full genome 全基因(2)   全基因      Sequencing the Full Genome, Cloning and Expressing of Structural Protein and the Related Research in SARS-CoV      SARS冠状病毒全基因测定、结构蛋白基因克隆、表达及相关研究 短句来源      Two recombinant vaccinia virus(RV-1 and RV-2)strains containing a full genome of polio virus(PV)type Ⅰ and encoding structural protein Vpl 2.5 Kd cDNA of PV have successfully expressed the antigenic protein of PV type Ⅰ.      含脊髓灰质炎病毒1型(简称 PV1)全基因7.4Kb cDNA 和编码 PV1外壳蛋白 VP12.5Kb 的 cDNA 片段的两株重组痘苗病毒(RV-1和 RV-2)表达了 PV1抗原。 短句来源   “full genome”译为未确定词的双语例句      The sequence of the full genome of the PCV-2 GD1 strain was compared with the other PCV isolates in GenBank,indicating that the gene sequence of this PCV-2 isolates was closey related with a Germany isolate AF 20 1897,with 99.9 % homology,and having 94.4 %～99.7 %nucleotide identity with the other PCV-2 isolates.      通过BLAST对所测GD1株核苷核酸序列早国内外已登录GenBank中的PCV_2病毒核苷酸序更进行同源性比较 ,与PCV_2参考毒株的核苷酸同源性介于 94 4 %～ 99 7%之间 ,与德国株PCV_2 (AF2 0 1897)的同源性最高 ,为 99 7% ; 短句来源      Utilizing the RTPCR,nested and halfnested PCR techniques,7 overlaping cDNA fragments covering the full genome of Cstrain were amplified from total RNA extracted from spleens of experimentally infected rabbits. These fragments were sequenced and spliced into fulllength cDNA after being cloned into pMD18T or pGEMT Easy vectors.      应用RT PCR、NestedPCR和Half nestedPCR技术从实验感染兔脾组织的总RNA中得到了覆盖猪瘟病毒(CSFV)C 株全基因组的7个cDNA重叠片段,分别克隆于pMD18 T或pGEM TEasy载体后进行测序。 短句来源      By RACE,2 overlapping cDNA fragments(3′PCR and 5′PCR fragments) covering the full genome of swine vesicular disease virus strain HK′1/70 were amplified from total RNA extracted from experimentally infected suckling mice. These fragments were cloned into pGEM-T Easy vector,respectively.      从实验感染猪水泡病病毒(SVDV)的乳鼠组织中提取RNA,利用长距离的RACE技术,扩增出覆盖SVDVHK′1/70株全基因组的2个忠实性的cDNA重叠片段(3′PCR片段和5′PCR片段),分别克隆进pGEM-T Easy载体。 短句来源      Chapter two reported by RT-PCR, we get the HCV full genome by fragments from the patient who infected with the HCV in Hubei province.      第二章描述了采用分段RT-PCR的方法从湖北省HCV 感染阳性病人血清中克隆得到了HCV 的全基因组,通过软件对其进行基因型和进化树的分析。 短句来源      According to the conservative sequence of TuMV different isolates, a set of primers was desinged to amplify the 9. 4kb cDNA of TuMV isolate CHN5 covering about 94% of its full genome by immunocapture RT-PCR, and cloned the cDNA into a T-vector.      根据芜菁花叶病毒各株系保守序列，设计引物，利用免疫捕获RT-PCR的方法获得芜菁花叶病毒CHN5株系覆盖其全长基因组94％的长约9.4Kb的DNA片段，并克隆到T-载体中去。 短句来源 更多          相似匹配句对      genome.      基因组。 短句来源      1757 full viral genome sequences are available in GenBank.      至今，已经测定全基因组序列的病毒种类达1757种。 短句来源      GENOME ANALYSIS      苇状羊茅的染色体组分析 短句来源      Cloning of the Full-length cDNA of HCLV Genome and Phylogenetic Analysis      猪瘟病毒兔化弱毒(HCLV)疫苗株基因组全长cDNA的克隆与序列分析 短句来源      Full of sentiment      让生活充满情趣的厨房用品 短句来源 查询“full genome”译词为用户自定义的双语例句     我想查看译文中含有：的双语例句 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。   full genome The full genome sequence of this latter isolate is unique inasmuch as it is nearly identical to that of PVYN isolates for the first 2,000 nucleotides (nts), after which it very strongly resembles PVYNA-NTN isolates for the next 600 nts. 例句来源       Here we describe the full genome sequences of five new PanSV isolates (including two new strains) sampled from southern and western Africa, and report that PanSV and MSV do indeed have similar patterns of diversity. 例句来源       Only two PanSV full genome sequences have ever been reported: one from Kenya [2], and the other from South Africa [17]. 例句来源       Here we present annotated full genome sequences of four new SISV isolates, including a new strain of both SSRV and SSV, and one potentially new SISV species, sampled from wild grasses in La Réunion and Zimbabwe. 例句来源       The full genome of BPeMV consists of 6375 nucleotides. 例句来源       更多                    Two recombinant vaccinia virus(RV-1 and RV-2)strains containing a full genome of polio virus(PV)type Ⅰ and encoding structural protein Vpl 2.5 Kd cDNA of PV have successfully expressed the antigenic protein of PV type Ⅰ.The antigen was located in the infected Hep-2 cell with an appearence specific granular particles by IF assay.The expressed PV proteins showed as four bands in RV-1 and two bands in RV-2 on Western Blot.They reacted well with anti-PV McAb.The animals immunlized with the two recom...             含脊髓灰质炎病毒1型(简称 PV1)全基因7.4Kb cDNA 和编码 PV1外壳蛋白 VP12.5Kb 的 cDNA 片段的两株重组痘苗病毒(RV-1和 RV-2)表达了 PV1抗原。经荧光标记的抗 PV1型特异性单克隆抗体检测,感染了 RV-1和 RV-2的 Herp-2细胞呈阳性荧光反应。免疫印迹实验证实两株重组痘苗病毒分别表达 PV1抗原三条和两条特异性蛋白带。免疫家兔后能诱导产生抗 PV1中和抗体。本文对重组病毒表达 PV1抗原的效率进行了初步讨论。 文摘来源          Rice dwarf virus full genome 12 gene segments were cloned and 25000 nucleotides were sequenced.The gene functions of each segment were analyzed according to amino acid sequences and experimental data.The coding regions of segments S6,S9,S10 and S11 encoding nonstractural proteins were cloned into expression vector pTricHisA,pBV221,pTric HisB,pBV221,respectively.The expressed products were analyzed by SDS PAGE and further confirmed by Western blotting.Using the E.coli expression products,th...             根据本实验室对水稻矮缩病毒（ＲｉｃｅＤｗａｒｆＶｉｒｕｓ，ＲＤＶ）中国福建分离株基因组全序列的测定，分析了其编码蛋白的功能，并将ＲＤＶＳ６，Ｓ９，Ｓ１０，Ｓ１１编码区ｃＤＮＡ分别克隆到表达载体ｐＴｒｃＨｉｓＡ，ｐＢＶ２２１，ｐＴｒｃＨｉｓＢ和ｐＢＶ２２１中，构建成表达质粒ｐＴＨ－Ｓ６，ｐＢＶＰ９，ｐＴＨ－Ｓ１０，ｐＢ－ＶＰ１１。分别用ＩＰＴＧ或温度诱导大肠杆菌ＤＨ５α，经ＳＤＳ－ＰＡＧＥ及相应抗体的Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ检测证明Ｓ６，Ｓ９，Ｓ１０，Ｓ１１编码蛋白在大肠杆菌中获得成功表达。另外，还将ＲＤＶ外层外壳蛋白基因Ｓ８、微核心蛋白基因Ｓ７及编码非结构蛋白基因Ｓ９通过基因枪导入到水稻植株中，抗病性正在研究之中。 文摘来源          The studies on insect cytochrome P450 are progressing and some interesting findings are summarized. The total number of P450 genes in Drosophila melanogster and Anopheles gambiae was described as 90 and 111 respectively due to the availability of full genome sequences. The DDT resistance of D. melangoster was found to be associated with the over transcription of P450 Cyp 6g1 Two P450 genes( Cyp315a1, Cyp302a1) in the ecdysteroid synthesis pathway were identified. The abi...             报道了有关细胞色素P45 0研究的一些新发现。果蝇和冈比亚按蚊基因组测序的完成 ,使人类对昆虫P45 0的多样性有一完整的概念 ,已查明果蝇和冈比亚按蚊基因组中分别含有 90种和 1 1 1种P45 0基因。P45 0介导的果蝇对DDT的抗性被证明是Cyp6g1基因超量表达的结果。昆虫可以窃听植物分子信号 (水杨酸、茉莉酮酸 ) ,通过P45 0的诱导机制增强自身对植物防御物质的反防御能力。从分子水平上鉴定了 2个参与蜕皮素合成的线粒体P45 0基因。细胞色素P45 0在昆虫信息素降解中的作用得到鉴定。 文摘来源   << 更多相关文摘         相关查询: full-genome full length genome full genome of hcv full length hcv genome full-length genome genome full full-length genome clone post genome genome organization full preparation genome engineering  对查询结果不满意

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