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asd gene
相关语句
  asd基因
     Cloning and sequencing of Shigella flexneri 2a asd gene
     痢疾福氏2a asd基因的克隆及其序列分析
短句来源
     On the basis of the flanking sequences of E. coli K 12 strain asd gene,a pair of primers were designed.
     本文根据大肠杆菌 (E .coli)K12asd基因两侧序列设计了一对引物 ,用全菌PCR扩增了福氏 2aT32株的asd基因及其两侧序列。
短句来源
     A host plasmid balancing system was established based on asd gene in a candidate vaccine strain(T32) of Shigella flexirie 2a.
     首先通过体内外重组的方法,构建了福氏2a痢疾菌T32asd基因缺陷的突变体FaD,作为抗原载体菌;
短句来源
     In this study, SL7207(asd-), the asd gene deletion mutant of the S. typhimurium strain (SL7207), was constructed by homologous recombination. The asd gene deletion mutant SL7207(asd-) was characterized by comparing its surviving and replicating both in vivo and in vitro with its parental strain SL7207.
     本研究通过同源重组的方式,构建了鼠伤寒沙门氏菌SL7207 asd基因突变株SL7207(asd-),并通过比较SL7207与SL7207(asd-)在体内、体外的生长、增殖情况,对SL7207(asd-)的部分生物学特性进行研究。
短句来源
     Methods:An asd gene_based host_plasmid balancing system was used to express CS6 and CTB in a genetic avirulent Salmonella typhi strain, in which CS6 and CTB can be expressed stably in the absence of antibiotic selection.
     方法 :以基因工程减毒的人伤寒沙门菌为抗原载体 ,利用基于asd基因功能互补的宿主染色体_质粒平衡致死表达系统 ,实现了在没有抗生素选择压力的条件下 ,CS6和CTB在人伤寒沙门菌中的稳定表达。
短句来源
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  “asd gene”译为未确定词的双语例句
     Asd gene was deleted in E. coli DH10B chromosome.
     coli DH10B:DH10B pGB_2Ωinv-hly Δasd、DH10B lacZ(?)
短句来源
     1. Cloning of crp and asd gene and construction of recombination suicide plasmidsThe crp and asd gene were amplified and cloned from C500 genome according to the
     2.接合转移与C500crp~-、C500crp~-/gfp~+、C500crp~-/asd~-、C500crp~-/gfp~+/asd~-、C500asd~-缺失株的筛选鉴定
短句来源
     Methods A recombinant attenuated Shigella flexneri 2a T32 deleted asd gene strain FWL01(pZCF16) carries a plasmid co expressing colonization factor antigen Ⅰ(CFA/Ⅰ) and coli surface antigen 6(CS6) of ETEC, two mixed recombinant strains FWL01(pZHY21) expressing CFA/Ⅰ and FWL01(pZLG6) expressing CS6 were compared and evaluated their immune responses.
     方法 以共表达肠毒素大肠杆菌定居因子CFA/Ⅰ和CS6的重组减毒福氏痢疾疫苗株FWL0 1(pZCF16 )和两种混合的表达CFA/Ⅰ的FWL0 1(pZHY2 1)和表达CS6的FWL0 1(pZLG6 )减毒福氏痢疾疫苗株 ,分别以同等剂量口服免疫BALB/c小鼠 ,比较二者肠毒素大肠杆菌定居因子的免疫效果。
短句来源
     A host\|plasmid balancing system composed with a Δ asd mutant (FaD) of an avirulent strain (T32) of Shigella flexneri 2a and plasmid harboring asd gene was used to express enterotoxigenic E.
     构建了携带asd、霍乱毒素B亚基 (CTB)基因的表达质粒 pYX2 0 1,与福氏 2a痢疾菌T32的△asd突变株FaD构成宿主 质粒平衡致死系统 ,用于在没有抗生素选择压力的情况下 ,稳定表达CTB抗原基因。
短句来源
     A gene fragment encoding for surface antigen CS6 of enterotoxigenic of Escherichia coli has been cloned into the plasmid pXL670, a new recombinant plasmid pSS64 was obtained by transforming E. coli X6097 with asd gene deletion. A safe and effective E.
     将编码肠毒素源性大肠杆菌定居因子抗原CS6 基因克隆至pXL670 ,转化asd 基因突变的E.coli X6097 ,获得重组质粒pSS64 ,再将后者转化至减毒的△aroA、△aroC、△asd 伤寒沙门氏菌,构建了无药物抗性且稳定的大肠杆菌和伤寒双价菌苗候选株。
短句来源
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  相似匹配句对
     Objective\ To obtain the asd gene of E.
     目的获得大肠杆菌全长asd基因。
短句来源
     Cloning and sequencing of the asd gene of Salmonella typhimurium
     鼠伤寒沙门氏菌asd基因的克隆及序列分析
短句来源
     On Gene Patenting
     基因的专利问题
短句来源
     -casein gene.
     -酪蛋白基因5'侧序列。
短句来源
     ASD, Galanz and dalmatian
     爱仕达、格兰仕与斑点狗
短句来源
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  asd gene
One clone, which carried the asd gene and expressed Asd activity, was isolated and chosen for further study.
      
The insertions had no adverse effect on expression of the downstream asd gene but resulted in leucine auxotrophy.
      
glutamicum asd gene complemented Escherichia coli asd mutants.
      
Therefore, we favor the interpretation that the asd gene is essential in mycobacteria.
      
Transformants with this ligation were screened for replacement ofthe central 1.6-kb PstI fragment that contains the asd gene.
      


The E. coli cs3 gene coding for CFA/11 antigen was site-specifically integrated into the asd gene locus of S. flexneri 2a vaccine strain T32, resulting in the asd gene inactivated. Meanwhile, the S. sonnei O antigen gene was cloned into a non-resistant expression vector pXL378 to construct the plasmid pXL390. By transforming the asd minus T32 strain with pXL390, the bivalent vaccine candidate strain FS01 against S. flexneri 2a and S. sonnei was finally obtained. Experiments showed that...

The E. coli cs3 gene coding for CFA/11 antigen was site-specifically integrated into the asd gene locus of S. flexneri 2a vaccine strain T32, resulting in the asd gene inactivated. Meanwhile, the S. sonnei O antigen gene was cloned into a non-resistant expression vector pXL378 to construct the plasmid pXL390. By transforming the asd minus T32 strain with pXL390, the bivalent vaccine candidate strain FS01 against S. flexneri 2a and S. sonnei was finally obtained. Experiments showed that the recombinant plasmid pXL390 was very stable in the asd minus T32 strain without the use of any antibiotics; FS01 strain was genetically stable and expressed two kinds of Shigella LPS-O antigens with no enhancement of its toxicity. Animal test demonstrated that FS01 strain, when administered subcutaneously in mice,could confer 100% protection against the intraperi-toneal challenges of virulent S. flexneri 2a and S. sonnei.

通过体内外基因重组,将大肠杆菌粘附因子cs3基因定位整合到痢疾杆菌福氏2a疫苗株T32菌染色体的asd基因内,使asd基因灭活;将来内O抗原基因克隆至无抗药性表达载体pXL378,获得重组质粒pXL390,将其转化asd-的T32受体菌,构建成福氏2a和宋内双价苗苗株FS01。实验表明:重组质粒pXL390在不带任何抗菌素基因的情况下,在asd-的T32受体菌内是稳定的。FS01株遗传稳定,能表达两种痢疾菌的PLS-O抗原,无明显毒性作用。动物试验表明,以FS01株皮下免疫的小鼠对福氏2a和宋内有毒株的腹腔攻击有100%的保护。

Objective\ To obtain the asd gene of E.coli by means of PCR.\ Methods\ DNA/RNA primer analysis software was used,the fragment of asd gene was amplified with long template PCR system and digested with restrictive enzymes. \ Results\ A pair of 21mer oligonucleotides were designed.\ The 1510 bp fragment of asd gene of E.coli was amplified by using these primers and identified with restrictive enzymes,the results showed...

Objective\ To obtain the asd gene of E.coli by means of PCR.\ Methods\ DNA/RNA primer analysis software was used,the fragment of asd gene was amplified with long template PCR system and digested with restrictive enzymes. \ Results\ A pair of 21mer oligonucleotides were designed.\ The 1510 bp fragment of asd gene of E.coli was amplified by using these primers and identified with restrictive enzymes,the results showed that the DNA fragments of asd gene were equal to those the “Gene Bank” shows.\ Conclusion\ The asd gene of E.coli has been obtained.\ The long template PCR system is suitable for amplification of long fragments.

目的获得大肠杆菌全长asd基因。方法采用计算机引物设计软件,长模板PCR扩增法及限制性内切酶酶切分析。结果设计一对E.coliasd基因PCR引物;经长模板PCR扩增获得1510bpPCR扩增片段;经限制性内切酶酶切分析,证明该片段与电脑查询的E.coliasd基因酶切谱相同。结论以本文设计的引物用长模板PCR扩增法得到的片段初步确认为E.coliasd基因。

A host plasmid balancing system was established based on asd gene in an avirulent strain of Salmonella typhi to express enterotoxigenic E.coli surface antigen CS3 and V.cholerae toxin subunit B(CTB). The plasmid can be stably maintained in the host and can express CS3 and CTB in the host cell without any antibiotic selection, although expression level and growth characteristics of the recombinant strain expressing either CS3 or CTB are superior to that of the recombinant strain...

A host plasmid balancing system was established based on asd gene in an avirulent strain of Salmonella typhi to express enterotoxigenic E.coli surface antigen CS3 and V.cholerae toxin subunit B(CTB). The plasmid can be stably maintained in the host and can express CS3 and CTB in the host cell without any antibiotic selection, although expression level and growth characteristics of the recombinant strain expressing either CS3 or CTB are superior to that of the recombinant strain which expresses both of the antigens. Antibo dies against CS3 and CTB can be detected in sera of mice immunized with recombinant bacteria either orally or subcutaneously, and mice immunized subcutaneously can be protected from challenging with virulent strain of Salmonella typhi. This work may be helpful in constructing multivalent recombinant vaccines for prevention of bacterial diarrhea.

通过体内外重组的方法, 构建了人伤寒菌流行株Ty2 的ΔaroA、ΔaroC 和asd- 基因缺失突变体(RS417), 作为抗原载体菌; 同时, 构建包含asd 基因的表达质粒pYX102 , 与RS417 一起, 构成宿主载体平衡致死系统, 用于在没有抗生素条件选择的情况下, 稳定表达克隆在表达质粒上的外源抗原基因。将肠毒素性大肠杆菌的菌毛抗原III(CS3) 基因和霍乱弧菌毒素B亚基(CTB) 基因分别克隆至pYX102 , 构建成相应的重组表达质粒,ELISA 检测结果证实菌毛抗原III和霍乱弧菌毒素B亚基在人伤寒菌RS417 中可以分别表达和共表达。重组菌免疫小鼠后可诱生相应的血清抗体, 虽然口服免疫和注射免疫产生的CS3 和CTB抗体效价有一定差别, 但皮下免疫组对人伤寒菌流行株Ty2 的攻击可提供完全保护。研究结果对细菌性腹泻疫苗的研制有参考价值。

 
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