative index which reflects the abnormality of the CNS humoral immunity. The method has an electric charge effect,a concentrating effect as well as molecular sieve effect and so on. There- fore it has high resolving power and has no need for concentrating CSF. Only 200μg protein CSF relects sensitively the changes in the quantity and quality of the γ-globulins. Especially,it can display clearly oligoclonal IgG bands. Authors have used the method to detect the CSF samples of 124 cases and the results showed that the method can be of high sensitivity and have certain clinical practical val- ue.

聚丙烯酰胺凝胶盘状电泳法检测 CSF 寡克隆 IgG 区带已作为反映中枢神经系统体液免疫异常的定性指标。聚丙烯酰胺凝胶盘状电泳方法作 CSF 寡克隆 IgG 区带分折分辨力高,不需要浓缩 CSF,只要200μg 蛋白的 CSF 就可灵敏地反映出γ-球蛋白质和量的变化.作者应用本方法对124例 CSF 标本检测的结果表明:该法操作简便、灵敏度高,具有一定的临床实用价值。

 
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