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amplification pattern
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  “amplification pattern”译为未确定词的双语例句
     The PCR amplification pattern of 207rice isolates from Fujian province strongly correlative to the results obtained byusing GUYI 1 and KA3. The fertile isolate 81278ZB15, which belongs to adominant virulence type in the province, has differentrep-PCR DNA fingerprints and virulence spectrum results suggested thatgenetic polymorphic and virulence spectrum of 81 278ZB 15 were greatlydifferent than GUY11. 90 ascospores of the cross 81278ZB15 and GUY11 wererandomly isolated.
     毒性测定和rep-PCR DNA指纹分析结果表明,81278ZB15和GUY11二个菌株的毒性谱和遗传多态性差异很大。 用其作亲本杂交,建立一个随机的有性后代群体,并对其进行了交配型、无毒基因的遗传分析。
短句来源
     To confirm its application in mating type assessment, 10 tester isolates were tested by PCR. The PCR amplification pattern corresponded to their known mating type.
     以10个已知交配型的标准菌株基因组DNA为模板,结果PCR测得的交配型与其已知的交配型一致。
短句来源
     Moreover, there are no variations among these four hybrids in the PCR amplification pattern and the restriction pattern, indicating that mitochondrial genes are highly conserved in white poplars.
     4种杂交组合线粒体基因片段的PCR扩增与酶切图谱完全一致,未发现多态性,表明白杨线粒体基因高度保守.
短句来源
     Steadily and realibly profile was obtained in the 33 genotypes belonging to 7 species using the optimal system and amplification program. The size of fragments obtained ranged from 120~1500 bp, the total bands and amplification pattern were distinctive in different species and cultivals.
     该优化体系在7种柿属植物33个基因型的I-RAP分析中获得较理想的扩增结果,扩增片段120~1500bp,不同种和柿种以下不同品种间扩增条带总数和带谱相同。
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  相似匹配句对
     pattern
     格调之美
短句来源
     Pattern and Culture
     作用模式与文化问题
短句来源
     PCR amplification;
     普通PCR扩增目的片段;
短句来源
     The method of amplification in E-C and C-E translation
     论英汉互译中的增补翻译
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  amplification pattern
This simulation provided interesting results that displayed a complex amplification pattern in a rich spectrum of frequencies and locations.
      
The similarity coefficient values based on the amplification pattern support an equidistant position of the three test species.
      
The amplification pattern of orfB is different in the CMS cauliflower and its maintainer line NKC-B.
      
odemensis shared the same amplification pattern of a single sized NTS region.
      
Three isolates which showed a low level of pathogenicity on all carnation cultivars tested shared an identical amplification pattern and are probably saprophytic F.
      
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A new economic and efficient DNA polymorphism assay was developed in 1990 that is based on the amplification by polymerase chain reaction (PCR) of random DNA segments using primers of arbitrary nucleotide sequence. Authors have now adapted this type of amplification to rice mitochondrial genome. Using 6 rice varieties in conjunction with 7 of 20—27 mer oligonucleotide primers, the AP PCR products revealed that the amplified DNA bands fell into two categories, the evolutively conserved the cytoplasmic...

A new economic and efficient DNA polymorphism assay was developed in 1990 that is based on the amplification by polymerase chain reaction (PCR) of random DNA segments using primers of arbitrary nucleotide sequence. Authors have now adapted this type of amplification to rice mitochondrial genome. Using 6 rice varieties in conjunction with 7 of 20—27 mer oligonucleotide primers, the AP PCR products revealed that the amplified DNA bands fell into two categories, the evolutively conserved the cytoplasmic specific. It is suggested that AP PCR assay of mtDNA may help to classfy or identify the cytoplasms in rice. By comparing“fingerprints”among the WA type Cytoplasmic male sterility (CMS) rice, its Maintainer and Restorer lines, as well as its hybrid, one CMS cytoplasm specific band (primer R 2/630 bp) and one normal cytoplasm specific segment (primer V 5/707 bp) could be directly identified among the set of amplified DNA fragments. Further, some difference in the amplification patterns of mtDNA between CMS line and its hybrid, which infers that rearrangement of mitochondrial genome in hybrid rice probably happened.

为了研究水稻(Oryza sativa L.)细胞质雄性不育(CMS)与线粒体基因组的关系,应用AP-PCR 分析,用7 个任意单引物对6 种水稻品系线粒体DNA 进行了扩增。水稻线粒体DNA 的AP-PCR 产物可分为三种类型:(1)所有供试品系均能扩增的片段,它们代表了线粒体DNA 在进化上的保守性序列。有4 个引物检测到这类片段。(2)2 个以上水稻品系共同出现而在全部供试材料间存在差异的扩增片段,这类片段是检测水稻线粒体DNA多态性的主要来源。(3)一种细胞质类型所特有的扩增片段,从引物R2 和V5 的扩增产物中发现了这类片段,它们可能与CMS有关联。另外,WA型不育系珍汕97A 与其杂种之间在6 个引物的扩增图谱上均存在不同程度的差异,说明两者的线粒体DNA序列结构可能存在某种差别

wo Chinese wax gourd (Benincasa hispida Cogn)cultivars and one chiehqua(B.hispida Cogn.var.chieh-qua How) Cultivar were examined for random amplifiedpolymorphic DNA(RAPD)genetic markers with twenty 10-mer random primers. Of the 20primers screened; 1 did not have any amplification;7amplified only monomorphic DNApatterns;and 12 gave reproducible; polymorphic DNA amplification patterns. The DNA bandpatterns generated were prinlcr-and genotype-dependent. 9 primers produced unique bandingpatterns for...

wo Chinese wax gourd (Benincasa hispida Cogn)cultivars and one chiehqua(B.hispida Cogn.var.chieh-qua How) Cultivar were examined for random amplifiedpolymorphic DNA(RAPD)genetic markers with twenty 10-mer random primers. Of the 20primers screened; 1 did not have any amplification;7amplified only monomorphic DNApatterns;and 12 gave reproducible; polymorphic DNA amplification patterns. The DNA bandpatterns generated were prinlcr-and genotype-dependent. 9 primers produced unique bandingpatterns for each of the two Chinese wax gourd cultivars;6 primers for Chinese wax gourdvar.without wax and chieh-qua and 12 primers for Chinese wax gourd var. with wax andchieh-qua.Only 3 primers gave unique banding patterns for each of the 3 cultivars.The threecultivars were very similar when comparison was made on the basis of RAPD patterns.

利用随机扩增多态性DNA(RAPD)技术分析了2个冬瓜品种和1个节瓜品种。所用的20个引物有19个能扩增出DNA谱带。其中,7个引物对3个品种扩增的DNA谱带没有多态性;12个引物能产生具有重现性的DNA多态性谱带.有9个引物对2个冬瓜品种的DNA扩增具有多态性,能分别鉴别出2个冬瓜品种;6个引物对青皮冬瓜和江心节瓜的DNA扩增分别具有多态性;有3个引物对3个品种的DNA扩增分别具有多态性,能够一次鉴别出3个品种。根据3个品种间相似系数比较,3个品种间相似程度很高.

Two methods of DNA extraction from Gracilaria lemaneiformis were compared. The integrity of chromosomal DNA molecules was good for both of the methods. Samples from CTAB method were better than that from proteinase K method in DNA production and protein content. Conditions of RAPD PCR were optimized in the present paper: RAPD reaction in 25μL reaction volumes (containing 2.5m mol/L of Mg 2+ ,100μ mol/L of dNTP, 25ng of total DNAs,0.2μ mol/L of primer and 1 unit of Tag DNA polymerase) was performed smoothly...

Two methods of DNA extraction from Gracilaria lemaneiformis were compared. The integrity of chromosomal DNA molecules was good for both of the methods. Samples from CTAB method were better than that from proteinase K method in DNA production and protein content. Conditions of RAPD PCR were optimized in the present paper: RAPD reaction in 25μL reaction volumes (containing 2.5m mol/L of Mg 2+ ,100μ mol/L of dNTP, 25ng of total DNAs,0.2μ mol/L of primer and 1 unit of Tag DNA polymerase) was performed smoothly with 35 cycles of denature 1 min at 94℃, annealing 1 min at 35℃ and elongation 2 min at 72℃, and stable RAPD amplification patterns were obtained.

比较两种从龙须菜中提取总DNA的方法,这两种方法得到的染色体DNA具有较好的完整性,从DNA的产量、蛋白质含量等指标可以看出,CTAB法优于蛋白酶K法。实验对龙须菜RAPD反应条件进行了优化:在25μL反应体系中,含有Mg2+2.5mmol/L,dNTP100μmol/L,总DNA25ng,引物0.2μmol/L和Taq酶1U,经94℃变性1min,35℃退火1min,72℃延伸2min,35个循环,得到稳定的RAPD扩增图谱。

 
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