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-pyridoxal phosphate
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  “5 '-pyridoxal phosphate”译为未确定词的双语例句
     The immobilization of enzymes containing 5'-pyridoxal phosphate as coenzyme
     含5′-磷酸吡哆醛(辅因子)的酶的固定化
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  相似匹配句对
     The immobilization of enzymes containing 5'-pyridoxal phosphate as coenzyme
     含5′-磷酸吡哆醛(辅因子)的酶的固定化
短句来源
     (5) .
     (5).
短句来源
     (5).
     6ml及玻璃体腔内填充0.5~3m!
短句来源
     Study on Coenzyme Function of Macromolecular Derivatives of 5-pyridoxal Phosphate
     5′-磷酸吡哆醛高分子衍生物的辅酶功效
短句来源
     Determination of Cytidine-5'-phosphate by Spectrophotometry
     分光光度法测定胞苷-5′-磷酸
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Adult worms of Schistosoma japonicum, collected from experimentally infected rabbits, were homogenized and the homogenate was incubated with 0.017M L-ornithine and 0.017M α-ketoglutarate at 37℃, the pH of the medium was 8.0. The ornithine transaminase activity was expressed in micromoles of glutamic acid produced per mg. nitrogen per hour. The average values of enzymic activity were found to be 33.9 for male worms and 29.0 for female worms. The pH optimum for the enzyme was 8.0-8.2. Using the homogenate of paired...

Adult worms of Schistosoma japonicum, collected from experimentally infected rabbits, were homogenized and the homogenate was incubated with 0.017M L-ornithine and 0.017M α-ketoglutarate at 37℃, the pH of the medium was 8.0. The ornithine transaminase activity was expressed in micromoles of glutamic acid produced per mg. nitrogen per hour. The average values of enzymic activity were found to be 33.9 for male worms and 29.0 for female worms. The pH optimum for the enzyme was 8.0-8.2. Using the homogenate of paired worms, the activities of ornithine transaminase, glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetio transaminase (GOT) were determined respectively. The activity of the first enzyme was 1.5 times greater than that of GPT and 2.4 times greater than that of GOT. When α-ketoglutarate, pyruvate, oxaloacetate or glyoxalate was used as one of the substrates and L-ornithine as the other, the ratio of enzymic activities observed was 100:6.2:1.8:4.2. The worm ornithine transaminase was activated by pyridoxal phosphate and depressed by hydroxylamine. Cupric chloride and p-chloromercu-ribenzoate had a notable inhibitory effect, at 2×10~(-5)M they caused 78.5% and 31.6% inhibition respectively. Norvaline and valine exhibited a competitive inhibition, its action increased significantly as the concentration of L-ornithine was decreased. Inhibition by cucurbitine was noncompetitive, 0.017M of this amino acid inhibited 30% of the enzymic activity. 10~(-3)M tartar emetic and 10~(-4) M furapromidium (F-30066) did not show any effect. Arginine transaminase could not be detected in Schistosoma japonicum by the present method. When the homogenate was incubated with arginine and α-ketoglutarate, the glutamic acid produoed is most likely the result of the combined actions of arginase and ornithine transaminase.

从人工感染的家兎中取得日本血吸虫成虫,制成匀浆后在37℃测定鸟氨酸转氨酶活力,底物L-鸟氨酸及α-酮戊二酸的浓度各为0.017M,pH8.O。测定结果用微克分子/ 毫克氮/小时表示,测得酶活力的平均值雄虫为33.9,雌虫为29.0。此酶的最适pH为8.O—8.2。在对此测定中合抱虫的鸟氨酸转氨酶活力为谷氨酸丙酮酸转氨酸活力的1.5倍,谷氨酸草酰乙酸转氨酶活力的2.4倍。当用鸟氨酸分别与α-酮戊二酸、丙酮酸、草酰乙酸及乙醛酸进行测定时,测得的酶活力比值依次为100:6.2:1.8:4.2。磷酸吡哆醛能增高酶活力,羟胺则使之降低。氯化铜及对氯汞苯甲酸具有很强的抑制作用,在2×10~(-5)M的浓度下前者抑制78.5%,后者抑制31.6%。正缬氨酸及缬氨酸对此酶的抑制作用是竞争性的,当底物鸟氨酸的浓度减低时,抑制作用增强。南瓜子氨酸的抑制是非竞争性的,在O.017M的浓度下抑制酶活力29.5%。酒石酸锑钾(10~(-3)M)及呋喃丙胺(10~(-4)M)对酶活力无影响。在日本血吸虫中测不出精氨酸转氨酶的存在,以精氨酸为底物作转氨酶测定时所产生的谷氨酸是由于精氨酸酶和鸟氨酸转氨酶相继作用的结果。

Glutamate decarboxylase from E. Coli catalyzes the decarboxytation of L-glutamate to yield γ-aminobutyric acid, the production of which will be more efficient if the enzyme reaction is conducted in immobilized cell system. It is possible now to immobilize the cell glutamate decarboxylase through entrapping the E. Coli As 1.505 whole cell into the alginate glutaric dialdehyde gel. However, the enzyme is subjected to degeneration due to the loss of a cofactor, pyridoxal phosphate (PLP) in the course of the...

Glutamate decarboxylase from E. Coli catalyzes the decarboxytation of L-glutamate to yield γ-aminobutyric acid, the production of which will be more efficient if the enzyme reaction is conducted in immobilized cell system. It is possible now to immobilize the cell glutamate decarboxylase through entrapping the E. Coli As 1.505 whole cell into the alginate glutaric dialdehyde gel. However, the enzyme is subjected to degeneration due to the loss of a cofactor, pyridoxal phosphate (PLP) in the course of the enzyme reaction. By treating the entrapped cell in a 0.05 mM PLP solution at 37℃ for 2 hours we succeded in regenerating the enzyme activities to 100% of its original.

用海藻酸钠—戊二醛法固定产谷氨酸脱羧酶的大肠杆菌As 1.505细胞,在37℃0.2 MpH 4.0醋酸缓冲液中,能催化L-谷氨酸(glu)裂解为γ-氨基丁酸和二氧化碳(CO_2)。固定化细胞在催化反应过程中由于部分辅酶—磷酸吡哆醛(PLP)的脱落而导致酶活力的下降,因此影响了反复使用。本法将固定化细胞在37℃0.05mM PLP溶液中保温2小时,酶活力便获得100%的恢复。

Purified PEP carboxylase from sorghum leaves was inactivated by amino group reagents, 2, 4, 6-trinitrobenzene sulfonate (TNBS) and pyridoxal phosphate (PLP). The inactivation kinetics of the enzyme by TNBS was first order with respect to incubation time and inhibitor concentration. Kinetic date indicated that only one molecule of TNBS was involved in binding to the enzyme during the inactivation process.Observation of the absorption spectrum of the modified enzyme showed that the modified amino acid residues...

Purified PEP carboxylase from sorghum leaves was inactivated by amino group reagents, 2, 4, 6-trinitrobenzene sulfonate (TNBS) and pyridoxal phosphate (PLP). The inactivation kinetics of the enzyme by TNBS was first order with respect to incubation time and inhibitor concentration. Kinetic date indicated that only one molecule of TNBS was involved in binding to the enzyme during the inactivation process.Observation of the absorption spectrum of the modified enzyme showed that the modified amino acid residues of the enzyme were lysyl. Substrate (PEP) and activator (G6P) protected the enzyme against TNBS-inactivation. Spectrophotometric kinetics revealed that in the presence of G6P, lysyl residues of the enzyme were modified more slowly than in its absence. The dissociation constant of G6P-enzyme complex was determined according to Scrutton and Utter's equation, K_d=2.39×10~(-3) M. Bicarbonate, magnesium ion had no influence upon the TNBS-inactivation of enzyme separately, but together, they accelerated the inactivation.During modification of the enzyme by TNBS, the inactivation was accompanied by desensitization to G6P. In contrast, the sensitivity to glycine was substantially retained.

本文报道纯化的高粱叶片PEP羧化酶经氨基修饰剂TNBS和PLP的修饰迅速失活。酶的TNBS失活与保温时间和抑制剂浓度呈函数关系并表现为拟一级反应的特性。动力学资料表明酶仅被1分子TNBS修饰即失活。TNBS修饰酶的吸收光谱特性表明被修饰的是酶蛋白的赖氨酸残基。底物(PEP)和效应剂(G6P)保护酶免被TNBS失活。计算G6P和酶的解离常数K_d=2.39×10~(-3)M。酶的其他反应组分HCO_3~-和MgCl_2单独存在时均不影响TNBS对酶的失活作用。在被TNBS修饰过程中还导致酶对G6P迅速脱敏,同时却保持酶对甘氨酸的敏感性。

 
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