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gm-maize
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  相似匹配句对
     The Difficulty of GM
     定义术语:"遗传修饰GM"的困难
短句来源
     GM AGRICULTURE AND ITS SD
     转基因农业与可持续发展
短句来源
     A Proof of the AM-GM Inequality
     AM-GM不等式的一个证明
短句来源
     gentamicin and L-arginine (GM and L-Arg);
     庆大霉素加左旋精氨酸 (GM加L -Arg)组 ;
短句来源
     and maize cross.
     与玉米杂交导入一个小麦加倍单倍体 (DH)植株中。
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  gm-maize
Statistical analysis of farm questionnaires to search for differences between GM- and non-GM-maize
      
Two different detection systems were applied: one based on the 35S promoter fragment which is present in GM-soybeans and in GM-maize and one based on the nos terminator sequence which is present only in GM-soybeans.
      


Nucleotide\|based amplification method is an important system for the identification of genomic modified foods (GMF). Roundup Ready Soybeans (Monsanto company), Bt 176 GM maize (Novartis/Ciba\|Geigy company) and Cecropin D capsicum was used as material to search for the feasibility of investigating the safety of GMF by PCR method. Primers specific for inserted genes and crop endogenous genes in Roundup Ready Soybeans, Bt 176 maize and Cecropin D capsicum were applied. The discrimination system for GM soybeans,...

Nucleotide\|based amplification method is an important system for the identification of genomic modified foods (GMF). Roundup Ready Soybeans (Monsanto company), Bt 176 GM maize (Novartis/Ciba\|Geigy company) and Cecropin D capsicum was used as material to search for the feasibility of investigating the safety of GMF by PCR method. Primers specific for inserted genes and crop endogenous genes in Roundup Ready Soybeans, Bt 176 maize and Cecropin D capsicum were applied. The discrimination system for GM soybeans, GM maize and GM capsicum from the counterpart of non\|GM products and the detection system for correlating marker genes and transgenes are established. The method was easy and fast, and the corresponding results fixed the standard or declared data.

以国际市场上转基因食品的主流产品转基因大豆及玉米和我国生产的转基因辣椒为材料 ,以PCR方法为基础 ,研究适用于转基因食品安全性检验的核酸检测技术。针对抗除草剂 (孟山都公司 )GM 大豆 ,转Bt 176玉米 (Novartis Ciba Geigy公司 )及转抗菌肽辣椒 (华农 )产品的插入基因及调控序列设计不同引物进行PCR检测。建立了转基因大豆、玉米、辣椒的鉴别和相关标记基因、目的基因检测的技术 ,该方法简便快速 ,检测结果与标准及申报材料相符。

Study the method of quantitative/identified detection of genetically modified (GM) maize Mon810 components infoods and in feeds by real-time PCR. The primer and probe set overlapping the junction was designed and used for assay. Theresults demonstrated this method is useful for identifying the event-specific GM maize Mon810. Some samples were tested notonly the GM maize Mon810 but also another GMO components.

采用实时荧光PCR技术,建立了定量(性)鉴定检测加工产品中转基因玉米Mon810成分的方法。实验设计的可以扩增玉米自身基因和外源基因边界序列的引物和探针具有品种和品系特异性,特异性地检测出食品、饲料等加工产品中转基因玉米Mon810成分。某些检测样品不仅检出转基因玉米Mon810成分,还同时检出其它转基因玉米品系或其它转基因品种。本研究实验建立的转基因玉米Mon810品系鉴定检测方法,即可以用于加工产品中转基因成分的定量检测(检测低限为0.1%),也可以用于定性检测,或作为常规PCR定性检测后的确证实验方法。

To detect and identify six lines of genetically modified (GM) maize, polymerase chain reaction (PCR) assays were performed in this study. Primers specific for inserted genes in the Event 176 GM maize (Novartis company), Bt11 (Novartis company), Mon810 (Monsanto company), T14/T25 (AgrEvo company), CBH-351 (AgrEvo company) and GA21 (Monsanto company) were used to conduct the PCR assays. PCR method was established to detect and identify lines of GM maize.

以PCR方法鉴定检测六种商业化种植的基因改良玉米 (geneticallymodifiedmaize,简称GM 玉米 )。针对Mon810 (Monsanto公司 )、Bt11(Novartis公司 )、Event176 (Novartis公司 )、CBH 35 1(AgrEvo公司 )、T14/T2 5Liberty (AgrEvo公司 )及GA2 1(Monsanto公司 )GM 玉米转入的外源基因质粒图谱 ,设计具有品系特异性的引物进行PCR检测 ,建立了GM 玉米品系鉴定检测的方法。

 
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