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   glycoprotein   gene 的翻译结果: 查询用时:0.127秒
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glycoprotein   gene
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     P-glycoprotein and intractable epilepsy
     P-糖蛋白与难治性癫痫的关系
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     conclusion  ①.
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     The gene of the glycoprotein g13 of EHV- I was sequenced.
     本文分析了其膜糖蛋白g13基因的碱基序列,并由此推导其相应的氨基酸序列。
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Two recombinants of vaccinia virus ( Tian Tan strain ) containing the coding sequence of Challenge Virus Standard ( CVS ) rabiesvirus glycopro tein were produced by insertion of the glycoprotein gene into the vaccinia tk gene. The coding sequence was controled under the vaccinia early -late promoter p7.5. The recombinants differed slightly in its sequence upstream of the translation start codon and both express effectively mature glycoprotein of rabiesvirus, which was transported to the...

Two recombinants of vaccinia virus ( Tian Tan strain ) containing the coding sequence of Challenge Virus Standard ( CVS ) rabiesvirus glycopro tein were produced by insertion of the glycoprotein gene into the vaccinia tk gene. The coding sequence was controled under the vaccinia early -late promoter p7.5. The recombinants differed slightly in its sequence upstream of the translation start codon and both express effectively mature glycoprotein of rabiesvirus, which was transported to the cell membrane.Immunological assays and trial immunizations in animals showed that the molecular weight of non-fusion glycoprotein of rabiesvirus approximates 64kD. It react specifically with antibody against authentic glycoprotein of rabiesvirus. Each recombinant induced rabiesvirus neutralizing antibodies in mice and rabbits and protection against lethal street virus challenge.

把狂犬病毒标准攻击株(Standard challenge virus)的糖蛋白编码序列插入天坛株痘苗病毒的胸腺核苷激酶(TK)基因区,并置于痘苗病毒7.5k蛋白启动子(P7.5)的控制之下,构建了两株重组痘苗病毒。它们在起始密码子上游一侧的核苷酸序列稍有差别,但均能有效地表达狂犬病毒的成熟糖蛋白并能转运至细胞膜上。免疫学试验及动物试验结果表明,重组痘苗病毒表达的狂犬病毒非融合性糖蛋白分子量约为64kD;均能与狂犬病毒天然糖蛋白单克隆抗体发生特异性反应,并能在小鼠和家兔中诱生高滴度狂犬病毒中和抗体(VNA)并对致死量狂犬病毒攻击动物具有保护能力。

Two strains of recombinant vaccinia virus expressing rabies glycoprotein gene were inoculated into dogs by three ways. High tilers of neutralizing antibodies were induced by scarification and intramuscular routes, but there was no seroconversion when being immunized by oral route Immunologic effects were also observed when the recombinants were inoculated into mice by various ways.

用表达狂犬病毒糖蛋白的两株重组痘苗病毒对狗口服、划痕和肌肉注射三种途径的免疫效果进行观察。结果口服免疫未见抗体阳转,而划痕和肌注免疫在第七天就能诱导中和抗体。中和抗体滴度达642。以肌肉注射、皮下注射和脚掌注射免疫小鼠,亦获得较好的免疫效果。

Recombinant vaccinia virus carrying rabies glycoprotein gene (RTTVV-3) was passaged on chicken embryo cells, Blue plaque were present on agarose containg neutral red and X-gal. The virus titre was 3×10~7~4×10~7PFU/mL between 11th to 30th passage. A similar HI titer was found with the virus of differential passage. The molecular weight was 64 KD tested by Western bloting. The immunogenicity and protect effect of recombinant in mice were invariable affter different passage, The results above showed...

Recombinant vaccinia virus carrying rabies glycoprotein gene (RTTVV-3) was passaged on chicken embryo cells, Blue plaque were present on agarose containg neutral red and X-gal. The virus titre was 3×10~7~4×10~7PFU/mL between 11th to 30th passage. A similar HI titer was found with the virus of differential passage. The molecular weight was 64 KD tested by Western bloting. The immunogenicity and protect effect of recombinant in mice were invariable affter different passage, The results above showed that the biological charactreistics.

将重组病毒在原代鸡胚细胞上连续传至30代(P30),其结果P30与第11代(P11)一样,在含中性红和X-gal的琼脂糖平板上为蓝色蚀斑;空斑法测定P11至P30,各代病毒滴度均稳定在3×10~7PFU/mL~4×10~7PFU/mL;血凝试验表明。不同代次的血凝滴度基本稳定在相同水平;Western blot检测P30与P11在同一位置出现相同蛋白带;血清中和试验证实传代后的重组病毒抗原活性及保护性稳定。重组病毒经传30代后,在生物学特性和表达水平上都具有良好的遗传稳定性。

 
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