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p scc
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  p450胆固醇
     The relative expression levels of cholesterol side-chain cleavage enzyme (P450scc) mRNA and 3beta-hydroxysteroid dehydrogenase (3β-HSD) mRNA were determined by RT-PCR.
     RT-PCR法测定P450胆固醇侧链裂解酶(P450scc)mRNA和3β类固醇脱氢酶(3βHSD)mRNA的相对表达量。
     The relative expression levels of cholesterol side-chain cleavage enzyme(P450scc)mRNA and 3beta-hydroxysteroid dehydrogenase(3β-HSD)mRNA were determined by RT-PCR.
     RT-PCR法测定细胞色素P450胆固醇侧链裂解酶(P450scc)mRNA和3β类固醇脱氢酶(3βHSD)mRNA的相对表达量。
短句来源
     However there is no any systematic study about the expression of steroidogenetic enzymes in the goat. In this study, we determined the gene expression of cytochrome P450scc(P450 side chain cleavage), P450arom(cytochrome P450 aromatase) and P450c17(P450 17α-hydroxylase) mRNA during goat ovarian follicular development by RT-PCR and semiquantitative RT-PCR.
     然而,关于山羊类固醇合成酶表达尚未见系统研究。 本实验用RT-PCR方法对P450胆固醇侧链裂解酶(P450scc)、P45017α-羟化酶(P450c17)和P450芳香化酶(P450arom)在山羊卵泡发育和闭锁过程中的表达进行了定位和半定量研究。
短句来源
     P450 Cholesterol side chain cleavage enzyme(P450scc) is a key enzyme for the catalysis of cholesterol into steroid. The activity of P450scc is regulated by Steroidogenic factor 1(SF 1).
     细胞色素P450胆固醇侧链裂解酶(P450scc)是催化胆固醇转化为类固醇的关键酶,其活性受到类固醇生成因子(SF1)的调节。
短句来源
     Objective To observe the change of cytochrome P450 cholesterol side chain catenase (P450scc) expression in testis tissue of adult rats after microwave irradiation.
     目的探讨微波辐射对成年大鼠睾丸组织中细胞色素P450胆固醇侧链裂解酶(P450scc)表达水平的影响。
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  “p 450 scc”译为未确定词的双语例句
     Results The expression of P450scc mRNA in striatum and 3β-HSD mRNA in amygdala, striatum and frontal cortex decreased in group D compared with group C.
     结果与C组相比,D组脑组织P450scc mRNA在纹状体表达降低,3β-HSD mRNA在额叶皮质、纹状体和杏仁核表达降低,W组3β-HSD mRNA在额叶皮质、杏仁核表达降低(P<0.05)。
短句来源
     The effect of Cox7a2 on LH induced testosterone production and the expression of StAR protein,P450scc and 3β-HSD enzymes in TM3 mouse Leydig cells
     Cox7a2对睾酮合成及StAR、P450scc和3β-HSD蛋白表达影响研究
短句来源
     Serum level of T, E2 and the ratio of E2/T were observed, and the mRNA expression levels of StAR, P450scc, IVp-HSDHI and P450arom were determined by RT-PCR.
     观察血清T、E_2水平和血清E_2/T比值,并用RT-PCR测定大鼠睾丸StAR、P450scc、17β-HSDⅢ以及P450arom mRNA水平。
短句来源
     Results: Valprovate caused increase of testosterone (P<0.05), and significantly increased of CYP17 (P<0.01),P450scc (P<0.01) and StAR (P<0.01) mRNA expression were observed in cultured cells.
     结果:VPA刺激了卵泡内膜细胞睾酮的分泌(P<0.05),且CYP17(P<0.01)、P450scc(P<0.01)和StAR(P<0.01)mRNA表达增加。
短句来源
     The relative expression of P450scc and 3β-HSD mRNA did not change markedly. The relative expression of Cyp17 in 0.1mg/(kg·d) group rose markedly;
     P450scc和3β-HSD mRNA相对表达无显著性变化,Cyp17在0.1mg/(kg·d)组的相对表达有显著性升高;
短句来源
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  相似匹配句对
     P450 and Cyt.
     P450、Cyt.
短句来源
     Cytokines and Cytochrome P450
     细胞因子与细胞色素P450
短句来源
     The detecting wavelength was 450nm.
     检测波长450nm。
短句来源
     450±1.2?
     女性为 97.64 5 0± 1.2 42 6。
短句来源
     Electrochemical Researches in SCC
     应力腐蚀的电化学研究
短句来源
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  p scc
Recently, two cytochromes P450 each catalyzing a specified reaction have been purified to a homogeneous state, that is, P450scc having cholesterol side chain cleavage activity and P45011β having 11β-hydroxylation activity.
      
The CYP11A1 gene encodes the cholesterol side-chain cleavage enzyme, also termed cytochrome P450scc, which catalyzes the conversion of cholesterol to pregnenolone in the first step of steroid biosynthesis in mitochondria.
      
In vitellogenic eel ovary, P450scc and P450c17 immunoreactive cells were localized as clusters in the outer layer of the ovarian follicle.
      
In eel testis, P450scc and P450c17 were immunolocalized as clusters in Leydig cells.
      
Antibodies against P450scc, P450c17 and P450arom were generated using recombinant proteins.
      
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To investigate the effects of berberine (Ber) on intestinal hypersecretion induced by cholera toxin (ChT). Methods: Ussing Chamber technique was used to measure the potential difference (PD), short-circuit current (SCC), and the resistance (R) was calculated in isolated guinea-pig ileum. The secretory diarrhoea model was made with ChT to investigate the effects of ChT and ChT + Ber on PD、SCC and R. Results: The PD and SCC in isolated ileum of guinea-pig treated by ChT were significantly greater...

To investigate the effects of berberine (Ber) on intestinal hypersecretion induced by cholera toxin (ChT). Methods: Ussing Chamber technique was used to measure the potential difference (PD), short-circuit current (SCC), and the resistance (R) was calculated in isolated guinea-pig ileum. The secretory diarrhoea model was made with ChT to investigate the effects of ChT and ChT + Ber on PD、SCC and R. Results: The PD and SCC in isolated ileum of guinea-pig treated by ChT were significantly greater than those in ileum of normal guines-pig (P PD 、P SCC <0.01). The PD and SCC were decreased by 1.0mmol/L Ber in mucosal face, 0.1mmol/L and 0.5mmol/L Ber in serosal face (P PD 、P SCC <0.01), but the R was not altered (P>0.05). However, 1.0mmol/L Ber in serosal face not only decreased the PD and SCC (P PD 、P SCC <0.01), but also decreased the R (<0.05). Conclusion: Ber can reversed intestinal hypersecretion induced by ChT.

目的 :探讨小檗碱 (Ber)对分胁性腹泻豚鼠离体回肠电解质转远的影响。方法 :利用霍乱毒素 (ChT)制成分必性腹泻模型 ,通过UssingChamber技术测定豚鼠离体回肠膜电位 (FD)、膜短路电流 (SCC)和膜电阻 (R)。 结果 :(1)分泌性腹泻时 ,豚鼠离体回肠的FD和SCC均显著高于正常对照组 ;(2 )加于粘膜倒 (M )溶液中 1 0mmol/L的Ber和加于浆膜侧 (S)溶液中 0 .1mmol/L及 0 5mmol/L的Ber均可降低PD和SCC ,但不改变R ;而加于S侧溶液中 1.0mmol/L的Ber不仅可降低PD和SCC ,还可降低R。结论 :Ber可抑制由ChT所效的电解质分泌亢进。

Objective To study the protein components of the secretion of the proximal trunk of transected peripheral nerve and the effect of the secretion on the growth of motor neuron.Methods After the sciatic nerves of rats were transected, the proximal trunk secretion collecting compartment (P-SCC), distal trunk SCC (D-SCC) and (proximal) plus distal trunk SCC (PD-SCC) were set up. The protein components of the solution in the 3 types SCC were analyzed by two-dimensional polyacrylamide...

Objective To study the protein components of the secretion of the proximal trunk of transected peripheral nerve and the effect of the secretion on the growth of motor neuron.Methods After the sciatic nerves of rats were transected, the proximal trunk secretion collecting compartment (P-SCC), distal trunk SCC (D-SCC) and (proximal) plus distal trunk SCC (PD-SCC) were set up. The protein components of the solution in the 3 types SCC were analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrum. And the effect of 3 kinds solution on the growth of motor neuron was tested by the motor neuron culture respectively. (Results) All the P-SCC, D-SCC and PD-SCC were filled with light yellow, clear fluids. The protein (concentration) was (5.45±1.2)mg/ml,(3.87±0.7)mg/ml and (5.68±1.5)mg/ml in the solution of PD-SCC, P-SCC and D-SCC (respectively). The protein patterns of the 3 solution were similar, and the detected (protein) spots were 1098±34,985±47 and 1021±36 spots in the solution of P+D-SCC, P-SCC and D-SCC (respectively). Most of the protein spots (distributed) in the rangs of MV 25-90KD and PH 4-8. The results of motor neuron culture demonstrate that the number of survival cells, cell area, neurite length and OD value of experimental groups(including mix group, (proximal) group and distal group) are generally higher than control group. But there is no obvious difference between the three experimental groups. Conclusions (1)The proximal trunk of transected sciatic nerve had the similar secretive ability as the distal nerve trunk.(2)(Protein) image of the 3 kinds of SCC solution showed that proteins from the three SCC solution were homologous. (3)All the 3 kinds of SCC solution could promote the growth and activity of motor neuron, and there was no difference in function between P-SCC and D-SCC solution.

目的 探讨横断性周围神经近侧神经的分泌功能 ,分泌液的蛋白质成分及其对运动神经元生长的影响。方法 建立大鼠坐骨神经横断后神经近端室、神经远端室以及混合室 (近加远端神经 )收集室模型。用蛋白质双向电泳技术和质谱分析法分析 3种收集室内液体 (收集液 )的蛋白质成分 ,并以 3种收集液培养脊髓前角运动神经元。结果 大鼠坐骨神经断端的混合室、近端室和远端室均有淡黄色清亮液体 ,其蛋白质浓度分别为 ( 5 .45± 1.2 )mg/ml ,( 3 .87± 0 .7)mg/ml和 ( 5 .68± 1.5 )mg/ml。收集液蛋白质双向电泳结果显示 3组蛋白质图谱的图案模式相似。混合室、近端室和远端室组分别检测到 10 98± 3 4,985± 47和 10 2 1± 3 6个点。蛋白质点主要分布在pH4~ 8,分子量在2 5~ 90kD的范围内。应用 3组神经断端收集液培养运动神经元发现 ,3种收集液所培养的运动神经元的细胞存活数、神经元的胞体面积、突起长度总体上大于对照组 ;实验组之间无明显差异。结论 ( 1)大鼠坐骨神经横断后 ,其近端神经干具有与远端神经干相似的分泌功能 ;( 2 )蛋白质双向电泳图谱的图案...

目的 探讨横断性周围神经近侧神经的分泌功能 ,分泌液的蛋白质成分及其对运动神经元生长的影响。方法 建立大鼠坐骨神经横断后神经近端室、神经远端室以及混合室 (近加远端神经 )收集室模型。用蛋白质双向电泳技术和质谱分析法分析 3种收集室内液体 (收集液 )的蛋白质成分 ,并以 3种收集液培养脊髓前角运动神经元。结果 大鼠坐骨神经断端的混合室、近端室和远端室均有淡黄色清亮液体 ,其蛋白质浓度分别为 ( 5 .45± 1.2 )mg/ml ,( 3 .87± 0 .7)mg/ml和 ( 5 .68± 1.5 )mg/ml。收集液蛋白质双向电泳结果显示 3组蛋白质图谱的图案模式相似。混合室、近端室和远端室组分别检测到 10 98± 3 4,985± 47和 10 2 1± 3 6个点。蛋白质点主要分布在pH4~ 8,分子量在2 5~ 90kD的范围内。应用 3组神经断端收集液培养运动神经元发现 ,3种收集液所培养的运动神经元的细胞存活数、神经元的胞体面积、突起长度总体上大于对照组 ;实验组之间无明显差异。结论 ( 1)大鼠坐骨神经横断后 ,其近端神经干具有与远端神经干相似的分泌功能 ;( 2 )蛋白质双向电泳图谱的图案显示 3种收集室的蛋白质具有同源性 ;( 3 ) 3种收集液都能促进运动神经元的存活和生长 ,且近端室和远端室收集液的作用没有差别。

 
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