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guanosine- -monophosphate gmp
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     Rotational Barrier of Guanosine Nucleoside about Z-DNA
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     Hydrolysis of Aderosine Monophosphate and Guanosine Monophosphate by Lanthanides
     稀土元素对腺嘌呤及鸟嘌呤单核苷酸的水解断裂作用
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  guanosine- -monophosphate gmp
Guanosine-5'-monophosphate (GMP) was evaluated as a neuroprotective agent against the damage induced by glutamate in rat hippocampal slices submitted to glucose deprivation.
      
Furthermore chlorpromazine was irradiated in the presence of guanosine-5-monophosphate (GMP) in pH 7 buffered solution, leading to a new photoproduct.
      
Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8.; HPRT) catalyzes the salvage synthesis of inosine-5'-monophosphate (IMP) and guanosine-5'-monophosphate (GMP) from the purine bases hypoxanthine and guanine, respectively.
      


The Interaction between Tb~(3+) and DNA was studied.Based on the fluorescence spectrum of Tb~(3+),it can be concluded that DNA can enhance the fluorescence intensity of Tb~(3+),and the interaction between Tb~(3+) and thermal denatured DNA is more effective than DNA.We studied the interaction mechanisms of Tb~(3+) and seven kinds of nucleotide,which are deoxyadenosine monophosphate(d-AMP)、deoxyguanosine monophosphate(d-GMP)、deoxycytidine monophosphate(d-CMP) deoxythymidine triphosphate(d-TTP)、guanosine...

The Interaction between Tb~(3+) and DNA was studied.Based on the fluorescence spectrum of Tb~(3+),it can be concluded that DNA can enhance the fluorescence intensity of Tb~(3+),and the interaction between Tb~(3+) and thermal denatured DNA is more effective than DNA.We studied the interaction mechanisms of Tb~(3+) and seven kinds of nucleotide,which are deoxyadenosine monophosphate(d-AMP)、deoxyguanosine monophosphate(d-GMP)、deoxycytidine monophosphate(d-CMP) deoxythymidine triphosphate(d-TTP)、guanosine monophosphate(GMP)、guanosine diphosphate(GDP) and guanosine triphosphate(GTP).The experiment results showed that the interaction between Tb~(3+) and nucleotides which have Guanine is notable,and the others do not react with Tb~(3+).We also studied the interaction between Tb~(3+) and four kinds of nucleoside,including deoxyadenosine(dA),deoxyguanosine(dG),deoxycytidine(dC) and deoxythymidine(dT).The experiment results showed that the four kinds nucleoside don't react with Tb~(3+).So the fluorescence enhancement to Tb~(3+) may be also related to phosphate groups.In order to make this conclusion more clearly,the interaction of Tb~(3+) with dG、GMP、GDP and GTP were studied.It showed that dG which doesn't have phosphate group can not react with Tb~(3+).But the three kinds of nucleotide GMP、GDP、GTP,which have one、two and three phosphate groups respectively react with Tb~(3+),and the order of fluorescence intensity enhancement is:GTP>GDP>GMP.It showed that phosphate groups participate the reaction between DNA and Tb~(3+).So we can concluded that DNA enhancing the fluorescence intensity of Tb~(3+) is the results of the synchronous effect by Guanine and phosphates.

研究了脱氧核糖核酸(DNA)与Tb3+的相互作用,发现DNA能增敏Tb3+的特征荧光,且热变性DNA对Tb3+的荧光增敏作用比DNA强几十倍.基于上述实验现象,探讨了四种核苷酸d-AMP,d-CMP,d-GMP,d-TTP及四种核苷dA、dC、dG、dT与Tb3+相互作用的荧光光谱,发现DNA对Tb3+的荧光增敏除与鸟嘌呤碱基有关外,还有可能与磷酸基有关.进一步选择了含有不同数目磷酸基的核苷酸GMP、GDP、GTP及dG研究磷酸基的作用,实验结果表明不含磷酸基的dG不增敏Tb3+的荧光,而分别含有1、2、3个磷酸基的GMP、GDP、GTP三种核苷酸对Tb3+的荧光增敏程度依次为GTP>GDP>GMP,认为DNA对Tb3+的荧光增敏是鸟嘌呤碱基和磷酸基共同作用的结果.

 
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