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human fetal bone
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  人胎儿骨骼
    The construction of rapid amplification of cDNA ends cDNA libraries from human fetal bone and joint
    人胎儿骨骼和关节RACE cDNA文库的构建
短句来源
    Objective To construct rapid amplification cDNA ends(RACE) cDNA libraries from human fetal bone and joint and provide resources for isolation of bone and joint specific development related genes.
    目的 建立人胎儿骨骼和关节快速扩增 c DNA末端 (rapid amplification of c DNA ends,RACE c DNA)文库 ,为分离骨骼和关节发育相关基因奠定基础。
短句来源
    Results A protocol for RACE cDNA library construction from bone and joint was established and two RACE cDNA libraries from human fetal bone and joint were successfully constructed.
    结果 建立了从骨骼和关节构建 RACE c DNA文库的方法 ,并用该方法成功地构建了人胎儿骨骼和关节 RACE c DNA文库。
短句来源
  人胚胎骨发生
    Objective:To explore the relationship between bone morphogenetic protein and development of human fetal bone.
    目的 :探讨骨形态发生蛋白与人胚胎骨发生及发育的关系。
短句来源
    Objective To explore the relationship between vascular endothelial growth factor(VEGF) and development of human fetal bone.
    目的探讨血管内皮生长因子(VEGF)与人胚胎骨发生及发育的关系。
短句来源
  “human fetal bone”译为未确定词的双语例句
    HLA expression in human fetal bone marrow mesenchymal stem cells
    人类胎儿骨髓间充质干细胞的白细胞抗原表达特征
短句来源
    Isolation and Identification of Mesenchyal Stem Cells Derived from Human Fetal Bone Maroow
    人胎儿骨髓间充质干细胞的分离及生物学鉴定
短句来源
    In Vitro Hepatic Differentiation of Mesenchymal Stem Cells from Human Fetal Bone Marrow
    胎儿骨髓间充质干细胞向肝细胞的诱导分化
短句来源
    Studies on Biological Characteristics of Mesenchymal Stem Cells Isolated from Human Fetal Bone Marrow
    人胎儿骨髓间充质干细胞的生物学特性研究
短句来源
    Identification of FIk1~+CD34~- pluripotent stem cells derived from human fetal bone marrow
    人胎儿来源Flk1~+CD34~-表型多能干细胞分离与鉴定(英文)
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  human fetal bone
The therapeutic effect of intravenous injection of human fetal bone marrow mesenchymal stem cells or summary culture of kidney cells were studied on models of chronic or acute renal failure in outbred albino rats.
      
Osteoclast generation from human fetal bone marrow in cocultures with murine fetal long bones
      
Pluripotent stem cells exhibiting similar characteristics can be isolated from human fetal bone marrow, heart, liver, muscle, lu
      
We then tested the effect of rhHAPO on the growth of human fetal bone marrow EPCs.
      
The capacity of the human fetal bone fragments to engraft into the SCID mice was evaluated.
      
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The effect of 3,5,3′-triiodothyronine (T_3) on the differentiation and proliferationof osteoblasts (human fetal bone-cells) cultured in serum-free medium was investi-gated by assays of alkaline phosphatase(ALP) activity, intracellular cyclic AMPlevel and collagen synthesis in the cell. The result suggested that T_3 in physiologicconcentration stimulated the differentiation of osteoblasts in vitro, and might beinvolved in bone formation in vivo.

本文利用无血清培养的人胎成骨细胞(OB)体外模型,通过对 OB 内碱性磷酸酶(ALP)活性、胶原合成、DNA、蛋白含量及 cAMP 水平的测定,研究了生理浓度的 T_3(3,5,3′-三碘甲腺原氨酸)对 OB 生长、功能、分化的作用,结果显示:生理浓度的 T_3可显著地增加 OB内 ALP 的活性,并有时间相关关系,T_3作用96小时可显著地增加~3H-脯氨酸掺入到胶原蛋白中;然而,在96小时时期内,却未显著地改变细胞内蛋白及 DNA 的含量。这些结果表明.T_3引起 ALP活性、胶原合成的增加,不是由于促进了 OB 的增生,而是直接促进了体外培养的 OB 的分化而增加其功能。本研究提示,T_3在生理浓度下与体内骨形成有关。

To study the biological characteristics of the human fetal bone marrow stromal cells, fetal bone marrow stromal cells (FBMSCs) and adult bone marrow stromal cells (ABMSCs)are cultured in vitro and their cell morphology, colony morphology, colony size are observed. The time of their clusters appearance, colonies formation, colonies confluence andspreading out completely are recorded. It is showed that there are no difference between theFBMSCs and ABMSCs in cell morphology, colony morphology,...

To study the biological characteristics of the human fetal bone marrow stromal cells, fetal bone marrow stromal cells (FBMSCs) and adult bone marrow stromal cells (ABMSCs)are cultured in vitro and their cell morphology, colony morphology, colony size are observed. The time of their clusters appearance, colonies formation, colonies confluence andspreading out completely are recorded. It is showed that there are no difference between theFBMSCs and ABMSCs in cell morphology, colony morphology, colony numbers and size ofCFU-F. The time of clusters appearance, colonies formation, colonies confluence, spreadingout completely of the FBMSCs is later than that of the ABMSCs. Our studies indicate thatFBMSCs is more primitive than ABMSCs and may have the better functions of supportinghaematopoiesis.

为了探讨人胚胎骨髓基质细胞的生物学特性,我们分别在体外培养人胚胎骨髓基质细胞(FBMSCs)和成人骨髓基质细胞(ABMSCs),在光镜下观察它们各自的细胞形态,集落形态和大小。结果表明:FBMSCs和ABMSCs的CFU-F细胞形态,集落形态,集落数,集落大小均无差异,但从出现小丛,形成集落,集落融合交错成片,到集落完全铺满瓶底,FBMSCs均晚于ABMSCs。表明FBMSCs较ABMSCs更原始幼稚,可能具有更好的支持造血功能。

Objective To study the differentiation of human mesenchymal stem cells (MSCs) derived from human fetal bone marrow and observe its telomerase activity. Methods MSCs were isolated from fetal bone marrow of the femur followed by cell culture and amplification. In situ hybridization was employed to detect telomerase activity in the MSCs. Subcutaneous implantation of MSCs into nude mice was performed to observe their differentiation potentials 4 weeks after the implantation. Results Human...

Objective To study the differentiation of human mesenchymal stem cells (MSCs) derived from human fetal bone marrow and observe its telomerase activity. Methods MSCs were isolated from fetal bone marrow of the femur followed by cell culture and amplification. In situ hybridization was employed to detect telomerase activity in the MSCs. Subcutaneous implantation of MSCs into nude mice was performed to observe their differentiation potentials 4 weeks after the implantation. Results Human MSCs were found positive for telomerase activity and they showed the potential to differentiate into such tissue cells as of the bone, cartilage, adipose, skeletal muscle, tendon-like tissue and unmyelinated nerve fiber-like bundles. Conclusion Human MSCs are multipotent stem cells that may differentiate into many types of tissue cells with high levels of telomerase activity.

目的 研究人骨瞩间充质干细胞(Mesenchymal stem cells,MSCs)的分化特性及其端粒酶活性。方法 取人胚胎股    骨骨瞩,分离、培养、扩增MSCs,原位杂交法检测MSCs的端粒酶活性。将MSCs种植于裸鼠皮下,4周后观察MSCs的    分化情况。结果人 MSCs呈端粒酶反应阳性。植入裸鼠体内后可分化成骨、软骨、脂肪、骨骼肌、肌腱样组织和无髓神经    纤维束样结构。结论人MSCs是一种具有多向分化能力的干细胞,具有较高的端粒酶活性。    

 
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