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rapd group
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  “rapd group”译为未确定词的双语例句
     3. When all polymorphic RAPD bands were used for cluster analysis , 90 percent of common buckwheat were classified into one RAPD group and only several cultivars were classified into single group.
     3.基于RAPD标记的聚类分析表明,供试甜荞的遗传分化程度较小,约90%的品种归属于同一RAPD群,只有少数几个品种单独聚为一类。 其中,晋荞1号与其它品种的遗传距离最远。
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     group.
     对照组给予胃乃安口服,每次 4 粒,每日 3 次。
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     group.
     LPS+ICAM1单抗处理组;
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     The Standardization of RAPD
     RAPD技术的标准化问题
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     Methods:RAPD.
     方法:RAPD。
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  rapd group
The third RAPD group accommodated other isolates including the B morphotype isolate CWS43 from the United States.
      
The emergence of the RAPD group RC may have resulted from a combination of many factors.
      
RAPD group RC, which mainly contained CF isolates, in which the prevalence of nan1 was 82.5 %.
      
A number in parentheses beside a symbol distance within each RAPD group and beside arrows indicates distance between groups.
      


The relationship between virulence variation and RAPD fingerprint of 20 isolates of Verticillium dahliae from cotton were analyzed. The results showed that the 20 isolates were classified into 4 virulence groups based on the average severity index and reaction on 5 cotton cultivars. A total of the 92 RAPD bands were amplified using 10 random primers, and 51 of the 92 bands were found to be polymorphic. Relation between the virulence groups and RAPD groups obtained with 10 primers...

The relationship between virulence variation and RAPD fingerprint of 20 isolates of Verticillium dahliae from cotton were analyzed. The results showed that the 20 isolates were classified into 4 virulence groups based on the average severity index and reaction on 5 cotton cultivars. A total of the 92 RAPD bands were amplified using 10 random primers, and 51 of the 92 bands were found to be polymorphic. Relation between the virulence groups and RAPD groups obtained with 10 primers is low. However, virulence groups related closely with some RAPD patterns generated by single primer. The similar RAPD patterns of different isolates of VG1,VG2 and VG4 were amplified using OPC15 primer respectively, and similar results were generated using other primers(OPC05, OPM13,OPA02,OPL02).

对20个大丽轮枝菌菌株的致病性进行了测定和RAPD指纹扩增,完成了供试菌株致病类群与其RAPD指纹组间的相关性分析。结果表明,根据20个菌株对5个棉花品种的抗感反应及其平均病情指数分为4种不同的致病类群;用10个随机引物对20个菌株进行RAPD-PCR扩增,产生92条DNA带(其中51条为多态带);用计算机进行聚类分析,20个供试菌株聚类为9个RAPD指纹组。相关性分析表明,致病类群与RAPD指纹组无明显相关性;但与部分单引物扩增的RAPD谱型有明显对应关系,如引物OPC-15随机扩增的RAPD谱型,除致病类群VG3的菌株外,其余致病类群均有对应的RAPD指纹谱带;其它引物(OPC-05,OPM-13,OPA-02,OPL-02)也有相似的结果。

Forty eight isolates of Verticillium dahaliae have been collected and isolated from same cotton field in March, 1994 and March, 1995 respectively. These isolates were analyzed using RAPD fingerprint technique. The results showed that a total of 120 reproducible RAPD bands were obtained on 48 isolates with 17 random primers selected from 260 primers. Forty three of the 120 bands ( 35.83%) were polymorphic. When RAPD bands were used for cluster analysis using the...

Forty eight isolates of Verticillium dahaliae have been collected and isolated from same cotton field in March, 1994 and March, 1995 respectively. These isolates were analyzed using RAPD fingerprint technique. The results showed that a total of 120 reproducible RAPD bands were obtained on 48 isolates with 17 random primers selected from 260 primers. Forty three of the 120 bands ( 35.83%) were polymorphic. When RAPD bands were used for cluster analysis using the PHYLIP 3.5 c UPGAME program, all isolates were clustered into 4 RAPD groups, and the genetic difference of RG4 containing 5 isolates of 1995 are very different from others.

分别于1994年3月和1995年3月从同一棉田,采集分离了48个大丽轮枝菌菌株.应用RAPD技术,从240个随机引物中筛选了17个扩增效果好的引物,对供试菌株进行了DNA指纹分析.结果表明:17个引物对48个菌株扩增出120条DNA分子片段,其中43条分子片段为多态带,占35.83%.将扩增结果输入计算机,用PHYLIP分析软件(3.5版本),UPGAME程序进行类聚分析,结果表明95年的5个菌株与其它菌株存在明显的遗传差异

RAPD was used to analyze the genetic diversity and virulence phenotypes of Xanthomonas oryzae pv. oryzicola , the bacterial leaf streak pathogen of rice in China. Twenty of 60 used random primers produced consistent and clear banding patterns that generated a set of products from 0.5 kb to 3.5 kb in size. Cluster analysis was conducted using UPGMA. The result showed that genetic variation existed obviously among isolates of rice bacterial leaf streak (BLS) from different regions,isolates tested...

RAPD was used to analyze the genetic diversity and virulence phenotypes of Xanthomonas oryzae pv. oryzicola , the bacterial leaf streak pathogen of rice in China. Twenty of 60 used random primers produced consistent and clear banding patterns that generated a set of products from 0.5 kb to 3.5 kb in size. Cluster analysis was conducted using UPGMA. The result showed that genetic variation existed obviously among isolates of rice bacterial leaf streak (BLS) from different regions,isolates tested could be divided into 7 lineages (groups) .Lineages 1,2 and 5 were the most frequently detected,including 7,8 and 6 strains respectively. Thirteen pathotypes(virulence phenotypes) of X.oryzae pv. oryzicola were identified according to resistant and susceptible react of 12 genotypes to the strains using 12 rice near iso lines, each containing a single gene for resistance.The 13 pathotypes grouped into six clusters. There was a weak correlation between RAPD groups and virulence phenotypes.

利用 6 0个随机引物对来自我国不同稻区的水稻细菌性条斑病菌株基因组DNA进行PCR扩增 ,其中2 0个引物均出现清晰和重复性的DNA扩增片段 ,扩增片段大小在 0 .5kb至 3.5kb ;应用Statistics软件UPGMA程序进行树状聚类分析 ,聚类结果表明 ,我国水稻细菌性条斑病菌具有明显的遗传分化 ,在遗传距离为 0 .30时 ,供试菌株可划分为 7个遗传相似组 (谱系 )。其中第 1、第 2和第 5组为优势组群 ,分别由 7、8、6个菌株组成。根据菌株在 12个已知基因品种上的抗感反应 ,将供试菌株分为 13个致病型 ,在遗传距离为 0 .5 0水平上聚类归属于 6个簇。菌株的致病类群与其DNA的遗传变异具有弱相关性

 
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